Hedgehog pathway as a therapeutic target for gallbladder cancer. Immunohistochemical staining for Gli1 in gallbladder cancer.

Matsushita, Shojiro; Onishi, Hideya; Nakano, Kenji; Nagamatsu, Iori; Imaizumi, Akira; Hattori, Masami; Oda, Yoshinao; Tanaka, Masao

doi:10.1111/cas.12357

Cited by 2 publications

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“…The Shh signaling pathway is a major regulator of various processes, including cell differentiation and proliferation, and tissue polarity [ 2 , 5 ]. Inhibition of Shh signaling is widely researched because aberrant Shh signaling is a hallmark of many cancers [ 6 , 7 , 8 ], including prostate, gallbladder, pancreatic, and basal cell carcinoma [ 9 , 10 , 11 , 12 ]. Basal cell carcinoma (BCC) is the most common human cancer and is driven predominantly by the hyperactivation of the Hh pathway [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Native V. californicum Alkaloid Combinations Induce Differential Inhibition of Sonic Hedgehog Signaling

et al. 2018

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Veratrum californicum is a rich source of steroidal alkaloids such as cyclopamine, a known inhibitor of the Hedgehog (Hh) signaling pathway. Here we provide a detailed analysis of the alkaloid composition of V. californicum by plant part through quantitative analysis of cyclopamine, veratramine, muldamine and isorubijervine in the leaf, stem and root/rhizome of the plant. To determine whether additional alkaloids in the extracts contribute to Hh signaling inhibition, the concentrations of these four alkaloids present in extracts were replicated using commercially available standards, followed by comparison of extracts to alkaloid standard mixtures for inhibition of Hh signaling using Shh-Light II cells. Alkaloid combinations enhanced Hh signaling pathway antagonism compared to cyclopamine alone, and significant differences were observed in the Hh pathway inhibition between the stem and root/rhizome extracts and their corresponding alkaloid standard mixtures, indicating that additional alkaloids present in these extracts are capable of inhibiting Hh signaling.

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Section: Introductionmentioning

confidence: 99%

Native V. californicum Alkaloid Combinations Induce Differential Inhibition of Sonic Hedgehog Signaling

et al. 2018

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“…Inhibition of Shh signalling is widely researched because aberrant Shh signalling is a hallmark of many cancers [6][7][8]. This has been reported in many cancers, including prostate, gallbladder, pancreatic, and basal cell carcinoma [9][10][11][12]. Basal cell carcinoma (BCC) is the most common human cancer and is driven predominantly by the hyper activation of the Hh pathway [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Native <em>V. Californicum</em> Alkaloid Combinations Induce Differential Inhibition of Sonic Hedgehog Signaling<strong> </strong>

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Cruz

Elwell

et al. 2018

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Veratrum californicum is a rich source of steroidal alkaloids such as cyclopamine, a known inhibitor of the Hedgehog (Hh) signaling pathway. Here we provide a detailed analysis of the alkaloid composition of V. californicum by plant part through quantitative analysis of cyclopamine, veratramine, muldamine and isorubijervine in the leaf, stem and root/rhizome of the plant. To determine if additional alkaloids in the extracts contribute to Hh signaling inhibition, we replicated the concentrations of these alkaloids observed in extracts using commercially available standards and compared the inhibitory potential of the extracts to alkaloid standard mixtures using Shh-Light II cells. Alkaloid combinations enhanced Hh signaling pathway antagonism compared to cyclopamine alone, and significant differences were observed in the Hh pathway inhibition between the stem and root/rhizome extracts and their corresponding alkaloid standard mixtures, indicating that additional alkaloids present in these extracts contribute to Hh signaling inhibition.

show abstract

Hedgehog pathway as a therapeutic target for gallbladder cancer. Immunohistochemical staining for Gli1 in gallbladder cancer.

Cited by 2 publications

References 0 publications

Native V. californicum Alkaloid Combinations Induce Differential Inhibition of Sonic Hedgehog Signaling

Native V. californicum Alkaloid Combinations Induce Differential Inhibition of Sonic Hedgehog Signaling

Native <em>V. Californicum</em> Alkaloid Combinations Induce Differential Inhibition of Sonic Hedgehog Signaling<strong> </strong>

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