We report the coexistence of the Kondo effect and spin glass behavior in Fe-doped NbS 2 single crystals. The Fe x NbS 2 shows the resistance minimum and negative magnetoresistance due to the Kondo effect, and exhibits no superconducting behavior at low temperatures. The resistance curve follows a numerical renormalization-group theory using the Kondo temperature T K = 12.3 K for x = 0.01 as evidence of Kondo effect. Scanning tunneling microscope/spectroscopy (STM/STS) revealed the presence of Fe atoms near sulfur atoms and asymmetric spectra. The magnetic susceptibility exhibits a feature of spin glass. The static critical exponents determined by the universal scaling of the nonlinear part of the susceptibility suggest a three-dimensional Heisenberg spin glass. The doped-Fe atoms in the intra-and inter-layers revealed by the X-ray result can realize the coexistence of the Kondo effect and spin glass.