“…A peripheral lesion may not communicate with a bronchus or the bronchus may be obstructed so that the cells do not reach the trachea; the patient may be exfoliating cells but there may not be sufficient bronchial secretion to carry this away. Several methods have been devised to increase the secretions of mucus, for example, by the inhalation of aerosol solutions, usually preheated to about 160°F, of saline and propylene glycol in combination (Berkson & Snider, 1960;Umiker et al, 1960;Leilop, Garrett & Lyon, 1961;Lillehei, 1961;Rome & Olson, 1961;Olsen, Froeb & Palmer, 1961;Johnson et al, 1962;Fontana et al, 1962;Kim et al, 1963;Frenzel & Papageorgiou, 1964;Fontana, Olsen & Woolner, 1964;Fontana et al, 1965;Ozgelen et al, 1965). In a series of over 1000 cases, Fontana, Olsen & Woolner (1964), have obtained satisfactory specimens of sputum for cytology in 9900 of cases of malignant disease of the lung.…”