Hedgehog signaling pathway mediates tongue tumorigenesis in wild-type mice but not in Gal3-deficient mice

Santos, Daniela Meneses; Loyola, Adriano Mota; Cardoso, Sérgio Vitorino; Chammas, Roger; Liu, Fu‐Tong; Faria, Paulo Rogério de

doi:10.1016/j.yexmp.2014.09.018

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…In the combined model, in contrast to simple 4NQO induction, specific gene deletion or overexpression can be studied; compared with simple GEMMs, more specific OSCC can be induced, and genetic changes that are not directly carcinogenic can be investigated. This model can be used to study the role of miRNA (199,200), proteins (82, 184,203), and pathways (201,202) involved in the carcinogenesis of OSCC. Furthermore, the knock-in of green fluorescent protein can be used to label cells with specific gene changes (158,199).…”

Section: Nqo-induced Combined With Gemmsmentioning

confidence: 99%

Mouse Tumor-Bearing Models as Preclinical Study Platforms for Oral Squamous Cell Carcinoma

Dong

Yang

et al. 2020

Front. Oncol.

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Preclinical animal models of oral squamous cell carcinoma (OSCC) have been extensively studied in recent years. Investigating the pathogenesis and potential therapeutic strategies of OSCC is required to further progress in this field, and a suitable research animal model that reflects the intricacies of cancer biology is crucial. Of the animal models established for the study of cancers, mouse tumor-bearing models are among the most popular and widely deployed for their high fertility, low cost, and molecular and physiological similarity to humans, as well as the ease of rearing experimental mice. Currently, the different methods of establishing OSCC mouse models can be divided into three categories: chemical carcinogen-induced, transplanted and genetically engineered mouse models. Each of these methods has unique advantages and limitations, and the appropriate application of these techniques in OSCC research deserves our attention. Therefore, this review comprehensively investigates and summarizes the tumorigenesis mechanisms, characteristics, establishment methods, and current applications of OSCC mouse models in published papers. The objective of this review is to provide foundations and considerations for choosing suitable model establishment methods to study the relevant pathogenesis, early diagnosis, and clinical treatment of OSCC.

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Section: Nqo-induced Combined With Gemmsmentioning

confidence: 99%

Mouse Tumor-Bearing Models as Preclinical Study Platforms for Oral Squamous Cell Carcinoma

Dong

Yang

et al. 2020

Front. Oncol.

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“…Por outro lado, poucos trabalhos utilizam camundongos, mas estudos com esses animais indicam que nessa espécie de roedores é também possível induzir lesões displásicas e carcinomas (IDE et al, 2001;TANG et al, 2004;FARIA et al, 2011;DE SOUZA et al, 2014;DE OLIVEIRA SANTOS et al, 2014).…”

Section: Carcinoma De Células Escamosas Bucal Experimentalunclassified

“…Tabela 6: Associação entre as variáveis estudadas e a imuno-marcação de iNOS em amostras de carcinoma de células escamosas bucal. Nossos resultados mostram ainda uma mortalidade animal próxima a 20%, resultado semelhante a nossas experiências anteriores com o modelo (FARIA et al, 2011;DE SOUZA et al, 2014;DE OLIVEIRA SANTOS et al, 2014) (CASTELLANI, 1985). Seu potencial carcinogênico é bem definido, mas não existem atualmente guias disponíveis sobre como tais resíduos devem ser tratados para minimizar seu potencial contaminante e carcinogênico, e por consequência, reduzir a possibilidade de agressão ao meio ambiente e aos pesquisadores expostos na sua manipulação.…”

Section: Associação Da a Expressão Da Proteína Inos E Nitrotirosina unclassified

“…Estas modificações foram graduadas como leves, moderadas e intensas, servindo também como guia para o monitoramento do processo. Todavia, nossos dados confirmam o que outros grupos vêm observando em outros trabalhos(FARIA et al, 2011; DE SOUZA et al, 2014;DE OLIVEIRA SANTOS et al, 2014). A propósito,Hawkins et al, 1994, mostraram que a mucosa exposta ao 4NQO pode apresentar uma aparência normal mesmo na presença de um carcinoma invasivo situado profundamente no tecido.…”

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Efeitos da enzima óxido nítrico sintase induzível (iNOS) no desenvolvimento e progressão do carcinoma de células escamosas bucal experimental e humano

Servato¹

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Upregulation of PTEN suppresses invasion in Tca8113 tongue cancer cells through repression of epithelial–mesenchymal transition (EMT)

Xie

Lin

et al. 2015

Tumor Biol.

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We previously discovered that the expression of the tumor suppressor phosphatase and tensin homolog (PTEN) was downregulated in the majority patients with tongue squamous cell carcinoma (TSCC). The aim of this study was to investigate the role of PTEN overexpression in the regulation of epithelial-mesenchymal transition (EMT) of the tongue squamous carcinoma cell line Tca8113 as well as explore the underlying mechanism. GV230 (containing the PTEN gene) and empty vectors were transfected into Tca8113 cells. After stable transfection, the messenger RNA (mRNA) and protein levels of PTEN were validated using quantitative real-time PCR (qPCR) and Western blot analysis. The growth and cell cycle were analyzed using Cell Counting Kit-8 (CCK-8) and flow cytometry, respectively. The invasion ability was measured with a transwell assay. The effects of PTEN overexpression on EMT and Hedgehog signaling were assessed by comparing Tca8113-PTEN cells with control and negative control cell groups. We found that PTEN expression was significantly upregulated after transfection. Meanwhile, upregulated PTEN inhibited the proliferation and invasion of Tca8113 cells. In addition, we observed changes in the EMT- and Hedgehog-associated proteins. These data demonstrated that PTEN upregulation could reduce invasion by inhibiting the process of EMT in Tca8113 cells, which might be related to the Hedgehog signaling pathway.

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Hedgehog signaling pathway mediates tongue tumorigenesis in wild-type mice but not in Gal3-deficient mice

Cited by 5 publications

References 39 publications

Mouse Tumor-Bearing Models as Preclinical Study Platforms for Oral Squamous Cell Carcinoma

Mouse Tumor-Bearing Models as Preclinical Study Platforms for Oral Squamous Cell Carcinoma

Efeitos da enzima óxido nítrico sintase induzível (iNOS) no desenvolvimento e progressão do carcinoma de células escamosas bucal experimental e humano

Upregulation of PTEN suppresses invasion in Tca8113 tongue cancer cells through repression of epithelial–mesenchymal transition (EMT)

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