Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network

Monsieurs, Pieter; Moors, Hugo; Houdt, Rob Van; Janssen, Paul; Janssen, Ann; Coninx, Ilse; Mergeay, Max; Leys, Natalie

doi:10.1007/s10534-011-9473-y

Cited by 126 publications

(145 citation statements)

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“…Ten of these genes were found to be induced in response to different heavy metals [7] like the pMOL30-encoded copQ [32] and czcJ [33], while three others were induced by hydrogen peroxide (Saiful Islam Muhammed, pers. comm.).…”

Section: Resultsmentioning

confidence: 99%

“…For C. metallidurans type strain CH34 a substantial part of the metal resistance mechanisms are carried by its two megaplasmids pMOL28 and pMOL30 [5,6]. However, next to these specialized plasmids also chromosomally-encoded metal responsive gene clusters have been identified [7]. The C. metallidurans CH34 genome [8] hosts in addition a large diversity of mobile genetic elements (MGEs) including genomic islands (GIs), integrative and conjugative elements, transposons and Insertion Sequence (IS) elements [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Variation in genomic islands contribute to genome plasticity in Cupriavidus metallidurans

et al. 2012

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BackgroundDifferent Cupriavidus metallidurans strains isolated from metal-contaminated and other anthropogenic environments were genotypically and phenotypically compared with C. metallidurans type strain CH34. The latter is well-studied for its resistance to a wide range of metals, which is carried for a substantial part by its two megaplasmids pMOL28 and pMOL30.ResultsComparative genomic hybridization (CGH) indicated that the extensive arsenal of determinants involved in metal resistance was well conserved among the different C. metallidurans strains. Contrary, the mobile genetic elements identified in type strain CH34 were not present in all strains but clearly showed a pattern, although, not directly related to a particular biotope nor location (geographical). One group of strains carried almost all mobile genetic elements, while these were much less abundant in the second group. This occurrence was also reflected in their ability to degrade toluene and grow autotrophically on hydrogen gas and carbon dioxide, which are two traits linked to separate genomic islands of the Tn4371-family. In addition, the clear pattern of genomic islands distribution allowed to identify new putative genomic islands on chromosome 1 and 2 of C. metallidurans CH34.ConclusionsMetal resistance determinants are shared by all C. metallidurans strains and their occurrence is apparently irrespective of the strain's isolation type and place. Cupriavidus metallidurans strains do display substantial differences in the diversity and size of their mobile gene pool, which may be extensive in some (including the type strain) while marginal in others.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variation in genomic islands contribute to genome plasticity in Cupriavidus metallidurans

et al. 2012

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“…7,9 The main detoxification mechanism is based on efflux systems with multiple cross responses. 7,9,10 However, bacteria protected by efflux systems are able to detoxify their cytoplasm but not the environment, which represents limitations to exploit the high biotechnological potential of C. metallidurans CH34 for metal remediation. 11 Different attempts to develop a bacterial strain improved for metal bioremediation are reported in the literature.…”

Section: ■ Introductionmentioning

confidence: 99%

Synthetic Phytochelatin Surface Display in Cupriavidus metallidurans CH34 for Enhanced Metals Bioremediation

Biondo

Silva

Vicente

et al. 2012

Environ. Sci. Technol.

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This work describes the effects of the cell surface display of a synthetic phytochelatin in the highly metal tolerant bacterium Cupriavidus metallidurans CH34. The EC20sp synthetic phytochelatin gene was fused between the coding sequences of the signal peptide (SS) and of the autotransporter β-domain of the Neisseria gonorrhoeae IgA protease precursor (IgAβ), which successfully targeted the hybrid protein toward the C. metallidurans outer membrane. The expression of the SS-EC20sp-IgAβ gene fusion was driven by a modified version of the Bacillus subtilis mrgA promoter showing high level basal gene expression that is further enhanced by metal presence in C. metallidurans. The recombinant strain showed increased ability to immobilize Pb 2+ , Zn 2+ , Cu 2+ , Cd 2+ , Mn 2+ , and Ni 2+ ions from the external medium when compared to the control strain. To ensure plasmid stability and biological containment, the MOB region of the plasmid was replaced by the E. coli hok/sok coding sequence.

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“…It has been widely accepted that in the model bacterium Cupriavidus metallidurans, these mechanisms appear to be cooperative, not metal specific, and are controlled by a complex regulatory network involving several clusters of genes and functions (1,27). However, for other metal-resistant bacteria, especially some recently isolated species utilized in phytoremediation, investigations of such global regulatory metal response systems are still limited.…”

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Survival Strategies of the Plant-Associated Bacterium Enterobacter sp. Strain EG16 under Cadmium Stress

Chen

Chao

et al. 2016

Appl Environ Microbiol

113

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c Plant-associated bacteria are of great interest because of their potential use in phytoremediation. However, their ability to survive and promote plant growth in metal-polluted soils remains unclear. In this study, a soilborne Cd-resistant bacterium was isolated and identified as Enterobacter sp. strain EG16. It tolerates high external Cd concentrations (Cd 2؉ MIC, >250 mg liter ؊1 ) and is able to produce siderophores and the plant hormone indole-3-acetic acid (IAA), both of which contribute to plant growth promotion. Surface biosorption in this strain accounted for 31% of the total Cd accumulated. The potential presence of cadmium sulfide, shown by energy-dispersive X-ray (EDX) analysis, suggested intracellular Cd binding as a Cd response mechanism of the isolate. Cd exposure resulted in global regulation at the transcriptomic level, with the bacterium switching to an energyconserving mode by inhibiting energy-consuming processes while increasing the production of stress-related proteins. The stress response system included increased import of sulfur and iron, which become deficient under Cd stress, and the redirection of sulfur metabolism to the maintenance of intracellular glutathione levels in response to Cd toxicity. Increased production of siderophores, responding to Cd-induced Fe deficiency, not only is involved in the Cd stress response systems of EG16 but may also play an important role in promoting plant growth as well as alleviating the Cd-induced inhibition of IAA production. The newly isolated strain EG16 may be a suitable candidate for microbially assisted phytoremediation due to its high resistance to Cd and its Cd-induced siderophore production, which is likely to contribute to plant growth promotion. Heavy metal contamination has become one of the most serious environmental problems in recent years. Metal extraction activities are major sources for heavy metals in the environment (1). Cadmium is highly toxic to plants, animals, microorganisms, and humans even at quite low concentrations (2, 3). Although Cd is non-redox active, it can cause oxidative stress by generating reactive oxygen species (ROS) (4), which can lead to DNA damage (5), inhibit the DNA mismatch repair system (6), and disrupt the synthesis of nucleic acids and proteins (7).Phytoremediation, a low-cost and eco-friendly technology for the decontamination of heavy-metal-polluted soils, uses plants to absorb, accumulate, and detoxify heavy metals in soil (3). However, the serious environmental stresses present in most cases of phytoremediation result in slow plant growth, low biomass production, and long time frames for remediation, limiting the usefulness of this technology (8). Beneficial plant-associated bacteria have been shown to protect plants from metal toxicity and promote plant growth. The plant growth-promoting (PGP) characteristics that most of these bacteria possess, including N 2 fixation, siderophore production, the production of plant growth hormones, such as indole-3-acetic acid (IAA), and the reduction of ethylen...

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Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network

Cited by 126 publications

References 65 publications

Variation in genomic islands contribute to genome plasticity in Cupriavidus metallidurans

Variation in genomic islands contribute to genome plasticity in Cupriavidus metallidurans

Synthetic Phytochelatin Surface Display in Cupriavidus metallidurans CH34 for Enhanced Metals Bioremediation

Survival Strategies of the Plant-Associated Bacterium Enterobacter sp. Strain EG16 under Cadmium Stress

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