Elements with density higher than 5 g cm À3 have been called heavy metals. From the metallic elements that naturally exist, 53 elements belong to the heavy metal group. Some of these heavy metals, such as iron, manganese, copper, zinc, and molybdenum, are known as micronutrients in agriculture, since their amount in soil is low and the little quantity of them is necessary for plant growth. Another group of these elements, such as cobalt and vanadium, has been shown to be useful for plant growth. The other category, such as nickel, chromium, selenium, lead, and cadmium, has toxic effects on plant and other organisms even in low level. The mobility and uptake of these elements and the appearing of their negative effect in the environment depend on soil, water, and plant relations.The term "chelating agents" refers to ligands occupying multiple positions in the inner coordination of a metal ion. Heavy metals combine with one or more reactive groups or ligands like oxygen (-OH, -COO, -POO), nitrogen (-NH 2 , -NH), or sulfur (-SH, -S-S). The result of this phenomenon is the formation of soluble multidentate metal-chelant complexes called "chelates" (Fig. 20.1) which came from a Greek word "chele" meaning the claw of a crab or scorpion (Tsang et al. 2012).To know the increase of metal solubility in soil, it is important to figure out the metal ion-ligand interactions. The complex formation can be considered as equilibrium reaction between the ligand and the metal ion: