Heat tolerance of vancomycin resistant Enterococcus faecium.

Panagea, Stavroula; Chadwick, P R

doi:10.1136/jcp.49.8.687

Cited by 13 publications

(5 citation statements)

References 7 publications

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“…Among the HAIs caused by enterococcal species are catheter-associated urinary tract infections (CAUTI), as well as noncatheter-associated urinary tract infections (UTI), infective endocarditis (IE), and septicemia ( 10 , 11 ). The prevention and treatment of enterococcal infections in the clinical setting has been increasingly difficult due to: i) the ability of enterococci to withstand heat ( 12 , 13 ), UV radiation ( 14 ), and aseptic solutions, such as chloride and alcohol preparations ( 15 , 16 ); ii) their inherent resistance to antimicrobial agents, such as cephalosporins, monobactams, penicillins and aminoglycosides ( 17 , 18 ); iii) their ability to form antibiotic resistant biofilms ( 19 – 21 ); and iv) their capacity to acquire antibiotic resistance genes, such as those that encode proteins giving resistance to vancomycin ( 22 – 24 ). Thus, new and innovative therapeutics to combat this serious threat are needed.…”

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confidence: 99%

Ring-fused 2-pyridones effective against multidrug-resistant Gram-positive pathogens and synergistic with standard-of-care antibiotics

Nye

Tükenmez

Singh

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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The alarming rise of multidrug-resistant Gram-positive bacteria has precipitated a healthcare crisis, necessitating the development of new antimicrobial therapies. Here we describe a new class of antibiotics based on a ring-fused 2-pyridone backbone, which are active against vancomycin-resistant enterococci (VRE), a serious threat as classified by the Centers for Disease Control and Prevention, and other multidrug-resistant Gram-positive bacteria. Ring-fused 2-pyridone antibiotics have bacteriostatic activity against actively dividing exponential phase enterococcal cells and bactericidal activity against nondividing stationary phase enterococcal cells. The molecular mechanism of drug-induced killing of stationary phase cells mimics aspects of fratricide observed in enterococcal biofilms, where both are mediated by the Atn autolysin and the GelE protease. In addition, combinations of sublethal concentrations of ring-fused 2-pyridones and standard-of-care antibiotics, such as vancomycin, were found to synergize to kill clinical strains of VRE. Furthermore, a broad range of antibiotic resistant Gram-positive pathogens, including those responsible for the increasing incidence of antibiotic resistant healthcare-associated infections, are susceptible to this new class of 2-pyridone antibiotics. Given the broad antibacterial activities of ring-fused 2-pyridone compounds against Gram-positive (GmP) bacteria we term these compounds GmPcides, which hold promise in combating the rising tide of antibiotic resistant Gram-positive pathogens.

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confidence: 99%

Ring-fused 2-pyridones effective against multidrug-resistant Gram-positive pathogens and synergistic with standard-of-care antibiotics

Nye

Tükenmez

Singh

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…The presence of E. faecium is undesirable mainly because the bacterium easily develops resistance to antibiotics (vancomycin, tetracycline), see e.g. Panagea and Chadwick (1996). Novais et al (2005) and Nishimoto et al (2005) described resistant strains isolated in hospital environments.…”

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Thermal inactivation of Enterococcus faecium

Spelina¹,

Schlemmerova²,

Landfeld³

et al. 2007

Czech J. Food Sci.

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AbstractŠpelina V., Schlemmerová L., Landfeld A., Kýhos K., Měřička P., Houška M. (2007): Thermal inactivation of Enterococcus faecium. Czech J. Food Sci., 25: 283-290.Data for thermal inactivation of working suspension of Enterococcus faecium in model solutions were acquired and used to develop a mathematical model for thermal inactivation of the bacterium. The model is valid within the water activity range 0.97 to 0.99; pH range 6.0 to 7.6; temperature range 60°C to 65°C, and was determined for the microorganism concentration ranges of 10 2 per ml to 10 8 per ml of the model inactivation solution. An Excel procedure was developed in Visual Basic language which enables the calculation of the final concentration of the microorganism from the input data for pH, a w , logN 0 , temperature, and holding time of the treatment. The proposed model was verified in experiments using cow and human milks. With cow milk, the correspondence between the experimental and the predicted data is highly satisfactory. With human milk, the model predicts a smaller effect of heating than is that manifested experimentally.

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“…vancomycin, ampicillin, gentamicin, erythromycin, and streptomycin (Panagea et al 1996;Barkholt et al 1997;Citak et al 2005). This causes problems e.g.…”

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High pressure inactivation of Enterococcus faecium - modelling and verification

Landfeld¹,

Strohalm²,

Kýhos³

et al. 2009

Czech J. Food Sci.

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Landfeld A., Strohalm J., Kýhos K., Průchová J., Houška M., Novotná P., Schlemmerová L., Šmuhařová H., Špelina V., Čermák P., Pavlišová K., Měřička P. High-pressure inactivation data were obtained for model working suspensions of Enterococcus faecium in saline solutions in pH range from 5.5 to 6.8, at water activity 0.99 (corresponding to the saline solution). The data were predicted for the pressure range of 450 MPa to 550 MPa, at the initial model suspension temperature 6-7°C prior to pressurising. The results indicate that E. faecium is a highly resistant organism under physiological pH values. With decreasing the substrate pH, the tolerance to the inactivation effects of high pressure decreased. The high-pressure inactivation proved ineffective for the above organism in the range of physiological pH values. The values for high-pressure inactivation model were specified and the calculated parameters of high-pressure inactivation were compared to the experimental data for the working suspensions of E. faecium in cow and human milks. As to cow milk, the predicted data showed some deviation from the model experimental results, while with human milk the model failed completely. High-pressure inactivation of E. faecium in human milk proved to be significantly more effective than that predicted by a model based on the saline solution.

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Heat tolerance of vancomycin resistant Enterococcus faecium.

Cited by 13 publications

References 7 publications

Ring-fused 2-pyridones effective against multidrug-resistant Gram-positive pathogens and synergistic with standard-of-care antibiotics

Ring-fused 2-pyridones effective against multidrug-resistant Gram-positive pathogens and synergistic with standard-of-care antibiotics

Thermal inactivation of Enterococcus faecium

High pressure inactivation of Enterococcus faecium - modelling and verification

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