Helicobacter pylori flagellins have very low intrinsic activity to stimulate human gastric epithelial cells via TLR5

Lee, Sae Kyung; Stack, Allison; Katzowitsch, Elena; Aizawa, Shin‐Ichi; Suerbaum, Sebastian; Josenhans, Christine

doi:10.1016/j.micinf.2003.09.018

Cited by 171 publications

(132 citation statements)

References 57 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…The robustness of the proinflammatory cellular program initiated by bacterial flagellin proteins varies; flagellins from different bacteria differentially activate the TLR5-dependent signaling cascade [22,23]. The FliC and FljB Salmonella flagellin proteins display 78% sequence identity at the deduced amino acid level (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, the assembly process results in an apparent functional shielding of the TLR5 recognition sequences, thereby decreasing recognition by TLR5 [14,17,21]. Although bacterial flagellin interaction with TLR5 is an important strategy of pathogen recognition by the host immune system, examples occur whereby changes in the flagellin sequence circumvent recognition by TLR5 but still retain bacterial motor activity [22,23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activation of NF-κB-dependent gene expression by Salmonella flagellins FliC and FljB

Simon

Samuel

2007

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Bacterial flagellin is recognized by Toll-like receptor (TLR5) and activates NF-κB which leads to the induction of proinflammatory gene expression. Salmonella expresses two flagellin proteins, FliC and FljB. We purified FliC and FljB and examined the ability of the Salmonella flagellins to activate the NF-κB transcription factor in human embryonic kidney cells. We found that FliC and FljB as purified proteins possessed a comparable specific activity for activation of NF-κB-dependent gene expression in HEK293 cells. We also determined the ability of UV-inactivated bacteria, both wildtype and fliC and fljB mutant strains, to activate NF-κB. Wild-type fliC + /fljB + Salmonella and the fliC + /fljB − mutant strain were robust activators, whereas the fliC − /fljB + and flhC − mutant strains were very poor activators. The NF-κB activation capacity of bacterial strains correlated with their flagellin expression level. Finally, Salmonella cell wall-associated polymeric flagellin displayed greatly reduced ability to activate NF-κB compared to purified monomeric flagellin.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Activation of NF-κB-dependent gene expression by Salmonella flagellins FliC and FljB

Simon

Samuel

2007

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…Several studies have shown that H. pylori LPS is more than 100-fold-less potent than Escherichia coli LPS (37,48). In addition, two studies have reported that H. pylori flagellins have a low intrinsic ability to stimulate human gastric epithelial cells via TLR5 (18,36). Thus, DC activation and maturation by H. pylori seem to be more complex than simple activation of individual TLRs by bacterial components.…”

mentioning

confidence: 99%

Impact of Helicobacter pylori Virulence Factors and Compounds on Activation and Maturation of Human Dendritic Cells

et al. 2005

View full text Add to dashboard Cite

Recently, we and others have shown that Helicobacter pylori induces dendritic cell (DC) activation and maturation. However, the impact of virulence factors on the interplay between DCs and H. pylori remains elusive. Therefore, we investigated the contribution of cag pathogenicity island (PAI) and VacA status on cytokine release and up-regulation of costimulatory molecules in H. pylori-treated DCs. In addition, to characterize the stimulatory capacity of H. pylori compounds in more detail, we studied the effect of formalininactivated and sonicated H.

show abstract

“…Consequently, epithelial detection of flagellated bacteria in colon relies on bacteria able to deliver flagellin to the basolateral surface (transepithelial transport), i.e. residues 89-96 of S. typhimurium molecule, thereby impairing TLR5 activation [17,49,50]. Molecular changes within LPS have also been described, resulting in TLR ignorance or hyporesponsivness [14,51].…”

Section: Detection Of Pathogenic Motile Bacteria In the Gutmentioning

confidence: 99%

Mucosal interplay among commensal and pathogenic bacteria: Lessons from flagellin and Toll‐like receptor 5

Rumbo

Nempont

Kraehenbuhl³

et al. 2006

FEBS Letters

View full text Add to dashboard Cite

Toll‐like receptors (TLR) detect pathogen‐associated molecular patterns (PAMP) and play a crucial role in triggering immunity. Due to their large surfaces in direct contact with the environment, mucosal tissues are the major sites of PAMP‐TLR signalling. How innate and adaptive immunity are triggered through flagellin–TLR5 interaction is the main focus of the review. In view of recent reports on genetic polymorphism, we will summarize the impact of TLR5 on the susceptibility to mucosal infections and on various immuno‐pathologies. Finally, the contribution of TLRs in the induction and maintenance of mucosal homeostasis and commensal discrimination is discussed.

show abstract

Helicobacter pylori flagellins have very low intrinsic activity to stimulate human gastric epithelial cells via TLR5

Cited by 171 publications

References 57 publications

Activation of NF-κB-dependent gene expression by Salmonella flagellins FliC and FljB

Activation of NF-κB-dependent gene expression by Salmonella flagellins FliC and FljB

Impact of Helicobacter pylori Virulence Factors and Compounds on Activation and Maturation of Human Dendritic Cells

Mucosal interplay among commensal and pathogenic bacteria: Lessons from flagellin and Toll‐like receptor 5

Contact Info

Product

Resources

About