Heat stress activates the <scp>A</scp>kt/m<scp>TOR</scp> signalling pathway in rat skeletal muscle

Yoshihara, Toshinori; Naito, Hisashi; Kakigi, Ryo; Ichinoseki‐Sekine, Noriko; Ogura, Yuji; Sugiura, T.; Katamoto, Shizuo

doi:10.1111/apha.12040

Cited by 95 publications

(88 citation statements)

References 53 publications

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“…In contrast to our findings, a previous study reported that HS for 30 min activated p70S6K in rat skeletal muscle in vivo 10) . These contrasting results may be attributed to differences in the heat exposure process.…”

Section: Fig 2 Changes In Buffer Ph During Incubationcontrasting

confidence: 99%

“…4E-BP1 phosphorylation and protein synthesis are suppressed during resistance-or endurancetype exercise 1,2) , which may be attributed to the negative regulation of mTOR by AMPK 9) . In contrast, it has been shown that HS activates the mTOR pathway in skeletal muscle 10) and thereby contributes the prevention of skeletal muscle atrophy 11) . However, the direct effect of shortterm HS on protein synthesis in skeletal muscle remains unclear.…”

Section: Introductionmentioning

confidence: 94%

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The effect of short-term heat stress on protein synthesis signaling in isolated rat skeletal muscle

Goto

Sekine

Oshima

et al. 2018

JPFSM

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Heat stress (HS) is a potent stimulus for activating glucose metabolism in skeletal muscles. However, the effect of short-term HS on protein turnover in skeletal muscles is unclear. This study aimed to investigate the effect of short-term HS on protein synthesis and protein degradation in skeletal muscles. The epitrochlearis muscle was isolated from male Sprague-Dawley rats weighing 150-160 grams (g) and incubated with or without HS at 42°C for 10 or 30 min in alpha minimum essential medium. HS for 30 min significantly decreased phosphorylation of 70-kDa ribosomal protein S6 kinase at Thr 389 and 4E-binding protein 1 at Thr 37/46 . Correspondingly, HS for 30 min decreased the rate of protein synthesis. In contrast, HS had no effect on the expression of autophagy-related proteins, including microtubule-associated protein light chain 3 and p62, or on the mRNA expression of muscle-specific ubiquitin ligases, including muscle RING-finger 1 (MuRF1) and atrogin-1/MAFbx. These findings suggested that short-term HS for approximately 30 min is a physiologically relevant stimulus that suppresses protein synthesis signaling in skeletal muscles.

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Section: Fig 2 Changes In Buffer Ph During Incubationcontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 94%

The effect of short-term heat stress on protein synthesis signaling in isolated rat skeletal muscle

Goto

Sekine

Oshima

et al. 2018

JPFSM

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“…5). We and others have reported increased circulating insulin concentrations [15], [16] and signaling [17] in response to acute postnatal HS in a variety of models. While the reason for enhanced circulating insulin during HS response remains unclear, it likely includes insulin's role in activating the cellular stress response [48].…”

Section: Discussionmentioning

confidence: 72%

“…Although the mechanisms responsible for HS induced alterations in body composition are not completely understood, it may be partially explained by our recent finding that basal insulin increased in a variety of HS models [15] including pigs [16]. Additionally, it was recently reported in a rodent model that HS stimulates insulin signaling in skeletal muscle [17]. The increase in insulin (a potent anabolic signal) occurs despite the marked reduction in feed intake and hyper-catabolic hormonal milieu that dominates during HS [1], [15].…”

Section: Introductionmentioning

confidence: 99%

Gestational Heat Stress Alters Postnatal Offspring Body Composition Indices and Metabolic Parameters in Pigs

et al. 2014

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The study objectives were to test the hypothesis that heat stress (HS) during gestational development alters postnatal growth, body composition, and biological response to HS conditions in pigs. To investigate this, 14 first parity crossbred gilts were exposed to one of four environmental treatments (TNTN, TNHS, HSTN, or HSHS) during gestation. TNTN and HSHS dams were exposed to thermal neutral (TN, cyclical 18–22°C) or HS conditions (cyclical 28–34°C) during the entire gestation, respectively. Dams assigned to HSTN and TNHS treatments were heat-stressed for the first or second half of gestation, respectively. Postnatal offspring were exposed to one of two thermal environments for an acute (24 h) or chronic (five weeks) duration in either constant TN (21°C) or HS (35°C) environment. Exposure to chronic HS during their growth phase resulted in decreased longissimus dorsi cross-sectional area (LDA) in offspring from HSHS and HSTN treated dams whereas LDA was larger in offspring from dams in TNTN and TNHS conditions. Irrespective of HS during prepubertal postnatal growth, pigs from dams that experienced HS during the first half of gestation (HSHS and HSTN) had increased (13.9%) subcutaneous fat thickness compared to pigs from dams exposed to TN conditions during the first half of gestation. This metabolic repartitioning towards increased fat deposition in pigs from dams heat-stressed during the first half of gestation was accompanied by elevated blood insulin concentrations (33%; P = 0.01). Together, these results demonstrate HS during the first half of gestation altered metabolic and body composition parameters during future development and in biological responses to a subsequent HS challenge.

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“…Moreover, heat stress affects protein anabolism and catabolism as well as satellite cell proliferation, all of which play important roles in determining muscle growth and development4. In addition, heat stress activates signaling pathways associated with the regulation of skeletal muscle growth and development56.…”

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confidence: 99%

Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing

Hao

Cui

2016

Sci Rep

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Heat stress affects muscle development and meat quality in food animals; however, little is known regarding its regulatory mechanisms at the epigenetic level, such as via DNA methylation. In this study, we aimed to compare the DNA methylation profiles between control and heat-stressed pigs to identify candidate genes for skeletal muscle development and meat quality. Whole-genome bisulfite sequencing was used to investigate the genome-wide DNA methylation patterns in the longissimus dorsi muscles of the pigs. Both groups showed similar proportions of methylation at CpG sites but exhibited different proportions at non-CpG sites. A total of 57,147 differentially methylated regions were identified between the two groups, which corresponded to 1,422 differentially methylated genes. Gene ontogeny and KEGG pathway analyses indicated that these were mainly involved in energy and lipid metabolism, cellular defense and stress responses, and calcium signaling pathways. This study revealed the global DNA methylation pattern of pig muscle between normal and heat stress conditions. The result of this study might contribute to a better understanding of epigenetic regulation in pig muscle development and meat quality.

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Heat stress activates the Akt/mTOR signalling pathway in rat skeletal muscle

Abstract: Our data indicate that the altered temperature increased phosphorylation in a temperature-dependent manner in rat skeletal muscle and may itself be a key stimulator of Akt/mTOR signalling.

Cited by 95 publications

References 53 publications

The effect of short-term heat stress on protein synthesis signaling in isolated rat skeletal muscle

The effect of short-term heat stress on protein synthesis signaling in isolated rat skeletal muscle

Gestational Heat Stress Alters Postnatal Offspring Body Composition Indices and Metabolic Parameters in Pigs

Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing

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