Helicobacter pylori Genotypes in Israeli Children: The Significance of Geography

Benenson, Shmuel; Halle, David; Rudensky, Bernard; Faber, J.; Schlesinger, Yechiel; Branski, David; Rabinowitz, Nina; Wilschanski, Michael

doi:10.1097/00005176-200211000-00018

Cited by 29 publications

(51 citation statements)

References 29 publications

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“…This result was different from previously reported prevalence of cagA (66-73%) in Europe but in agreement with the majority of studies conducted in Middle Eastern countries (i.e., Turkey, Egypt, Israel and Jordan) where the cagA genotype was reported to vary between 26% and 44% [35][36][37][38][39]. In a similar manner, the prevalence rates of the vacA m2 (82.9%), m1 (17.1%), and s2 (58.5%) genotypes detected in Cyprus were in agreement with those reported in other Middle Eastern countries (Table 6) [35][36][37][38][39] rather than those reported for Europe [12,13,[40][41][42]. The similarities in terms of the genotypes and their prevalence identified in the present study and what has been reported in Middle Eastern countries could be attributed to a geographic influence important in the adaptation of H. pylori to the environment and climate conditions.…”

Section: Discussioncontrasting

confidence: 85%

“…The similarities in terms of the genotypes and their prevalence identified in the present study and what has been reported in Middle Eastern countries could be attributed to a geographic influence important in the adaptation of H. pylori to the environment and climate conditions. The close resemblance of strains in neighboring countries was also reported in Israel, Egypt, and Jordan [37][38][39], as well as in Germany, France, and Poland [42,43]. The prevalence of the vacA s1 allele has been reported to range between 46% and100% in the Middle East [35], 48% and 89% in Europe [12,13,[40][41][42], and 57% and 68% in America [14,44,45].…”

Section: Discussionmentioning

confidence: 75%

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Prevalence of Helicobacter pylori cagA and vacA genes in Cypriot patients

Krashias

Bashiardes

Potamitou³

et al. 2013

J Infect Dev Ctries

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Introduction: The prevalence of H. pylori varies with geographic locations. To date there are no epidemiological data on its prevalence in Cyprus; therefore, we determined the prevalence and molecular characteristics of H. pylori infection in Cypriot patients. Methodology: DNA extracted from 103 gastric biopsies was analyzed for the presence of H. pylori by PCR using primers for ureA. H. pyloripositive biopsies were characterized by PCR using specific primers for cagA and vacA genes. The presence of clarithromycin-associated resistant mutations such as A2143G, A2142G, A2142C in 23S rRNA gene of H. pylori-positive patients was determined using a real-time PCR allelic discrimination assay. Results: H. pylori was detected in 41 (39.8%) biopsies and, out of these, 17 (41.5%) tested positive for the cagA gene. The vacA alleles m1, m2, s1a, s1b, and s2 were detected in 7 (17.1%), 34 (82.9%), 12 (29.3%), 2 (4.9%), and 22 (53.7%) isolates, respectively. One (2.4%) biopsy was vacA s1a and s2-positive while one (2.4%) was positive for vacA s1a, s1b, and s2. Three (7.3%) biopsies were untypable for vacA s1, s1b, and s2. The majority (35; 85.4%) of strains were susceptible to clarithromycin while two (4.9%) had the A2143G mutation. Three (7.3%) had a mixture of an A2143G point mutant and susceptible strains while one (2.4%) had a mixture of an A2142G point mutant and susceptible strains. Conclusions: The distribution of the virulence factors cagA and vacA in the Cypriot strains resembled that of strains circulating in Middle Eastern countries geographically close to Cyprus.

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Section: Discussioncontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 75%

Prevalence of Helicobacter pylori cagA and vacA genes in Cypriot patients

Krashias

Bashiardes

Potamitou³

et al. 2013

J Infect Dev Ctries

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“…Our data emphasizes the significant association of the most predominant (52.5%) allele combination s2/m2 with gastritis (65.2%). This predominance goes in accordance with Benenson et al (2002) and El-Gharbawy et al (2006). This similarity in H. pylori genotypes in three neighboring countries, Egypt, Jordan, and Gaza strip, indicates a geographic influence, which was reported by Abu Amra (2010).…”

Section: Discussionsupporting

confidence: 90%

Helicobacter pylori vacA genotyping in relation to cagA status, ultra-structure of gastric mucosa and clinical outcomes in Egyptian patients

Diab¹,

Shemis²,

El-Ghannam³

et al. 2016

Afr. J. Microbiol. Res.

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Helicobactor pylori (H. pylori) has been strongly associated with gastritis, peptic ulcer and is linked to an increased risk of gastric cancer. The cytotoxin-associated gene product (cagA) and the vacuolating cytotoxin (vacA) have been implicated as two major virulence factors of H. pylori. Since there is an increasing evidence that genetic variability of H. pylori may have clinical importance, we aimed to evaluate different vacA genotypes and reveal its relationship with endoscopic and transmission electron microscopy (TEM) findings among H. pylori infected Egyptian patients. Forty H. pylori infected patients possessing vacA gene who underwent upper endoscopic examination were considered to be infected with H. pylori when rapid urease test and detection of 16S rRNA in gastric biopsy recorded positive. Both vacA and cagA genotypes were detected by polymerase chain reaction (PCR). The TEM was performed to assess the ultra-structure of the gastric mucosa. Four vacA genotypes were identified, the most prominent was the s2/m2 allele combination (52.5%) followed by s1/m1 (27.5%), s1/m2 (17.5%) and s2/m1 genotype was found just in one H. pylori strain (2.5%). There were significant correlations between vacA s2/m2 and gastritis (65.2%), and vacA s1/m1 and peptic ulceration (57%). The cagA gene was associated with 38% of vacA genotypes and 60% of which were significantly associated with vacA s1/m1 genotype with the development of severe gastritis reaching up to gastric ulcer. The TEM revealed H. pylori spiral and coccoid forms, cytoplasmic vacuolar degeneration caused by vacA, swollen mitochondria and dilated rough endoplasmic reticulum. In Egypt where prevalence of H. pylori infection is high, genotyping of H. pylori virulence factors can help to predict patients who are at a high risk of related gastroduodenal diseases. Although H. pylori with vacA s2/m2 genotype is mostly related to low level of virulent strains yet, significant crosstalk between H. pylori strains harboring both vacA s1/m1 and cagA gene provides crucial insights into virulence of high level.

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“…and the cagE gene was investigated according to Sozzi et al 22 . The presence of the vacA and iceA alleles in the biopsy samples was investigated using the primers previously described 23,24 and the PCR was conducted as proposed by Benenson et al 25 . For detection of the babA2 gene, the primers and the PCR conditions applied, were described by Sheu et al 26 .…”

Section: Detection Of Pathogenicity Genes By Pcrmentioning

confidence: 99%

cagE as a biomarker of the pathogenicity of Helicobacter pylori

Ramis

Vianna

Silva

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods: Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results: Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions: Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa.

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Helicobacter pylori Genotypes in Israeli Children: The Significance of Geography

Cited by 29 publications

References 29 publications

Prevalence of Helicobacter pylori cagA and vacA genes in Cypriot patients

Prevalence of Helicobacter pylori cagA and vacA genes in Cypriot patients

Helicobacter pylori vacA genotyping in relation to cagA status, ultra-structure of gastric mucosa and clinical outcomes in Egyptian patients

cagE as a biomarker of the pathogenicity of Helicobacter pylori

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