Heightened Lung Bactericidal Activity in Mice after Aerosol Immunization with Re 595 Salmonella minnesota: Importance of Cellular rather than Humoral Factors

LaForce, F. Marc; Boose, Dorothy; Mills, David

doi:10.1093/infdis/142.3.421

Cited by 17 publications

(9 citation statements)

References 22 publications

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“…It seems safe to assume that this increase is the result of immunostimulation as has been previously reported. La Force, using bronchoalveolar lavage in mice after aerosol immunization with acid-hydrolyzed Re 595 Salmonella minnesota, reported an enhancement of cells and, particularly, of polymorphonuclear cells (20,22). Though it was not seen in our study, an increase in neutrophils has been observed after inhalation of lipopolysaccharides of gram-negative bacteria (12, 36).…”

Section: Discussionmentioning

confidence: 39%

“…Our observations of IgAbearing cells in the lamina propria and submucous glands confirm an endogenous production of IgA but do not rule out an exogenous production. Though it was not done, it would have been interesting to measure antibody levels in serum to know whether or not the local response induces a systemic response; no correlation between aerosol immunization and systemic immune status or bactericidal activity has ever been observed in any of the animal models studied until now (14,15,20,22).…”

Section: Figmentioning

confidence: 99%

“…For technical reasons that will be explained below, fiberscopic biopsies do not provide adequate experimental material. This lack of material has been a major obstacle to the extensive study of aerosol immunization, which has been shown to be more effective than the systemic route in enhancing the defense mechanisms of the respiratory membrane in animals (14,16,22,33).…”

mentioning

confidence: 99%

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Appearance of specific antibody-bearing cells in human bronchial mucosa after local immunization with bacterial vaccine

Latil¹,

Vervloet²,

Casanova³

et al. 1986

J Clin Microbiol

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The immune response to local in vivo inhalation of a lysed bacteria vaccine was assessed in surgical specimens of main-stem bronchi from patients who had undergone pneumectomy for cancer. The patient population included 22 subjects; 11 of these received the aerosol vaccine twice a day for 10 days prior to surgery, while the remaining 11 patients were used as controls and were not immunized. The submucous glands of immunized subjects showed significantly more cells than did those of the controls, i.e., 62 +/- 8 versus 37 +/- 7, respectively (P less than 0.05). The following five antigens were chosen for study by fluorescence assay: Streptococcus pneumoniae types II and III, Haemophilus influenzae, Streptococcus sp. strain D19, and Klebsiella pneumoniae. An immunization-dependent correlation was found between immunoglobulin A, immunoglobulin A-bearing cells, and specific antibody-bearing cells on the one hand and three of the five antigens (S. pneumoniae types II and III and Streptococcus sp. strain D19) on the other hand. This is the first time that a relationship has been established between bacterial immunization of the lower respiratory tract and local immunoglobulin production in humans.

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Section: Discussionmentioning

confidence: 39%

Section: Figmentioning

confidence: 99%

mentioning

confidence: 99%

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Appearance of specific antibody-bearing cells in human bronchial mucosa after local immunization with bacterial vaccine

Latil¹,

Vervloet²,

Casanova³

et al. 1986

J Clin Microbiol

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“…Regardless of the mechanism responsible for this paradoxical effect of inoculum size on pulmonary clearance, however, these results clearly indicate that systemic immunization enhanced lower respiratory tract defenses against Hib, resulting in enhanced pulmonary clearance of this pathogen. Previous studies have suggested that immunization may enhance lung bactericidal activity by increasing the number of PMNs in the alveolar spaces (18,19). In the present study no significant differences were found in the total number of phagocytic cells or PMNs at any time point when immunized animals were compared to control animals (Table 4).…”

Section: Discussionmentioning

confidence: 99%

Effect of systemic immunization on pulmonary clearance of Haemophilus influenzae type b

Toews¹,

Hart²,

Hansen³

1985

Infect Immun

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The effect of systemic immunization on pulmonary clearance of Haemophilus influenzae type b (Hib) was studied in a mouse model system. Immunization of mice by intraperitoneal injection of viable Hib cells resulted in the appearance of Hib-directed antibodies in both serum and bronchoalveolar lavage fluid. The development of this Hib-directed antibody activity was associated with significant enhancement of early pulmonary clearance of Hib. Systemic immunization did not affect the recruitment of polymorphonuclear leukocytes to the alveoli, suggesting that the enhanced clearance of Hib observed in immunized animals was due to specific antibodies which promote either phagocytosis or extracellular killing of Hib. The spectrum of Hib-directed antibody specificities detected in sera from immunized animals was essentially identical to that detected in bronchoalveolar lavage fluids from these same animals. Similarly, intravenous administration of an immunoglobulin G monoclonal antibody specific for Hib lipopolysaccharide resulted in the subsequent appearance of this antibody in the alveolar spaces where it enhanced pulmonary clearance of Hib. This study shows that this mouse model system can be used to measure the effect of both active and passive immunization on the clearance of Hib from the lower respiratory tract.

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“…marcescens occurs normally in the natural environment and in the human intestinal tract, and sometimes causes opportunistic infection in the human. Since phagocytosis by polymorphonuclear leukocytes (PMN) is considered to be responsible for the defense mechanism of host animals against this bacterium [16][17][18], the effects of ornithine lipid and a surface lipid molecule from S. marcescens on phagocytosis and phagosomelysosome fusion in human PMN were examined.…”

Section: Introductionmentioning

confidence: 99%

Stimulation and inhibition of polymorphonuclear leukocytes phagocytosis by lipoamino acids isolated from Serratia marcescens

Miyazaki

1993

FEMS Immunology and Medical Microbiology

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We reconfirmed that the LD50s of hemolytic Enterococcus faecalis strains were significantly less than those of nonhemolytic E. faecalis strains in normal mice. Hemolysin produced by E. faecalis lysed human, horse, rabbit, and mouse erythrocytes, but not cow and sheep erythrocytes. Sphingomyelin comprises a part of the lipid composition of the erythrocyte membrane of all mammalian species tested. But phosphatidylcholine exists only in human, horse, rabbit, and mouse. These two lipids inhibited lysis of horse erythrocytes by hemolytic E. faecalis. Phosphatidylcholine is probably the binding component on the membrane of erythrocytes for E. faecalis hemolysin. The hemolytic culture supernatant lysed not only erythrocytes but also mouse polymorphonuclear neutrophils (PMNs) and macrophages.

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Heightened Lung Bactericidal Activity in Mice after Aerosol Immunization with Re 595 Salmonella minnesota: Importance of Cellular rather than Humoral Factors

Cited by 17 publications

References 22 publications

Appearance of specific antibody-bearing cells in human bronchial mucosa after local immunization with bacterial vaccine

Appearance of specific antibody-bearing cells in human bronchial mucosa after local immunization with bacterial vaccine

Effect of systemic immunization on pulmonary clearance of Haemophilus influenzae type b

Stimulation and inhibition of polymorphonuclear leukocytes phagocytosis by lipoamino acids isolated from Serratia marcescens

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