Heberden Oration

Neuberger, A.

doi:10.1136/ard.19.1.1

Cited by 15 publications

(1 citation statement)

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“…Neuberger [9] suggested that the source of all the enzymes which cause breakdown of connective tissue in rheumatic diseases is the fibroblast of connective tissue. However, to date no lysosomal prep arations of connective tissue other than bone [12] have been made.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Rat Liver, Spleen and Skin Lysosomes

Iqbal¹,

Ch²

1970

Enzymol Biol Clin

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Four acid hydrolytic enzymes, acid ß-glycerophosphatase, acid phenylphosphatase, ß-glucuronidase and cathepsin, were found to be present in 0.25 M sucrose homogenates of new-born rat skin. When skin homogenates were fractionated these enzymes were found to occur partly in soluble and partly in particulate fractions. Particulate-bound enzymes could be released by hypo-osmotic shock, freezing and thawing and Triton x-100. A comparative study of liver, spleen and skin lysosomes in tissue-homogenates and isolated fractions showed that at pH 5.2 and 0° all lysosomes were quite stable for 3 h. At 37°, the pattern of release of enzymes from homogenates was in all cases the same as that from isolated lysosomal fractions. Tissue homogenates were slightly more stable than the isolated fractions, and this is considered to be due to denser membrane packing in the presence of other cytoplasmic particles. Spleen lysosomes were more stable than those of liver, and skin lysosomes were far more stable than those of either liver or spleen. When treated with high concentrations of vitamin A, liver and spleen lysosomes were very unstable, but skin lysosomes were little affected.

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Section: Introductionmentioning

confidence: 99%