Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage

He, Yong; Li, Hongyan; He, Jing; Zhao, Xingxu

doi:10.1016/j.anireprosci.2014.06.019

Cited by 7 publications

(11 citation statements)

References 26 publications

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“…He–Ne laser irradiation can increase mitochondrial electrochemical potential and increase ATP synthesis. 8 The ATP content was shown to be increased after HIR in our previous study, 7 which suggested to us that HIR improves sperm-fertilizing potential by changing sperm mitochondria function as a possible mechanism. Because mitochondria play a key role in energy production and maintenance of sperm motility, 9 this conclusion has encouraged us to believe that sperm mitochondria are the key reason that HIR can enhance ram sperm motility.…”

Section: Introductionmentioning

confidence: 72%

“…We have demonstrated that HIR treatment can improve ram sperm motility after 24 hours of in vitro liquid storage 7 ; however, the mechanism underlying this phenomenon remains unclear. In the present study, we focused on the mitochondrial function combined with 2-DE to reveal the underlying mechanism that HIR treatment can improve ram sperm motility after storage at 5°C for 24 hours.…”

Section: Discussionmentioning

confidence: 93%

“…The NIR group was kept the same temperature conditions with CIR group, and all experiments were performed within 30 minutes. 7…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, we determined that the best suitable radiation dose for this purpose was 0.1 Gy. 7 There is, however, limited information available about the mechanism underlying enhanced sperm quality by HIR; thus, we need to delineate this potential mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…We also confirmed that a suitable dose of CIR has a positive effect that can promote greater ram sperm motility and raise the adenosine triphosphate (ATP) content in sperm over 24 hours of in vitro liquid storage at 15 C. Furthermore, we determined that the best suitable radiation dose for this purpose was 0.1 Gy. 7 There is, however, limited information available about the mechanism underlying enhanced sperm quality by HIR; thus, we need to delineate this potential mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage

Zhang

et al. 2019

Dose-Response

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The aim of this study was to reveal the mechanism of enhanced ram sperm motility induced by heavy ion radiation (HIR) after in vitro liquid storage. Ram semen was stored for 24 hours at 5°C and then irradiated with 0.1 Gy carbon ion radiation (CIR). In comparison to nonirradiated (NIR) sperm, the motility, viability, and adenosine triphosphate content were all higher in CIR sperm, and the reactive oxygen species levels were lower. Moreover, 87 differential mitochondrial protein spots were detected in 2-dimensional gels between CIR and NIR sperm and were identified as 52 corresponding proteins. In addition, 33 differential proteins were involved in a main pathway network, including COX5B, ERAB/HSD17B10, ETFA, SDHB, and SOD2, which are known to be involved in cell communication, energy production, and antioxidant responses. We used immunoblotting and immunofluorescence to analyze the content and localization of these proteins, respectively, and the levels of these proteins in CIR sperm were lower than those in NIR sperm. An understanding of the molecular function of these proteins could provide further insight into the mechanisms underlying high sperm motility induced by HIR in rams.

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Section: Introductionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 93%

“…The NIR group was kept the same temperature conditions with CIR group, and all experiments were performed within 30 minutes. 7…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage

Zhang

et al. 2019

Dose-Response

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Low‐dose radiation‐induced demethylation of 3β‐HSD participated in the regulation of testosterone content

Huang

Qiu

Yue³

et al. 2021

J of Applied Toxicology

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The effects of low‐dose radiation (LDR, ≤0.1 Gy) on living organisms have been the hot areas of radiation biology but do not reach a definitive conclusion yet. So far, few studies have adequately accounted for the male reproductive system responses to LDR, particularly the regulation of testosterone content. Hence, this study was designed to evaluate the effects of LDR on Leydig cells and testicular tissue, especially the ability to synthesize testosterone. We found that less than 0.2‐Gy 60Co gamma rays did not cause significant changes in the hemogram index and the body weight; also, pathological examination did not find obvious structural alterations in testis, epididymis, and other radiation‐sensitive organs. Consistently, the results from in vitro showed that only more than 0.5‐Gy gamma rays could induce remarkable DNA damage, cycle arrest, and apoptosis. Notably, LDR disturbed the contents of testosterone in mice serums and culture supernatants of TM3 cells and dose dependently increased the expression of 3β‐HSD. After cotreatment with trilostane (Tril), the inhibitor of 3β‐HSD, increased testosterone could be partially reversed. Besides, DNA damage repair‐related enzymes, including DNMT1, DNMT3B, and Sirt1, were increased in irradiated TM3 cells, accompanying by evident demethylation in the gene body of 3β‐HSD. In conclusion, our results strongly suggest that LDR could induce obvious perturbation in the synthesis of testosterone without causing organic damage, during which DNA demethylation modification of 3β‐HSD might play a crucial role and would be a potential target to prevent LDR‐induced male reproductive damage.

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Differential proteome association study of freeze-thaw damage in ram sperm

Wang

Zhao

et al. 2016

Cryobiology

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Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage

Cited by 7 publications

References 26 publications

Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage

Comparative Analysis of Mitochondrial Proteome Reveals the Mechanism of Enhanced Ram Sperm Motility Induced by Carbon Ion Radiation After In Vitro Liquid Storage

Low‐dose radiation‐induced demethylation of 3β‐HSD participated in the regulation of testosterone content

Differential proteome association study of freeze-thaw damage in ram sperm

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