Utilizing ultrasonic excitation as an active method for studying the rate of heat transfer has gained considerable attention recently. The present study investigated the effects of ultrasonic excitation on the heat transfer rate in a fin-and-flat tube heat exchanger experimentally. The performance of the heat exchanger was investigated with and without the presence of ultrasonic excitation. A comprehensive parameter study was attempted, so several parameters, including ambient temperature, flow rate, air passing velocity, Reynolds number, and Nusselt number, were studied in a relatively wide range. An adequate uncertainty test, as well as a validation assessment, is provided to certify the credibility of the obtained results and the hired facility. The results revealed that reducing the flow rate, ambient temperature, and air passing velocity on the heat exchanger increased the ultrasonic excitation’s effects. The highest heat transfer enhancement in the present experiment was 70.11%, measured at the lowest air passing velocity and ambient temperature with a Reynolds number 2166. The data presented in this paper will be useful for the optimal design of ultrasonic vibrating fin-and-tube heat exchangers.