Heavy metal accumulation, biochemical and transcriptomic biomarkers in earthworms Eisenia andrei exposed to industrially contaminated soils from south-eastern Tunisia (Gabes Governorate)

Hattab, Sabrine; Boughattas, Iteb; Cappello, Tiziana; Zitouni, Nesrine; Touil, Ghada; Romdhani, Ilef; Livet, Alexandre; Bousserrhine, Nourreddine; Banni, Mohamed

doi:10.1016/j.scitotenv.2023.163950

Cited by 11 publications

(4 citation statements)

References 74 publications

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“…Our data indicate significant enrichment of pathways related to genetic information processing, including “Ribosome biogenesis in eukaryotes” in both the midgut and fat body, and “ribosome” and “DNA replication” in the fat body. Exposure to heavy metals can induce DNA damage through ROS production, resulting in increased DNA attacks and reduced repair processes ( 46 ). Additionally, pathways associated with cellular processes such as “lysosome” and “apoptosis-fly” were enriched in the midgut.…”

Section: Discussionmentioning

confidence: 99%

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

Wen,

Wang,

et al. 2024

Front. Vet. Sci.

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Mercury (Hg) contamination poses a global threat to the environment, given its elevated ecotoxicity. Herein, we employed the lepidopteran model insect, silkworm (Bombyx mori), to systematically investigate the toxic effects of Hg-stress across its growth and development, histomorphology, antioxidant enzyme activities, and transcriptome responses. High doses of Hg exposure induced evident poisoning symptoms, markedly impeding the growth of silkworm larvae and escalating mortality in a dose-dependent manner. Under Hg exposure, the histomorphology of both the midgut and fat body exhibited impairments. Carboxylesterase (CarE) activity was increased in both midgut and fat body tissues responding to Hg treatment. Conversely, glutathione S-transferase (GST) levels increased in the fat body but decreased in the midgut. The transcriptomic analysis revealed that the response induced by Hg stress involved multiple metabolism processes. Significantly differently expressed genes (DEGs) exhibited strong associations with oxidative phosphorylation, nutrient metabolisms, insect hormone biosynthesis, lysosome, ribosome biogenesis in eukaryotes, and ribosome pathways in the midgut or the fat body. The findings implied that exposure to Hg might induce the oxidative stress response, attempting to compensate for impaired metabolism. Concurrently, disruptions in nutrient metabolism and insect hormone activity might hinder growth and development, leading to immune dysfunction in silkworms. These insights significantly advance our theoretical understanding of the potential mechanisms underlying Hg toxicity in invertebrate organisms.

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Section: Discussionmentioning

confidence: 99%

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

Wen,

Wang,

et al. 2024

Front. Vet. Sci.

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“…Liver tissue removes endogenous and exogenous toxins using GST in combination with reduced GSH with the electrophile center by the formation of a thioester bond between the substrate and the sulfur atom of GSH (Gulçin et al, 2018). GST plays an important role in detoxifying and metabolizing many xenobiotic and endogenous compounds (Hattab et al, 2023). Many compounds derived from plants possess antioxidant potential.…”

Section: Figurementioning

confidence: 99%

In vitro and in vivo antioxidant therapeutic evaluation of phytochemicals from different parts of Dodonaea viscosa Jacq

Khan,

Rehman,

Khan

et al. 2023

Front. Chem.

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Introduction: Natural antioxidants are vital to promote health and treat critical disease conditions in the modern healthcare system. This work adds to the index of natural medicines by exploring the antioxidant potential of Dodonaea viscosa Jacq. (Plant-DV).Material and Methods: The aqueous extract of leaves and flower-containing seeds from plant-DV in freshly prepared phosphate buffer is evaluated for antioxidant potential. In vitro antioxidant potential of the nascent and oxidatively stressed extracts was analyzed through glutathione (GSH) assay, hydrogen peroxide (H2O2) scavenging effect, glutathione-S-transferase (GST) assay, and catalase (CAT) activity. In vivo therapeutic assessment is performed in Wistar Albino rats using vitamin C as a positive control. The livers and kidneys of individual animals are probed for glutathione, glutathione-S-transferase, and catalase activities.Results: flower-containing seeds have GSH contents (59.61 µM) and leaves (32.87 µM) in the fresh aqueous extracts. The hydrogen peroxide scavenging effect of leaves is superior to flower-containing seeds with 17.25% and 14.18% respectively after 30 min incubation. However, oxidatively stressed extracts with Ag(I) and Hg(II) show declining GSH and GST levels. The plant extracts are non-toxic in rats at 5000 mg/Kg body weight. Liver and kidneys homogenate reveal an increase in GSH, GST, and CAT levels after treatment with 150 ± 2 mg/kg and 300 ± 2 mg/kg body weight plant extract compared with normal saline-treated negative and vitamin C treated positive control.Discussion: The crude aqueous extracts of leaves and flower-containing seeds of plant-DV show promising antioxidant potential both in in vitro and in vivo evaluation.

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“…Poor soil quality is defined by the presence of PTEs, and the mortality and descending reproduction rate of E. fetida. Biomarkers such as anti-oxidant defensive systems and lysosomal membrane stability are used for the quantitative analysis and identification of PTEs collectively with physicochemical analysis [18]. A decrease in lysosomal membrane stability and an increase in metallothionein content indicate Cu, Mn, Zn, and Cd metal accumulation in soil environments [18].…”

Section: Soil Environmentmentioning

confidence: 99%

“…Biomarkers such as anti-oxidant defensive systems and lysosomal membrane stability are used for the quantitative analysis and identification of PTEs collectively with physicochemical analysis [18]. A decrease in lysosomal membrane stability and an increase in metallothionein content indicate Cu, Mn, Zn, and Cd metal accumulation in soil environments [18]. The structural index of the nematode community is another PTE soil contamination assessment tool that is usually used in combination with biomarkers such as phytochelatin.…”

Section: Soil Environmentmentioning

confidence: 99%

An Overview of Quality Assessment Methods for Water and Soil in Mining Regions

Petropoulou,

Frousiou,

Vasileiou

2023

RawMat 2023

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Heavy metal accumulation, biochemical and transcriptomic biomarkers in earthworms Eisenia andrei exposed to industrially contaminated soils from south-eastern Tunisia (Gabes Governorate)

Cited by 11 publications

References 74 publications

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

In vitro and in vivo antioxidant therapeutic evaluation of phytochemicals from different parts of Dodonaea viscosa Jacq

An Overview of Quality Assessment Methods for Water and Soil in Mining Regions

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