Helicobacter pylori Chaperone-Like Protein CagT Plays an Essential Role in the Translocation of CagA into Host Cells

Ding, Honglei; Zeng, Hongxin; Huang, Linping; Dong, Yandong; Duan, Yijun; Mao, Xuhu; Guo, Gang; Zou, Quanming

doi:10.4014/jmb.1202.02025

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…In addition, the abundances of proteins of the T4SS (CagE and CagV) also increased in coccoid ( Supplementary Table S2 ). CagT, which is an essential structural component of the cag PAI apparatus, acts as a chaperone-like protein to promote the translocation of CagA, a bacterial oncoprotein, into host epithelial cells 45 . Consistent with our finding, antibiotic-induced coccoid forms of two clinical isolates has also been demonstrated to express higher rate of cagE mRNA than their spiral counterparts 46 .…”

Section: Resultsmentioning

confidence: 99%

Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach

Loke

Vilashni

et al. 2016

Sci Rep

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Helicobacter pylori may reside in the human stomach as two morphological forms: the culturable spiral form and the viable but non-culturable (VBNC) coccoid form. This bacterium transforms from spiral to coccoid under in vitro suboptimal conditions. However, both spiral and coccoid have demonstrated its infectivity in laboratory animals, suggesting that coccoid may potentially be involved in the transmission of H. pylori. To determine the relevance of the coccoid form in viability and infectivity, we compared the protein profiles of H. pylori coccoids obtained from prolonged (3-month-old) culture with that of 3-day-old spirals of two H. pylori standard strains using SWATH (Sequential Window Acquisition of all Theoretical mass spectra)-based approach. The protein profiles reveal that the coccoids retained basal level of metabolic proteins and also high level of proteins that participate in DNA replication, cell division and biosynthesis demonstrating that coccoids are viable. Most interestingly, these data also indicate that the H. pylori coccoids possess higher level of proteins that are involved in virulence and carcinogenesis than their spiral counterparts. Taken together, these findings have important implications in the understanding on the pathogenesis of H. pylori-induced gastroduodenal diseases, as well as the probable transmission mode of this bacterium.

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Section: Resultsmentioning

confidence: 99%

Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach

Loke

Vilashni

et al. 2016

Sci Rep

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“…5) (104, 105). Studies have shown that surface-variable CagT is required for CagA translocation and pilus biogenesis (106, 107), and also might contribute to immune evasion by H. pylori (102). By contrast, No domains are structural modules thought to serve a variety of functions relating to transport processes.…”

Section: Modulation Of Donor-target Cell Interactions By Adapted Formmentioning

confidence: 99%

The Mosaic Type IV Secretion Systems

Christie¹

2016

EcoSal Plus

132

156

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Escherichia coli and other Gram-negative and -positive bacteria employ type IV secretion systems (T4SSs) to translocate DNA and protein substrates, generally by contact-dependent mechanisms, to other cells. The T4SSs functionally encompass two major subfamilies, the conjugation systems and the effector translocators. The conjugation systems are responsible for interbacterial transfer of antibiotic resistance genes, virulence determinants, and genes encoding other traits of potential benefit to the bacterial host. The effector translocators are used by many Gram-negative pathogens for delivery of potentially hundreds of virulence proteins termed effectors to eukaryotic cells during infection. In E. coli and other species of Enterobacteriaceae, T4SSs identified to date function exclusively in conjugative DNA transfer. In these species, the plasmid-encoded systems can be classified as the P, F, and I types. The P-type systems are the simplest in terms of subunit composition and architecture, and members of this subfamily share features in common with the paradigmatic Agrobacterium tumefaciens VirB/VirD4 T4SS. This review will summarize our current knowledge of the E. coli systems and the A. tumefaciens P-type system, with emphasis on the structural diversity of the T4SSs. Ancestral P-, F-, and I-type systems were adapted throughout evolution to yield the extant effector translocators, and information about well-characterized effector translocators also is included to further illustrate the adaptive and mosaic nature of these highly versatile machines.

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“…However, in many systems, the VirB7-like lipoproteins have acquired surface-variable regions as shown for H. pylori CagT (Terradot and Waksman 2011; Backert et al 2015), or N0 domains as shown for Xanthomonas citri VirB7 and L. pneumophila DotD (Nakano et al 2010; Souza et al 2011). Surface-variable CagT is required for CagA translocation and pilus biogenesis (Ding et al 2012; Johnson et al 2014) and might also contribute to immune evasion by H. pylori (Fischer 2011). The N0 domains of VirB7 Xac and DotD Lp are envisioned to form additional rings within or at the base of the OMC of possible importance for channel gating or communication between the IMC and the OMCC (Nakano et al 2010; Souza et al 2011) (Fig.…”

Section: Function Structure and Diversification Of T4ass And T4bsssmentioning

confidence: 99%

Biological Diversity and Evolution of Type IV Secretion Systems

Christie¹,

Gómez-Valero

Buchrieser

2017

Current Topics in Microbiology and Immunology

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The bacterial type IV secretion systems (T4SSs) are a highly functionally and structurally diverse superfamily of secretion systems found in many species of gram-negative and -positive bacteria. Collectively, the T4SSs can translocate DNA and monomeric and multimeric protein substrates to a variety of bacterial and eukaryotic cell types. Detailed phylogenomics analyses have established that the T4SSs evolved from ancient conjugation machines whose original functions were to disseminate mobile DNA elements within and between bacterial species. How members of the T4SS superfamily evolved to recognize and translocate specific substrate repertoires to prokaryotic or eukaryotic target cells is a fascinating question from evolutionary, biological, and structural perspectives. In this chapter, we will summarize recent findings that have shaped our current view of the biological diversity of the T4SSs. We focus mainly on two subtypes, designated as the types IVA (T4ASS) and IVB (T4BSS) systems that respectively are represented by the paradigmatic Agrobacterium tumefaciens VirB/VirD4 and Legionella pneumophila Dot/Icm T4SSs. We present current information about the composition and architectures of these representative systems. We also describe how these and a few related T4ASS and T4BSS members evolved as specialized nanomachines through acquisition of novel domains or subunits, a process that ultimately generated extensive genetic and structural mosaicism among this secretion superfamily. Finally, we present new phylogenomics information establishing that the T4BSSs are much more broadly distributed than initially envisioned.

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Helicobacter pylori Chaperone-Like Protein CagT Plays an Essential Role in the Translocation of CagA into Host Cells

Cited by 11 publications

References 23 publications

Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach

Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach

The Mosaic Type IV Secretion Systems

Biological Diversity and Evolution of Type IV Secretion Systems

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