Heat Stress Reduces the Susceptibility of Caenorhabditis elegans to Orsay Virus Infection

Huang, Yuqing; Sterken, M.G.; Zwet, Koen van; Sluijs, Lisa van; Pijlman, Gorben P.; Kammenga, J.E.

doi:10.3390/genes12081161

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…RNA isolation for the time series experiment, RIL experiment was done using the Maxwell® 16 AS2000 instrument with the Maxwell® 16 LEV simply RNA tissue kit . The IL experiment RNA isolation was conducted using the Maxwell® 16 LEV plant RNA kit (Promega) (35). The frozen nematodes were lysed using a modified lysis protocol including a proteinase K digestion (36).…”

Section: Gene Expression Measurementsmentioning

confidence: 99%

eQTL mapping in transgenic alpha-synuclein carryingCaenorhabditis elegansrecombinant inbred line

Huang,

Wang,

van Sluijs

et al. 2023

Preprint

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Protein aggregation of alpha-synuclein (alphaS) is a genetic and neuropathological hallmark of Parkinson disease (PD). Studies in the model nematode Caenorhabditis elegans suggested that variation of alphaS aggregation depends on the genetic background. However, which genes and genetic modifiers underlie individual differences in alphaS pathology remains unknown. To study the genotypic-phenotypic relationship of alphaS aggregation, we constructed a Recombinant Inbred Line (RIL) panel derived from a cross between genetically divergent strains C. elegans NL5901 and SCH4856, both harboring the human alphaS gene. As a first step to discover genetic modifiers 70 alphaS-RILs were measured for whole-genome gene expression and expression quantitative locus analysis (eQTL) were mapped. We detected multiple eQTL hot-spots, many of which were located on Chromosome V. To confirm a causal locus, we developed Introgression Lines (ILs) that contain SCH4856 introgressions on Chromosome V in an NL5901 background. We detected 74 genes with an interactive effect between alphaS and the genetic background, including the human p38 MAPK homologue pmk-1 that has previously been associated with PD. Together, we present a unique alphaS-RIL panel for defining effects of natural genetic variation on alphaS pathology, which contributes to finding genetic modifiers of PD.

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Section: Gene Expression Measurementsmentioning

confidence: 99%

eQTL mapping in transgenic alpha-synuclein carryingCaenorhabditis elegansrecombinant inbred line

Huang,

Wang,

van Sluijs

et al. 2023

Preprint

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“…It has also been shown that hsp genes in C. elegans are induced in response to various stressors, such as oxidative stress [9][10][11], electromagnetic field [12][13][14], immunological stress [15][16][17], or toxic chemical compounds [18][19][20]. However, thermal stress is one of the stress factors most often used in experiments aimed at activating hsp genes in C. elegans [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Temperature Effects on Expression Levels of hsp Genes in Eggs and Second-Stage Juveniles of Meloidogyne hapla Chitwood, 1949

Flis,

Malewski,

Dobosz

2024

IJMS

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Meloidogyne hapla is one of the most important nematode pathogens. It is a sedentary, biotrophic parasite of plants that overwinters in the soil or in diseased roots. The development of M. hapla is temperature dependent. Numerous studies have been performed on the effect of temperature on the development of M. hapla, but only a few of them analyzed the heat shock protein (hsp) genes. The aim of the study was to perform expression profiling of eight hsp genes (Mh-hsp90, Mh-hsp1, Mh-hsp4, Mh-hsp6, Mh-hsp60, Mh-dnj19, Mh-hsp43, and Mh-hsp12.2) at two development stages of M. hapla, i.e., in eggs and second-stage juveniles (J2). The eggs and J2 were incubated under cold stress (5 °C), heat stress (35 °C, 40 °C), and non-stress (10 °C, 20 °C, and 30 °C) conditions. Expression profiling was performed by qPCR. It was demonstrated that only two genes, Mh-hsp60 and Mh-dnj19, have been upregulated by heat and cold stress at both development stages. Heat stress upregulated the expression of more hsp genes than cold stress did. The level of upregulation of most hsp genes was more marked in J2 than in eggs. The obtained results suggest that the Mh-hsp90 and Mh-hsp1 genes can be used as bioindicators of environmental impacts on nematodes of the Meloidogyne genus.

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“…Many of the oldest evolutionary animals suffer from diverse diseases, similar to vertebrate animals. For example, Caenorhabditis elegans and Dugesia japonica can be killed or caused diseased symptoms in the worms by a diverse range of pathogens, such as Pseudomonas aeruginosa , Staphylococcus aureus , and Candida albicans (Bosch et al, 2015 ; Huang et al, 2021 ; Kai et al, 2020 ; Prasad et al, 2014 ). Given their special anatomical structure, animals at different evolutionary levels could be used as model organisms to study the fundamental pathological processes of diseases.…”

Section: Introductionmentioning

confidence: 99%

Dysbiosis of intestinal homeostasis contribute to Whitmania pigra edema disease

Dai

Chen

Qian

et al. 2023

Microbial Biotechnology

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Whitmania pigra is widely used in traditional Chinese medicine. However, W. pigra is being threatened by an edema disease with unknown causes (WPE). In this study, a comprehensive exploration of virome, microbiome, and metabolome aberrations in the intestine of W. pigra was performed to address the aetiology of WPE. Virome analysis indicated that eukaryotic viruses did not contribute to WPE, whereas an expansion of Caudovirales was observed in WPE. Compared to the control, the microbial richness and diversity in diseased W. pigra decreased remarkably. Nine genera, including Aeromonas, Anaerotruncus, Vibrio, Proteocatella, Acinetobacter, and Brachyspira were overrepresented in WPE, whereas eleven genera, including Bifidobacterium, Phascolarctobacterium, Lactobacillus, Bacillus and AF12, were enriched in healthy individuals. Furthermore, certain metabolites, especially amino acids, short‐chain fatty acids, and bile acids, were found to be linked to intestinal microbiota alterations in WPE. An integration of the microbiome and metabolome in WPE found that dysbiosis of the gut microbiota or metabolites caused WPE. Notably, W. pigra accepted intestinal microbiota transplantation from WPE donors developed WPE clinical signs eventually, and the dysbiotic intestinal microbiota can be recharacterized in this recipient W. pigra. Strikingly, pathological features of metanephridium and uraemic toxin enrichment in the gut indicated a putative interconnection between the gut and metanephridium in WPE, which represents the prototype of the gut–kidney axis in mammals. These finding exemplify the conservation of “microecological Koch's postulates” from annelids to insects and other vertebrates, which provides a direction of prevention and treatment for WPE and opens a new insight into the pathogenesis of aquatic animal diseases from an ecological perspective.

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Heat Stress Reduces the Susceptibility of Caenorhabditis elegans to Orsay Virus Infection

Cited by 12 publications

References 38 publications

eQTL mapping in transgenic alpha-synuclein carryingCaenorhabditis elegansrecombinant inbred line

eQTL mapping in transgenic alpha-synuclein carryingCaenorhabditis elegansrecombinant inbred line

Temperature Effects on Expression Levels of hsp Genes in Eggs and Second-Stage Juveniles of Meloidogyne hapla Chitwood, 1949

Dysbiosis of intestinal homeostasis contribute to Whitmania pigra edema disease

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