Heat‐Stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration

Oishi, Yasuharu; Roy, Roland R.; Ogata, Toru; Ohira, Yoshinobu

doi:10.1002/mus.24686

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…Our results show that exposure to HT immediately after and for 4 consecutive days after a maximal bout of eccentric exercise in humans hastens recovery of fatigue resistance and tends to reduce perceived soreness. These findings are in line with a growing body of literature supporting a beneficial impact of HT on recovery after noninjurious exercise (8) as well as severe muscle injuries (19,32,33,44,48). Additional studies are warranted to determine whether repeated exposure to local HT amplifies the skeletal muscle adaptations to exercise training in humans.…”

Section: Discussionsupporting

confidence: 84%

Impact of heat therapy on recovery after eccentric exercise in humans

Kim

Kuang

Song

et al. 2019

Journal of Applied Physiology

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The purpose of this study was to investigate the effects of heat therapy (HT) on functional recovery, the skeletal muscle expression of angiogenic factors, macrophage content, and capillarization after eccentric exercise in humans. Eleven untrained individuals (23.8 ± 0.6 yr) performed 300 bilateral maximal eccentric contractions of the knee extensors. One randomly selected thigh was treated with five daily 90-min sessions of HT, whereas the opposite thigh received a thermoneutral intervention. Peak isokinetic torque of the knee extensors was assessed at baseline and daily for 4 days and fatigue resistance was assessed at baseline and 1 and 4 days after the eccentric exercise session. Muscle biopsies were obtained 2 wk before and 1 and 5 days after the eccentric exercise bout. There were no differences between thighs in the overall recovery profile of peak torque. However, the thigh exposed to HT had greater fatigue resistance than the thigh exposed to the thermoneutral intervention. The change from baseline in mRNA expression of vascular endothelial growth factor (VEGF) was higher at day 1 in the thigh exposed to HT. Protein levels of VEGF and angiopoietin 1 were also significantly higher in the thigh treated with HT. The number of capillaries around type II fibers decreased similarly in both thighs at day 5. Exposure to HT had no impact on macrophage content. These results suggest that HT accelerates the recovery of fatigue resistance after eccentric exercise and promotes the expression of angiogenic factors in human skeletal muscle. NEW & NOTEWORTHY We investigated whether exposure to local heat therapy (HT) accelerates recovery after a bout of eccentric exercise in humans. Compared with a thermoneutral control intervention, HT improved fatigue resistance of the knee extensors and enhanced the expression of the angiogenic mediators vascular endothelial growth factor and angiopoietin 1. These results suggest that HT hastens functional recovery and enhances the expression of regulatory factors involved in muscle repair after eccentric exercise in humans.

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Section: Discussionsupporting

confidence: 84%

Impact of heat therapy on recovery after eccentric exercise in humans

Kim

Kuang

Song

et al. 2019

Journal of Applied Physiology

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“…Recently, hyperthermal therapy has attracted increasing attention in the fields of tissue engineering and cancer chemo-therapeutics due to its potential to modify the extracellular microenvironment, and thus regulate localized tissue responses including immunological reaction, tissue perfusion, and tissue oxygenation [4, 5]. Although controlled thermal delivery of heat has shown some beneficial effects on myogenesis during skeletal muscle repair in both in vitro [6–8] and in vivo studies [9–11], the detailed and coordinated effects of thermal treatment on muscle regeneration remain under characterized, limiting the development of a tailored hyperthermia treatment protocol for muscle regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…Myofibrillogenesis, the development of the myofibril during myogenesis, plays a critical role in controlling the contractile strength of skeletal muscles [14, 15]. Recently, Yamaguchi et al [6] and Oishi et al [9] reported a fast-to-slow fiber-type shift in myotubes or myofibers during myogenesis in their in vitro and in vivo studies, respectively. Yet, their work solely focused on analyzing the expressions of myosin heavy chains.…”

Section: Introductionmentioning

confidence: 99%

Controlled Heat Stress Promotes Myofibrillogenesis during Myogenesis

Guo¹,

Miller²,

An³

et al. 2016

PLoS ONE

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Hyperthermia therapy has recently emerged as a clinical modality used to finely tune heat stress inside the human body for various biomedical applications. Nevertheless, little is known regarding the optimal timing or temperature of heat stress that is needed to achieve favorable results following hyperthermia therapy for muscle regeneration purposes. The regeneration of skeletal muscle after injury is a highly complex and coordinated process that involves a multitude of cellular mechanisms. The main objective of this study was to characterize the effects of hyperthermal therapy on the overall behavior of myoblasts during myogenic differentiation. Various cellular processes, including myogenesis, myofibrillogenesis, hypertrophy/atrophy, and mitochondrial biogenesis, were studied using systematic cellular, morphological, and pathway-focused high-throughput gene expression profiling analyses. We found that C2C12 myoblasts exhibited distinctive time and temperature-dependence in biosynthesis and regulatory events during myogenic differentiation. Specifically, we for the first time observed that moderate hyperthermia at 39°C favored the growth of sarcomere in myofibrils at the late stage of myogenesis, showing universal up-regulation of characteristic myofibril proteins. Characteristic myofibrillogenesis genes, including heavy polypeptide 1 myosin, heavy polypeptide 2 myosin, alpha 1 actin, nebulin and titin, were all significantly upregulated (p<0.01) after C2C12 cells differentiated at 39°C over 5 days compared with the control cells cultured at 37°C. Furthermore, moderate hyperthermia enhanced myogenic differentiation, with nucleus densities per myotube showing 2.2-fold, 1.9-fold and 1.6-fold increases when C2C12 cells underwent myogenic differentiation at 39°C over 24 hours, 48 hours and 72 hours, respectively, as compared to the myotubes that were not exposed to heat stress. Yet, atrophy genes were sensitive even to moderate hyperthermia, indicating that strictly controlled heat stress is required to minimize the development of atrophy in myotubes. In addition, mitochondrial biogenesis was enhanced following thermal induction of myoblasts, suggesting a subsequent shift toward anabolic demand requirements for energy production. This study offers a new perspective to understand and utilize the time and temperature-sensitive effects of hyperthermal therapy on muscle regeneration.

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“…Yamaguchi et al (40) also reported that continuous heat stress (39°C for 6 h) caused differentiation and a fast-to-slow fiber-type transition in mammalian myoblasts. Similarly, Oishi et al (25) reported that intermittent application of heat stress (42°C for 30 min on alternating days) to injured rat soleus muscle promoted a fast-to-slow transformation of MyHC phenotypes at an early phase of regeneration after injury. In agreement with these findings, the present study revealed that the timing of the disappearance of MyHC neo and IIb isoforms in regenerating soleus muscles was accelerated by heat stress at the whole-muscle level (Fig.…”

Section: Discussionmentioning

confidence: 89%

Impact of different temperature stimuli on the expression of myosin heavy chain isoforms during recovery from bupivacaine-induced muscle injury in rats

Shibaguchi

Hoshi

Yoshihara

et al. 2019

Journal of Applied Physiology

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Limited information exists regarding the impact of different temperature stimuli on myosin heavy chain (MyHC) expression in skeletal muscle during recovery from injury. Therefore, this experiment investigated the impact of both cold and heat exposure on the MyHC isoform profile in the rat soleus during recovery from injury. Male Wistar rats were randomly divided into control, bupivacaine-injected (BPVC), BPVC with icing, and BPVC with heat stress groups. Muscle injury was induced by intramuscular injection of bupivacaine into soleus muscles of male Wistar rats. Icing treatment (0°C for 20 min) was performed immediately after the injury. Intermittent heat stress (42°C for 30 min on alternating days) was carried out during 2–14 days after bupivacaine injection. In response to injury, a transient increase in developmental, IId/x, and IIb MyHC isoforms, as well as various types of hybrid fibers, followed by the recovery of the MyHC profile toward the control level, was noted in the regeneration of the soleus. The restoration of the MyHC profile in the regenerating muscle at whole-muscle and individual myofiber levels was partially delayed by icing but facilitated by heat stress. In addition, the application of repeated heat stress promoted the recovery of soleus muscle mass toward the control level following injury. We conclude that compared with acute and immediate cold (icing) treatment, chronic and repeated heat stress may be a more appropriate treatment for the enhancement of both normalization of the MyHC profile and restoration of muscle mass following injury. NEW & NOTEWORTHY Cold exposure (icing), but not heat exposure, has been well accepted as a first-aid treatment for accidental and/or sports-related injuries. However, recent evidence suggests the negative impact of icing treatment on skeletal muscle regeneration following injury. Here, we demonstrated that acute/immediate icing treatment delayed the restoration of the myosin heavy chain (MyHC) profile, but intermittent hyperthermia, repeated for several days, facilitated the recovery of both muscle mass and the MyHC profile in the regeneration of skeletal muscle following injury.

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Heat‐Stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration

Abstract: The results indicate that heat stress accelerates fast-to-slow MyHC phenotype conversion in regenerating fibers via activation of satellite cells.

Cited by 10 publications

References 29 publications

Impact of heat therapy on recovery after eccentric exercise in humans

Impact of heat therapy on recovery after eccentric exercise in humans

Controlled Heat Stress Promotes Myofibrillogenesis during Myogenesis

Impact of different temperature stimuli on the expression of myosin heavy chain isoforms during recovery from bupivacaine-induced muscle injury in rats

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