Heavy Metal Tolerance and Antibiotic Resistance of Bacillus spp. Isolated from Two Major Rivers in Bangladesh

Shammi, Tahmina; Ahmed, Sangita

doi:10.3329/bjm.v30i1-2.28448

Cited by 10 publications

(7 citation statements)

References 9 publications

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“…and Pseudomonas sp. are the most commonly reported bacteria in soil, and possess much higher tolerance to heavy metals and other substances that are ever present in the environment [10,[24][25][26][27][28]. Dinucleotide repeats of eight bases (GCG CGC GC) were observed in all Bacillus sp.…”

Section: Molecular Identification Of Bacteriamentioning

confidence: 99%

“…The combined expression of antibiotic and heavy-metal resistance by B. cereus and P. aeruginosa may not be a chance phenomenon but rather a result of selection by heavy metal present in an environment [26,36,43]. Therefore, pragmatic measures must be taken to limit the spread of antimicrobial resistance across the environment and to reduce the incidence of healthcareassociated infections.…”

Section: Antibiotics Sensitivity and Resistance Pattern Of Isolated Bmentioning

confidence: 99%

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Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India

Nath¹,

Paul²,

Roy³

et al. 2019

SN Appl. Sci.

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The present study aims to assess the physicochemical properties and prevalence of microbial communities in soils samples collected from different locations of Cachar district, Assam, India. Bacterial communities in the soil were screened by morphological, biochemical and 16S rDNA sequence analysis and were identified as Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and Chromobacterium pseudoviolaceum. High concentrations of toxic metals negatively affect bacterial growth, and therefore, the minimum inhibitory concentration of isolated bacteria was determined against Cd, Pb, Fe and Cu by agar dilution technique. Co-resistance of antibiotic was also determined, which demonstrated that most of the metal-tolerant isolates were resistant to Methicillin and Penicillin. However, P. aeruginosa showed resistance to other antibiotics such as Cefdinir, Ampicillin, Kanamycin, Rifampicin and Vancomycin. The development and evolution of antibiotic resistance in soil bacteria occurs very likely naturally as a result of unethical and non-scientific disposal of toxic substances and industrial discharge, which also includes heavy-metal effluents and other clinical by-products. Therefore, pragmatic measures must be taken to limit the spread of antimicrobial resistance across the environment and to reduce the incidence of healthcare-associated infections.

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Section: Molecular Identification Of Bacteriamentioning

confidence: 99%

Section: Antibiotics Sensitivity and Resistance Pattern Of Isolated Bmentioning

confidence: 99%

Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India

Nath¹,

Paul²,

Roy³

et al. 2019

SN Appl. Sci.

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“…As shown in Figure 5, the phylogenetic tree based on 16S rRNA gene sequences confirms that the iron-tolerant isolates fell into two genera; Bacillus and Staphylococcus. It seems that because the genus Bacillus is ubiquitous in terrestrial areas and is extensively distributed in nature, Bacillus species are dominant bacteria in heavy metal-polluted areas (Zampieri et al 2016;Shammi 2016;Alboghobeish et al 2014). Also, the ability of Staphylococcus species to tolerate heavy metals has been confirmed in other research (Feßler et al 2017).…”

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth mentioning

confidence: 58%

“…In addition, the results reveal that all iron tolerant isolates tested for antibiotic susceptibility had multiple antibiotic resistances ( Table 2). Association of antibiotic resistance with metal tolerance is common and well documented in many other studies (Gandhi et al 2015;Neethu et al 2015;Shammi and Ahmed 2016).This could be as a result of genes for metal tolerance and antibiotic resistance genes existing together on the same R-plasmid and or transposons in bacteria. Closely associated genes would likely be distributed together in the environment (Shammi and Ahmed, 2016;Gandhi et al 2015).…”

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth mentioning

confidence: 97%

Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan

et al. 2017

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Abstract. Alnaimat S, Shattal SA, Althunibat O, Alsbou E, Amasha R. 2017. Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan. Biodiversitas 18: 1250-1257. This study deals with enumeration, identification and molecular characterization of bacterial species tolerant of iron (II) and other heavy metals that isolated from rock varnish collected from the arid region of Al-Jafer Basin, Jordan. Based on conventional culture-dependent methods, six isolates exhibited a high degree of tolerance to iron (II) and other heavy metals with a minimum inhibitory concentration (550-600 mg/L) to iron, (500-550 mg/L) to lead, (350 mg/L) to copper, (400-450 mg/L) to chromium, (150-200 mg/L) to silver, and (150-200 mg/L) to mercury, in solid media. The results showed that all isolates could tolerate 2000 mg/L Fe 2+ expressed as maximum tolerance concentration (MTC). The multiple metal resistances of these isolates were also associated with multiple antibiotic resistances. These iron tolerant isolates were shown to be capable of growth at temperatures ranging from 30 to 40°C and pH values ranging from 5 to 9 with some differences between the six isolates. On the basis of morphology and of 16S rDNA gene sequencing and phylogeny analysis, the iron-tolerant isolates were identified as Bacillus zhangzhouensis (DVCr1), Staphylococcus warneri (DVCr6), Bacillus cereus (DVCr7), Staphylococcus pasteuri (DVCu2), Brevibacterium frigoritolerans (DVPb) and Bacillus altitudinis (DVCr10). The 16S rDNA sequences from five of the six strains were submitted to GenBank and are available under the accession numbers KX781139, KX781140, KX781141, KX781145 and KX781148. The results highlight the high potential of a non-polluted habitat like rock varnish to provide new microbial communities that could be used for enhanced bioremediation of heavy metals from a contaminated site.

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“…Several studies suggest that antibiotic and heavy metal resistance genes are often encoded together on the same plasmid 13,14 . Therefore, the heavy metal tolerant Lactobacillus isolates were tested for their antibiotic resistance profile.…”

mentioning

confidence: 99%

Probiotic Lactobacillus sp. with bioremediation potential of toxic heavy metals

Ahmed

Islam

Ferdousi

et al. 2018

Bangla. J. Microbiol.

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Pollution of the environment and food with toxic heavy metals is being intensified in Bangladesh with industrial development. Consumption of foods and water contaminated with heavy metals imposes threat to human health. Aiming to find a solution to this problem, the present study focuses on probiotic Lactobacillus spp. with potential to remove heavy metals from environment as well as human body. A total of three Lactobacillus spp were isolated from curd samples and were identified based on their morphological and biochemical properties. These isolates were tolerant to low pH and bile salt which aids in their application in human gut. All isolates could tolerate 600 ppm chromium, 400 ppm lead, 400 ppm copper and 400 ppm zinc. The heavy metal tolerant Lactobacillus spp were also multi drug resistant and showed 100% resistance to Azithromycin, Cloxacillin, Gentamicin, Vancomycin, Streptomycin, Nalidixic acid, Trimethoprim-Sulfamethoxazole and Penicillin, while 100% sensitivity was observed to Imipenem. Bangladesh J Microbiol, Volume 34 Number 1 June 2017, pp 43-46

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Heavy Metal Tolerance and Antibiotic Resistance of Bacillus spp. Isolated from Two Major Rivers in Bangladesh

Cited by 10 publications

References 9 publications

Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India

Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India

Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan

Probiotic Lactobacillus sp. with bioremediation potential of toxic heavy metals

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