Heavy Metal Effects on the Biodegradation of Fluorene by Sphingobacterium sp. KM-02 in liquid medium

Nam, In-Hyun; Kim, Jae-Gon; Chon, Chul-Min

doi:10.7857/jsge.2012.17.6.082

Cited by 3 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…또한, 토양 내 존재하는 중금속들은 대부분 토양 입자에 흡착되어 이온 형태로 산화 및 환원되어 환경에 따라 비교적 자유롭게 거동을 하므로 토착 미생물의 활동 을 저해하기도 한다 (Baath, 1989;Nies, 1999;Mittal and Ratra, 2000;Amor et al, 2001;Sokhn et al, 2001;Nam et al, 2012). 미생물을 활용하여 토양으로부 터 중금속을 제거하는 메커니즘은 세포 내로의 금속 이온 의 전달을 수반하여 더 많은 양의 중금속을 축적할 수 있는 대사 의존형과, 세포의 세포벽, 고분자물질 등에 의 해 중금속과 방사성물질이 표면 흡착을 하여 흡착 속도가 빠른 대사 비의존형 등 두 가지로 분류 할 수 있다 (Gadd, 1992).…”

unclassified

Effects of Heavy Metal and pH on Bacterial Growth Isolated from the Contaminated Smelter Soil

Keum¹,

Yoon²,

Nam³

2015

Journal of Soil and Groundwater Environment

Self Cite

View full text Add to dashboard Cite

The contaminated soil at abandoned smelter areas present challenge for remediation, as the degraded materials are typically deficient in nutrients, and rich in toxic heavy metals and metalloids. Bioremediation technique is to isolate new strains of microorganisms and develop successful protocols for reducing metal toxicity with heavy metal tolerant species. The present study collected metal contaminated soil and characterized for pH and EC values, and heavy metal contents. The pH value was 5.80, representing slightly acidic soil, and EC value was 13.47 mS/m. ICP-AES analytical results showed that the collected soil samples were highly contaminated with various heavy metals and metalloids such as lead (183.0 mg/kg), copper (98.6 mg/kg), zinc (91.6 mg/kg), and arsenic (48.1 mg/kg), respectively. In this study, a bacterial strain, Bacillus cereus KM-15, capable of adsorbing the heavy metals was isolated from the contaminated soils by selective enrichment and characterized to apply for the bioremediation. The effects of heavy metal on the growth of the Bacillus cereus KM-15 was determined in liquid cultures. The results showed that 100 mg/L arsenic, lead, and zinc did not affect the growth of KM-15, while the bacterial growth was strongly inhibited by copper at the same concentration. Further, the ability of the bacteria to adsorb heavy metals was evaluated.

show abstract

unclassified

Effects of Heavy Metal and pH on Bacterial Growth Isolated from the Contaminated Smelter Soil

Keum¹,

Yoon²,

Nam³

2015

Journal of Soil and Groundwater Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…일 반적으로 박테리아 urease는 200~250 kD의 분자량을 나타 낸다 (Min, 2001 (Chung, 1998;Muynck et. al., 2010 (Jung and Jung, 2006;Kim, 2010;Nam et al, 2012). 따라서, 본 연구에서 분리한 Urease 활…”

unclassified

Characterization of Urease-Producing Bacteria Isolated from Heavy Metal Contaminated Mine Soil

Park¹,

Yoon²,

Nam³

2014

Korean Journal of Soil Science and Fertilizer

Self Cite

View full text Add to dashboard Cite

Acid mine drainage occurrence is a serious environmental problem by mining industry; it usually contain high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of greatest concern. It causes mine impacted soil pollution with mining and smelting activities, fossil fuel combustion, and waste disposal. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a mineimpacted area. All isolated bacterial strains were identified Sporosarcina pasteurii with more than 98% of similarity, therefore they were named Sporosarcina sp. KM-01, KM-07, and KM-12. The heavy metals detected from the collected mine soils containing bacterial isolates as Mn (170.50 mg kg -1 ), As (114.05 mg kg -1 ), Zn (92.07 mg kg -1 ), Cu (62.44 mg kg -1 ), and Pb (40.29 mg kg -1 ). The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS analyses showed that calcium carbonate was successfully produced and increased with time. To confirm the calcium carbonate precipitation ability, urease activity and precipitate weight were also measured and compared. These results demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals by mining activity.

show abstract

Bioremediation of Heavy Metal Contaminated Mine Wastes using Urease Based Plant Extract

Roh¹,

Park²,

Chon³

et al. 2015

Journal of Soil and Groundwater Environment

View full text Add to dashboard Cite

Acid mine drainage occurrence is a serious environmental problem by mining industry, it usually contains high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of the greatest concern. An indigenous plant extract was used to produce calcium carbonate from Canavalia ensiformis as effective biomaterial, and its ability to form the calcium carbonate under stable conditions was compared to that of purified urease. X-ray diffraction and scanning electron microscopy were employed to elucidate the mechanism of calcium carbonate formation from the crude plant extracts. The results revealed that urease in the plant extracts catalyzed the hydrolysis of urea in liquid state cultures and decreased heavy metal amounts in the contaminated soil. The heavy metal amounts were decreased in the leachate from the treated mine soil; 31.7% of As, 65.8% of Mn, 50.6% of Zn, 51.6% of Pb, 45.1% of Cr, and 49.7% of Cu, respectively. The procedure described herein is a simple and beneficial method of calcium carbonate biomineralization without cultivation of microorganisms or further purification of crude extracts. This study suggests that crude plant extracts of Canavalia ensiformis have the potential to be used in place of purified forms of the enzyme during remediation of heavy metal contaminated soil.

show abstract

Heavy Metal Effects on the Biodegradation of Fluorene by Sphingobacterium sp. KM-02 in liquid medium

Cited by 3 publications

References 35 publications

Effects of Heavy Metal and pH on Bacterial Growth Isolated from the Contaminated Smelter Soil

Effects of Heavy Metal and pH on Bacterial Growth Isolated from the Contaminated Smelter Soil

Characterization of Urease-Producing Bacteria Isolated from Heavy Metal Contaminated Mine Soil

Bioremediation of Heavy Metal Contaminated Mine Wastes using Urease Based Plant Extract

Contact Info

Product

Resources

About