Heavy metal-induced lipogenic gene aberration, lipid dysregulation and obesogenic effect: a review

Zhou, Yang; Addai, Frank Peprah; Zhang, Xinshuang; Liu, Yuelin; Wang, Yinfeng; Lin, Feng; Tuffour, Alex; Gu, Jie; Liu, Guangxiang; Shi, Haifeng

doi:10.1007/s10311-021-01383-9

Cited by 12 publications

(3 citation statements)

References 190 publications

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“…Metal ions play an important role in the biological function of most enzymes including as a cofactor for enhanced catalytic activity and structural stabilization or to facilitate enzyme–substrate binding through coordination links. , The influence of some metal ions (Ca 2+ , Cd 2+ , Co 2+ , Cu 2+ , Fe 2+ , Hg 2+ , Mg 2+ , Mn 2+ , and Ni 2+ ) on the activity of the free lipase was first studied at concentrations of 1, 5, and 10 mM by incubating the enzyme–metal mixture at room temperature for 30 min. The reaction was carried out with tributyrin as a substrate at 45 °C for 20 min.…”

Section: Resultsmentioning

confidence: 99%

Alloyed Trimetallic Nanocomposite as an Efficient and Recyclable Solid Matrix for Ideonella sakaiensis Lipase Immobilization

Addai,

Wu,

Lin

et al. 2024

Langmuir

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In this work, a trimetallic (Ni/Co/Zn) organic framework (tMOF), synthesized by a solvothermal method, was calcinated at 400 and 600 °C and the final products were used as a support for lipase immobilization. The material annealed at 400 °C (Ni-Co-Zn@400) had an improved surface area (66.01 m2/g) and pore volume (0.194 cm3/g), which showed the highest enzyme loading capacity (301 mg/g) with a specific activity of 0.196 U/mg, and could protect the enzyme against thermal denaturation at 65 °C. The optimal pH and temperature for the lipase were 8.0 and 45 °C but could tolerate pH levels 7.0–8.0 and temperatures 40–60 °C. Moreover, the immobilized enzyme (Ni-Co-Zn@Lipase, Ni-Co-Zn@400@Lipase, or Ni-Co-Zn@600@Lipase) could be recovered and reused for over seven cycles maintaining 80, 90, and 11% of its original activity and maintained a residual activity >90% after 40 storage days. The remarkable thermostability and storage stability of the immobilized lipase suggest that the rigid structure of the support acted as a protective shield against denaturation, while the improved pH tolerance toward the alkaline range indicates a shift in the ionization state attributed to unequal partitioning of hydroxyl and hydrogen ions within the microenvironment of the active site, suggesting that acidic residues may have been involved in forming an enzyme–support bond. The high enzyme loading capacity, specific activity, encouraging stability, and high recoverability of the tMOF@Lipase indicate that a multimetallic MOF could be a better platform for efficient enzyme immobilization.

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Section: Resultsmentioning

confidence: 99%

Alloyed Trimetallic Nanocomposite as an Efficient and Recyclable Solid Matrix for Ideonella sakaiensis Lipase Immobilization

Addai,

Wu,

Lin

et al. 2024

Langmuir

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“…Studies in recent years have identified high levels of lysosomal b-Galactosidases expression in tumours and have been targeted as potential biomarker for the early detection of tumours. Cellular senescence is an irreversible stable state of cell cycle arrest caused by various macromolecular changes induced by internal and external stimuli (Park et al 2021;Zhou et al 2022b). The primary triggering factors that can result in the early initiation of cellular senescence include reactive oxygen species (ROS), DNA damage and oncogene activation, etc.…”

Section: Biomarker For Tumorigenesismentioning

confidence: 99%

“…2021; Zhou et al . 2022b). The primary triggering factors that can result in the early initiation of cellular senescence include reactive oxygen species (ROS), DNA damage and oncogene activation, etc.…”

Section: Catalytic Applications Of β‐Galactosidasesmentioning

confidence: 99%

β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine

Zhou,

Liu,

Gao

et al. 2024

Int J of Dairy Tech

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β‐Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β‐1,4‐glycosidic bonds. Though the traditional application of β‐Galactosidases has been to catalyse the breakdown of lactose in dairy products, its application extends beyond the production of lactose‐free products since variants capable of facilitating lactose condensation and exhibiting galactosyl transferase activity are extensively utilised for the synthesis of prebiotic galacto‐oligosaccharides. This review analyses β‐Galactosidase in multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering and applications in terms of whey treatment, biofuel production, production of lactose‐free dietary product, synthesis of galacto‐oligosaccharides and the early detection of cellular senescence and tumours.

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Enhancing the Purification and Stability of Superoxide Dismutase by Fusion with Thermoresponsive Self‐Assembly of Elastin Like Polypeptide

Wang,

Xia

et al. 2024

ChemistrySelect

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Elastin‐like polypeptide (ELP) is a thermo‐sensitive biosynthetic polymer composed of a Val‐Pro‐Gly‐Xaa‐Gly repeating unit possessing a sharp reversible phase transition property at specific temperatures. Here, ELP was fused to human superoxide dismutase 1 (hSOD1) modified with His tag to produce recombinant hSOD1‐Linker‐ELP‐His (hSODLEH) which was expressed in E. coli and purified via inverse thermal cycling (ITC) and Ni‐NTA resin. The results showed that ELP tag did not affect the soluble expression of SOD1. The purification by ITC was superior to Ni‐NTA resin due to its convenient purification process, improved recovery rate and purification fold, thus indicating industrial suitability for large scale protein purification in a cost‐ and time‐efficient manner. Moreover, one round of ITC was sufficient for the recovery of highly purified recombinant SOD. The results further showed that ELP improved the stability of the SOD, and did not affect its secondary structure nor its ability to bind divalent metal ions. Overall, ELP‐protein fusion system could be a promising tool for large scale enzyme purification.

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Heavy metal-induced lipogenic gene aberration, lipid dysregulation and obesogenic effect: a review

Cited by 12 publications

References 190 publications

Alloyed Trimetallic Nanocomposite as an Efficient and Recyclable Solid Matrix for Ideonella sakaiensis Lipase Immobilization

Alloyed Trimetallic Nanocomposite as an Efficient and Recyclable Solid Matrix for Ideonella sakaiensis Lipase Immobilization

β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine

Enhancing the Purification and Stability of Superoxide Dismutase by Fusion with Thermoresponsive Self‐Assembly of Elastin Like Polypeptide

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