Hedgehog Signaling Acts with the Temporal Cascade to Promote Neuroblast Cell Cycle Exit

Chai, Phing Chian; Zhong, Lei; Chia, William; Cai, Yu

doi:10.1371/journal.pbio.1001494

Cited by 48 publications

(74 citation statements)

References 93 publications

(103 reference statements)

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“…Biochemical studies have not only shown that PTPA reactivates the serine/threonine phosphatase activity of inactive PP2A, but also that it increases peptidylprolyl cis-trans isomerase activity (Chao et al, 2006). Consistently, the yeast PTPA homologs Ypa1 and Ypa2 can physically bind to the yeast PP2A homologs Pph21 and Pph22.…”

Section: Introductionmentioning

confidence: 90%

“…The following fly stocks were used in this study: wor-gal4, Df(2L)BSC163, Df(2L)BSC164, Df(2R)BSC359, mts XE-2258 (Betschinger et al, 2003), UAS-mira::3GFP (gift from C. Petritsch, UCSF). MACRM clones were generated as previously described (Chai et al, 2013). For GFP-labeled Ptpa 1 mutant clones, we used an elav-gal4,hs-FLP;UAS-nlacZ,UAS-mCD8::GFP;P[FRT]40A, Fig.…”

Section: Fly Strainsmentioning

confidence: 99%

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Phosphotyrosyl phosphatase activator facilitates Miranda localization through dephosphorylation in dividing neuroblasts

et al. 2015

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The mechanism for the basal targeting of the Miranda (Mira) complex during the asymmetric division of Drosophila neuroblasts (NBs) is yet to be fully understood. We have identified conserved Phosphotyrosyl phosphatase activator (PTPA) as a novel mediator for the basal localization of the Mira complex in larval brain NBs. In mutant Ptpa NBs, Mira remains cytoplasmic during early mitosis and its basal localization is delayed until anaphase. Detailed analyses indicate that PTPA acts independent of and before aPKC to localize Mira. Mechanistically, our data show that the phosphorylation status of the T591 residue determines the subcellular localization of Mira and that PTPA facilitates the dephosphorylation of T591. Furthermore, PTPA associates with the Protein phosphatase 4 complex to mediate localization of Mira. On the basis of these results, a two-step process for the basal localization of Mira during NB division is revealed: cortical association of Mira mediated by the PTPA-PP4 complex is followed by apical aPKC-mediated basal restriction.

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Section: Introductionmentioning

confidence: 90%

Section: Fly Strainsmentioning

confidence: 99%

Phosphotyrosyl phosphatase activator facilitates Miranda localization through dephosphorylation in dividing neuroblasts

et al. 2015

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“…Ecdysone acts together with the Mediator complex and Syncrip to promote neuroblast size reduction, nuclear Prospero localization, and terminal differentiation [36, 58]. This late event also requires Hedgehog signaling [59], and down-regulation of Imp [60]. The detailed mechanism of ecdysone, Mediator, and Hedgehog signaling remains to be determined.…”

Section: Hormonal Cues Regulate Larval Neuroblast Temporal Identitymentioning

confidence: 99%

Playing Well with Others: Extrinsic Cues Regulate Neural Progenitor Temporal Identity to Generate Neuronal Diversity

2017

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During neurogenesis, vertebrate and Drosophila progenitors change over time as they generate a diverse population of neurons and glia. Vertebrate neural progenitors have long been known to use both progenitor-intrinsic and progenitor-extrinsic cues to regulate temporal patterning. In contrast, virtually all temporal patterning mechanisms discovered in Drosophila neural progenitors (neuroblasts) involve progenitor-intrinsic temporal transcription factor cascades. Recent results, however, have revealed several extrinsic pathways that regulate Drosophila neuroblast temporal patterning: nutritional cues regulate the timing of neuroblast proliferation/quiescence and a steroid hormone cue that is required for temporal transcription factor expression. Here we discuss newly discovered extrinsic cues regulating neural progenitor temporal identity in Drosophila, highlight conserved mechanisms, and raise open questions for the future.

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“…As with abdominal VNC NBs, embryonic Cas induces Grh expression, and after hatching, Cas induces Hh expression in these NBs (Chai et al . ). Hh signaling acts as an autocrine and/or paracrine factor to sustain Grh expression, which in turn prevents premature nuclear localization of Pros to exit the cell cycle (Maurange et al .…”

Section: Termination Of Nb Proliferationmentioning

confidence: 97%

“…Type I and Type II NBs in the central brain and thoracic VNC NBs use similar termination machinery, where they begin to shrink following puparium formation, and most NBs exit the cell cycle at approximately 24 h after puparium formation (APF) (Figs 1C, 7B) (Ito & Hotta 1992;Maurange et al 2008;Homem et al 2014). As with abdominal VNC NBs, embryonic Cas induces Grh expression, and after hatching, Cas induces Hh expression in these NBs (Chai et al 2013). Hh signaling acts as an autocrine and/or paracrine factor to sustain Grh expression, which in turn prevents premature nuclear localization of Pros to exit ª 2015 Japanese Society of Developmental Biologists the cell cycle (Maurange et al 2008).…”

Section: Termination Of Nb Proliferationmentioning

confidence: 99%

Temporal regulation of the generation of neuronal diversity in Drosophila

Yasugi¹,

Nishimura²

2015

Dev Growth Differ

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For the construction of complex neural networks, the generation of neurons and glia must be tightly regulated both spatially and temporally. One of the major issues in neural development is the generation of a large variety of neurons and glia over time from a relatively small number of neural stem cells. In Drosophila, neural stem cells, called neuroblasts (NBs), have been used as a useful model system to uncover the molecular and cellular machinery involved in the establishment of neural diversity. NBs divide asymmetrically and produce another self-renewing progenitor cell and a differentiating cell. NBs are subdivided into several types based on their location in the central nervous system. Each type of NB has specific features related to the timing of cell generation, cell cycle progression, temporal patterning for neuronal specification, and termination mechanism. In this review, we focus on the molecular mechanisms that regulate the proliferation of NBs and generate a large variety of neuronal and glia subtypes during development.

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Hedgehog Signaling Acts with the Temporal Cascade to Promote Neuroblast Cell Cycle Exit

Abstract: During the development of the Drosophila nervous system, the developmentally regulated Hedgehog pathway, together with a series of temporal transcription factors, schedules the end of neurogenesis.

Cited by 48 publications

References 93 publications

Phosphotyrosyl phosphatase activator facilitates Miranda localization through dephosphorylation in dividing neuroblasts

Phosphotyrosyl phosphatase activator facilitates Miranda localization through dephosphorylation in dividing neuroblasts

Playing Well with Others: Extrinsic Cues Regulate Neural Progenitor Temporal Identity to Generate Neuronal Diversity

Temporal regulation of the generation of neuronal diversity in Drosophila

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