Helicobacter pylori Antibiotic Resistance: Molecular Basis and Diagnostic Methods

Medakina, Irina; Tsapkova, Larisa; Polyakova, Vera; Nikolaev, Sergey; Yanova, Tatyana; Dekhnich, Natalia; Khatkov, Igor; Bordin, Dmitry; Bodunova, Natalia

doi:10.3390/ijms24119433

Cited by 19 publications

(13 citation statements)

References 62 publications

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“…Drug resistance in H . pylori has been studied based on molecular [ 35 ] and genetic levels [ 36 ] but has not been effectively addressed. To assist prevent the spread of antibiotic resistance, we therefore also explored the option of supplementing CPC with antibiotics for the therapy of H .…”

Section: Discussionmentioning

confidence: 99%

In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride

Xun,

Feng,

et al. 2024

PLoS ONE

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The primary treatment method for eradicating Helicobacter pylori (H. pylori) infection involves the use of antibiotic-based therapies. Due to the growing antibiotic resistance of H. pylori, there has been a surge of interest in exploring alternative therapies. Cetylpyridinium chloride (CPC) is a water-soluble and nonvolatile quaternary ammonium compound with exceptional broad-spectrum antibacterial properties. To date, there is no documented or described specific antibacterial action of CPC against H. pylori. Therefore, this study aimed to explore the in vitro activity of CPC against H. pylori and its potential antibacterial mechanism. CPC exhibited significant in vitro activity against H. pylori, with MICs ranging from 0.16 to 0.62 μg/mL and MBCs ranging from 0.31 to 1.24 μg/mL. CPC could result in morphological and physiological modifications in H. pylori, leading to the suppression of virulence and adherence genes expression, including flaA, flaB, babB, alpA, alpB, ureE, and ureF, and inhibition of urease activity. CPC has demonstrated in vitro activity against H. pylori by inhibiting its growth, inducing damage to the bacterial structure, reducing virulence and adherence factors expression, and inhibiting urease activity.

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Section: Discussionmentioning

confidence: 99%

In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride

Xun,

Feng,

et al. 2024

PLoS ONE

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“…Além disso, a resistência antibiótica está associada a uma maior probabilidade de efeitos adversos durante o tratamento. Estudos como o de Medakina et al, (2023) relatam que o uso prolongado de antibióticos pode causar efeitos colaterais gastrointestinais, como náuseas, diarreia e distúrbios da microbiota intestinal. Esses efeitos adversos podem impactar a adesão do paciente ao tratamento e influenciar na eficácia global do regime terapêutico.…”

Section: Efeitos Adversos E Resistência Antibióticaunclassified

Tratamento Da Úlcera Gástrica Relacionada Ao Helicobacter Pylori: Abordagens Cirúrgicas E Conservadoras - Uma Revisão Bibliográfica

Mainardo Rodrigues Bezerra,

Leite de Sousa,

Silva Macedo

et al. 2024

RECIMA21

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A úlcera gástrica relacionada ao Helicobacter pylori é uma condição clínica significativa, marcada por desafios como resistência antibiótica e complicações. Abordagens terapêuticas cirúrgicas e conservadoras desempenham um papel crucial no manejo dessa condição.Objetivo: Esta revisão bibliográfica busca abordar abordagens cirúrgicas e conservadoras no tratamento da úlcera gástrica relacionada ao Helicobacter pylori. Métodos: Foi realizada uma revisão bibliográfica utilizando bases de dados científicas, como PubMed, Web of Science, Scopus e Google Scholar, no período de 2004 a 2024. Foram incluídos estudos originais e revisões que investigaram abordagens terapêuticas, fisiopatologia e qualidade de vida relacionada à úlcera gástrica e H. pylori. Resultados e Discussão: Os resultados e discussão enfatizam a eficácia das abordagens terapêuticas conservadoras, como regimes triplos, e os benefícios das cirúrgicas, como gastrectomia laparoscópica. Também são abordados desafios como resistência antibiótica, complicações e adesão ao tratamento, ressaltando a importância de uma abordagem multidisciplinar. Conclusão: Uma abordagem integrada e baseada em evidências é fundamental para otimizar o manejo da úlcera gástrica relacionada ao H. pylori. São destacadas áreas de pesquisa futuras, como terapias personalizadas e estratégias para superar desafios como resistência antibiótica e complicações, visando melhorar os resultados clínicos e a qualidade de vida dos pacientes.

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“…The disc diffusion method offers a cut-off dependent on the diameter of the zone of inhibition. This method is widely used in epidemiological investigations, but its application in ordinary day-to-day practice has been difficult and time consuming due to tedious preparations, the length of time required, and the fact that it is not cost-effective [ 23 , 34 , 35 ]. Due to the difficulty of culturing H. pylori in broth without defibrinated blood, the broth dilution method is rarely employed [ 36 ].…”

Section: Detection Of Antibiotic Resistancementioning

confidence: 99%

Drug-Resistant Helicobacter pylori: Diagnosis and Evidence-Based Approach

Jearth,

Rath,

Chatterjee

et al. 2023

Diagnostics

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Helicobacter pylori (H. pylori) is the most common chronic bacterial infection, affecting approximately half of the world’s population. H. pylori is a Class I carcinogen according to the World Health Organization, and the International Agency for Research on Cancer (IARC) has linked it to 90% of stomach cancer cases worldwide. The overall pattern points to a yearly reduction in eradication rates of H. pylori with the likelihood of success further decreasing after each unsuccessful therapeutic effort. Antimicrobial resistance in Helicobacter pylori is a major public health concern and is a predominant cause attributed to eradication failure. As a result, determining H. pylori’s antibiotic susceptibility prior to the administration of eradication regimens becomes increasingly critical. Detecting H. pylori and its antimicrobial resistance has traditionally been accomplished by time-consuming culture and phenotypic drug susceptibility testing. The resistance of H. pylori to different antibiotics is caused by various molecular mechanisms, and advances in sequencing technology have greatly facilitated the testing of antibiotic susceptibility to H. pylori. This review will summarize H. pylori antibiotic resistance patterns, mechanisms, and clinical implications. We will also review the pros and cons of current antibiotic susceptibility testing methods. Along with a comparison of tailored susceptibility-guided regimens and empirical therapy based on the latest evidence, an evidence-based approach to such situations will be explored.

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Helicobacter pylori Antibiotic Resistance: Molecular Basis and Diagnostic Methods

Cited by 19 publications

References 62 publications

In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride

In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride

Tratamento Da Úlcera Gástrica Relacionada Ao Helicobacter Pylori: Abordagens Cirúrgicas E Conservadoras - Uma Revisão Bibliográfica

Drug-Resistant Helicobacter pylori: Diagnosis and Evidence-Based Approach

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