Heat transfer design of an electrophoresis experiment

Clifton, Michaël; Marsal, O.

doi:10.1016/0094-5765(89)90013-1

Cited by 8 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the observation phase, the temperature of the cooling liquid was adjusted each time the field strength was changed. The heat-transfer properties of the chamber and the liquid circuits had been calculated on the basis of tests performed on the ground and this allowed a suitable cooling temperature to be calculated so that the temperature variations within the chamber were minimized (Clifton and Marsal, 1989).…”

Section: Methodsmentioning

confidence: 99%

Protein separation by continuous‐flow electrophoresis in microgravity

1996

View full text Add to dashboard Cite

During the IML-2 space shuttle mission, the RAMSES instrument was operated in the Spacelab module. This continuous-flow electrophoresis device performs separation and purification of protein solutions on a preparative scale. Samples containing artificial mixtures of pure proteins were used to test the capabilities of the device, and useful separations were obtained for proteins having a mobility difference of only 3 x 10(-9) m2 V-1 s-1. Operating conditions that cannot be applied on earth were explored for two different sample concentrations, one of which is too high to allow treatment on earth. It agrees well with a previously published numerical model in that the main cause of loss in resolution in this process is the electrohydrodynamic spreading of the protein filaments.

show abstract

Section: Methodsmentioning

confidence: 99%

Protein separation by continuous‐flow electrophoresis in microgravity

1996

View full text Add to dashboard Cite

show abstract

“…In 1988, Boese derived a mathematical model of FFE to explore the influence of temperature on the motion of carrier buffers (CBs) and samples . The following year, Clifton and Marsal proposed a mathematical model of heat generation and transfer in an FFE device with circulatory cool water system , and they applied the model to select the cooling temperature to minimize temperature variations in the separation chamber. However, it was found that these investigations could not be directly used for the newly developed FFE device with thermoelectric cooling system .…”

Section: Introductionmentioning

confidence: 99%

Experimental study on the optimization of general conditions for a free‐flow electrophoresis device with a thermoelectric cooler†

Yan

Yang

Zhang

et al. 2014

J of Separation Science

View full text Add to dashboard Cite

With a given free-flow electrophoresis device, reasonable conditions (electric field strength, carrier buffer conductivity, and flow rate) are crucial for an optimized separation. However, there has been no experimental study on how to choose reasonable general conditions for a free-flow electrophoresis device with a thermoelectric cooler in view of Joule heat generation. Herein, comparative experiments were carried out to propose the selection procedure of general conditions in this study. The experimental results demonstrated that appropriate conditions were (i) <67 V/cm electric field strength; (ii) lower than 1.3 mS/cm carrier buffer conductivity (Tris-HCl: 20 mM Tris was titrated by HCl to pH 8.0); and (iii) higher than 3.6 mL/min carrier buffer flow rate. Furthermore, under inappropriate conditions (e.g. 400 V voltage and 40 mM Tris-HCl carrier buffer), the free-flow electrophoresis separation would be destroyed by bubbles caused by more Joule heating. Additionally, a series of applications under the appropriate conditions were performed with samples of model dyes, proteins (bovine serum albumin, myoglobin, and cytochrome c), and cells (Escherichia coli, Streptococcus thermophilus, and Saccharomyces cerevisiae). The separation results showed that under the appropriate conditions, separation efficiency was obviously better than that in the previous experiments with randomly or empirically selected conditions.

show abstract

Role of Joule heating in dispersive mixing effects in electrophoretic cells: Convective-diffusive transport aspects

Bosse¹,

Arce²

2000

Electrophoresis

View full text Add to dashboard Cite

Heat transfer design of an electrophoresis experiment

Cited by 8 publications

References 0 publications

Protein separation by continuous‐flow electrophoresis in microgravity

Protein separation by continuous‐flow electrophoresis in microgravity

Experimental study on the optimization of general conditions for a free‐flow electrophoresis device with a thermoelectric cooler†

Role of Joule heating in dispersive mixing effects in electrophoretic cells: Convective-diffusive transport aspects

Contact Info

Product

Resources

About