Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype

Stanley, John R.; Linton, Dennis; Burnens, André P.; Dewhirst, Floyd E.; Owen, Robert J.; Porter, Anthony; On, Stephen L. W.; Costas, M.

doi:10.1099/00221287-139-10-2495

Cited by 146 publications

(104 citation statements)

References 36 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…Until now, more than 25 species of Helicobacter have been found, and these microorganisms have been confirmed to be causative agents in various diseases of the stomach, intestine, and the liver in human and mammals. [25][26][27][28][29] Hepatitis and hepatomas are caused by H. hepaticus in mice, 27,29 and H. pulorum, Flexispira rappini, and H. canis are isolated in diarrheal patients, showing the possibility of zoonosis. 25,26,30,31 This strongly suggests that Helicobacter may be a triggering agent in liver diseases as well.…”

Section: Discussionmentioning

confidence: 99%

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Lee

et al. 2010

Gut Liver

View full text Add to dashboard Cite

Background/Aims: Bacterial infection is accepted as a precipitating factor in cholesterol gallstone formation, and recent studies have revealed the presence of Helicobacter species in the hepatobiliary system. We utilized the polymerase chain reaction (PCR) to establish the presence of bacterial DNA, including from Helicobacter species, in gallstones, bile juice, and gallbladder mucosa from patients with gallstones. Methods: At cholecystectomy, 58 gallstones, 48 bile samples, and 46 gallbladder mucosa specimens were obtained and subjected to nested PCR using specific 16S rRNA primers of H. pylori and other bacteria. Bacterial species were identified by DNA sequencing analysis. Bacterial 16S rRNA was detected in 25 out of 36 mixed-cholesterol gallstones, 1 out of 10 purecholesterol gallstones, and 9 out of 12 pigmented stones. Furthermore, 16S rDNA sequencing identified Escherichia coli, Pseudomonas, Citrobacter, Klebsiella, and Helicobacter species. Results: Helicobacter DNA was detected in 4 out of 58 gallstones, 6 out of 48 bile samples, and 5 out of 46 gallbladder specimens. Direct sequencing of Helicobacter amplicons confirmed strains of H. pylori in all four gallstones, five out of six bile samples, and three out of five gallbladder specimens. Almost all mixed-cholesterol gallstones appear to harbor bacterial DNA, predominantly E. coli. Conclusions: H. pylori was also found in the biliary system, suggesting that these bacteria are of etiological importance in gallstone formation. (Gut Liver 2010; 4:60-67)

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Lee

et al. 2010

Gut Liver

View full text Add to dashboard Cite

show abstract

“…All strains were obtained from the Division of Comparative Medicine culture collection at Massachusetts Institute of Technology: H. hepaticus 3B1 (ATCC51449) [6], H. bilis (ATCC51630 T ) [8], H. canis (ATCC51401 T ) [36], H. pylori (NCTC11639) and C. jejuni 81-176 [37]. Helicobacter sp.…”

Section: Bacterial Strains and Cultivationmentioning

confidence: 99%

Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.

Chien

Taylor

et al. 2000

Journal of Medical Microbiology

110

View full text Add to dashboard Cite

A bacterial toxin that causes progressive distension and death of Chinese hamster ovary (CHO) cells and HeLa cells, termed cytolethal distending toxin (Cdt), has been identi®ed in several diarrhoeagenic bacteria, including Campylobacter spp. (C. jejuni and C. coli), some pathogenic strains of Escherichia coli and Shigella spp. Genes encoding this toxin were identi®ed as a cluster of three adjacent genes cdtA, cdtB and cdtC. Homologues of cdtB from ®ve species of enterohepatic helicobacters ( Helicobacter hepaticus, H. bilis, H. canis and two novel Helicobacter spp. isolated from mice and woodchuck, respectively) were identi®ed by means of degenerative PCR primers, cloned and sequenced. The similarities of these partial cdtB nucleotide sequences from these Helicobacter spp. to those of cdtB genes known to be present in other bacteria were: C. jejuni, 58.3±64.8%; E. coli, 52.3±57.8%, Haemophilus ducreyi, 53.4±58.4% and Actinobacillus actinomycetemcomitans, 52.7±58.1%. Bacterial lysates from four of these helicobacters caused characteristic cytolethal distension of HeLa cells. Cdt caused cell cycle arrest at G 2 /M phase as measured by¯ow cytometry. The results demonstrated the presence of a toxin in these Helicobacter spp. belonging to the family of Cdt.

show abstract

“…For example, partial 16S rRNA sequences have been used in a number of phylogenetic studies of the Campylobacter group of organisms (21,30,42,50,54,58) and in the development of rapid identification methodology (48,49,63). The 16S rRNA genes of C. jejuni, C. coli, and a number of other campylobacters have also been sequenced (7,9,55,63). The sequence of a 5S rRNA gene of C. jejuni has also been reported (30).…”

mentioning

confidence: 99%

Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp

et al. 1994

View full text Add to dashboard Cite

The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. coli numbering) in the form of an extra sequence block approximately 147 bp long. PCR analysis of 31 other strains of C. coli and C. jejuni showed that 69%o carried a transcribed spacer of either ca. 147 or ca. 37 bp.Comparison of all sequenced Campylobacter transcribed spacers showed that the Campylobacter inserts were related in sequence and percent G+C content. All Campylobacter strains carrying transcribed spacers in their 23S rRNA genes produced fragmented 23S rRNAs. Other strains which produced unfragmented 23S rRNAs did not appear to carry transcribed spacers at this position in their 23S rRNA genes. At the 1850 region (E. coli numbering), Campylobacter 23S rRNA displayed a base pairing signature most like that of the beta and gamma subdivisions of the class Proteobacteria, but in the 270 region, Campylobacter 23S rRNA displayed a helix signature which distinguished it from the alpha, beta, and gamma subdivisions. Phylogenetic analysis comparing C. coli VC167 23S rRNA and a C.jejuni TGH9011 (ATCC 43431) 23S rRNA with 53 other completely sequenced (eu)bacterial 23S rRNAs showed that the two campylobacters form a sister group to the alpha, beta, and gamma proteobacterial 23S rRNAs, a positioning consistent with the idea that the genus Campylobacter belongs to the epsilon subdivision of the class Proteobacteria.The members of the Campylobacter group of organisms are important pathogens in human and veterinary medicine. This group includes Campylobacter, Arcobacter, Helicobacter, Wolinella, and "Flexispira" spp. and has been argued to constitute a separate bacterial lineage within the class Proteobacteria (62, 63). The most recently published phylogenetic tree based on small-subunit rRNA sequences has the group positioned in the delta and epsilon subdivisions of the Proteobacteria (purple bacteria) (39). There are 11 Campylobacter species (9, 56), and the roles of several of these species in diseases of humans and animals have been well described (43). Typically, these small, nutritionally fastidious, microaerophilic, gram-negative spiral bacteria colonize moist body surfaces, including the human gingival cavity, the small intestinal mucosa, and the vaginal mucosa of bovines and this colonization leads to disease. The two most important human pathogens in the genus are Campylobacterjejuni and C. coli, which cause enteritis.In keeping with their small size and limited nutritional properties, the campylobacters have a small genome (23,37,57). At 1.7 to 1.8 Mb, the C. jejuni and C. coli genomes are only 36% of the size of the Escherichia coli chromosome (53) (23,24,27,37,57). Unlike the characteristic continuous 16S-23S (eu)bacterial rRNA operon structure seen in E. coli (35), several studies have sug...

show abstract

Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype

Cited by 146 publications

References 36 publications

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.

Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp

Contact Info

Product

Resources

About