Helicobacter pylori in the Canadian arctic: seroprevalence and detection in community water samples

McKeown, Iris

doi:10.1016/s0002-9270(99)00268-3

Cited by 36 publications

(48 citation statements)

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“…Published data, however, consistently refute any important associations between blood group and H. pylori prevalence (16,19,21,22,24,27). Therefore, this explanation is unlikely.…”

Section: Discussionmentioning

confidence: 98%

Unexpected Decrease with Age of Helicobacter pylori Seroprevalence among Swedish Blood Donors

Sörberg¹,

Nyrén²,

Granström

2003

J Clin Microbiol

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Blood donors are often used as proxies for the general population in studies of Helicobacter pylori epidemiology. Our aim was to test if the age-specific seroprevalence rates among blood donors match with the corresponding rates in a random population sample. This descriptive study was based on sera obtained from 3,502 blood donors representing all Swedish counties and cities. An age-stratified random population sample of 1,030 from Stockholm County served as comparison. Sera were analyzed by an in-house enzyme-linked immunosorbent assay for H. pylori immunoglobulin G antibodies. In the population sample, we found the expected increase with age in the seroprevalence of H. pylori infection. This was true also among young blood donors, while the prevalence-by-age curve showed a deflection downward among blood donors who are > 50 years of age. In this age group, the probability of being seropositive was reduced by 73% (95% confidence interval [CI], 63 to 81%) relative to the population sample. Overall, the adjusted odds ratio for H. pylori seropositivity among blood donors was decreased by 43% (95% CI, 28 to 55%). Thus, it appears that blood donors who are H. pylori seropositive selectively disappear from the blood donor cohort. We speculate that H. pyloriseropositive blood donors may tolerate repeated bleedings less well than do noninfected individuals and/or that the general well-being among those who are infected may be somewhat impaired. Our unexpected observation indicates that blood donors may be less suitable as proxies for the general population in analytic studies of H. pylori infection and that the underlying cause needs further study.Helicobacter pylori is accepted as a principal cause of nonautoimmune chronic gastritis (10). Moreover, eradication of H. pylori prevents recurrence of peptic ulcers (14), and evidence for a causal role in gastric cancer is accumulating (11,17,30).H. pylori infections are mainly acquired in early childhood (13). In developing countries, the seroprevalence of the infection is higher than in developed countries, where it rises gradually with age (12,23). This latter phenomenon is considered to reflect a birth cohort effect. Hence, the higher infection prevalence in older individuals represents higher childhood infection rates in these birth cohorts rather than acquisition during adult life (1, 9).In European epidemiological studies, blood donors are often used to represent the general population since all European countries have unpaid donors (34, 36). The appropriateness of this assumption has not been rigorously tested for H. pylori seroprevalence.We took advantage of a large nationwide sample of blood donors, drawn for the purpose of investigating the seroprevalence of Borrelia burgdorferi infection in different parts of Sweden (28), to study also the age-specific seroprevalence of H. pylori infection, presuming that the blood donors were representative for the general population. We then compared the seroprevalence pattern among blood donors with that in a population contr...

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“…Published data, however, consistently refute any important associations between blood group and H. pylori prevalence (16,19,21,22,24,27). Therefore, this explanation is unlikely.…”

Section: Discussionmentioning

confidence: 98%

Unexpected Decrease with Age of Helicobacter pylori Seroprevalence among Swedish Blood Donors

Sörberg¹,

Nyrén²,

Granström

2003

J Clin Microbiol

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“…It seemed that sanitary/environmental conditions remain basic in Mataram, Lombok island, Indonesia. Some people still use the fingers when eating (Cairncross et al, 1997), consume well, pond or river water (Cairncross et al, 1997;Mckeown et al, 1999 ) and use privy/latrine type toilet (Feachem et al, 1983). Prevalence of hepatitis A, for instance, a typical waterborne infectious disease, is very high at no less than 90%, and the infection rates did not differ between the two areas (Brown et al, 1985;Hoang et al, 2008) Effects of pre-or co-infection of other organisms, including dengue, should be taken into account because the altered immune system may modify sensitivity or resistance to infection by H. pylori (Nurgalieva et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Association Between HLA-DQ Genotypes and Haplotypes vs Helicobacter pylori Infection in an Indonesian Population

Zhao¹,

Wang²,

Tanaka³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Background: Helicobacter pylori is an important gastrointestinal pathogen related to the development of not only atrophic gastritis and peptic ulcer, but also gastric cancer. Human leukocyte antigens (HLA) may play particular roles in host immune responses to bacterial antigens. This study aimed to investigate the association between HLA-DQA1 and DQB1 genotypes and haplotypes vs H. pylori infection in an Indonesian population. Methods: We selected 294 healthy participants in Mataram, Lombok Island, Indonesia. H. pylori infection was determined by urea breath test (UBT). We analyzed HLA-DQA1 and DQB1 genotypes by PCR-RFLP and constructed haplotypes of HLA-DQA1 and DQB1 genes. Multiple comparisons were conducted according to the Bonferroni method. Results: The H. pylori infection rate was 11.2% in this Indonesian population. The DQB1*0401 genotype was noted to be associated with a high risk of H. pylori infection, compared with the DQB1*0301 genotype. None of the HLA-DQA1 or DQB1 haplotypes were related to the risk of H. pylori infection. Conclusions: The study suggests that HLADQB1 genes play important roles in H. pylori infection, but there was no statistically significant association between HLA-DQA1 or DQB1 haplotypes and H.pylori infection in our Lombok Indonesian population.

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“…Following gentle mixing the sample was kept at Ϫ80°C for 30 min and then centrifuged at 13,000 rpm for 15 min at 4°C. The pellet was air dried, resuspended in 30 l of sterile water, and used as a template for PCR (16).…”

Section: Samplingmentioning

confidence: 99%

Detection of Arcobacter spp. in the Coastal Environment of the Mediterranean Sea

Fera¹,

Maugeri

Gugliandolo

et al. 2004

Appl Environ Microbiol

109

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The occurrence of Arcobacter spp. was studied in seawater and plankton samples collected from the Straits of Messina, Italy, during an annual period of observation by using cultural and molecular techniques. A PCR assay with three pairs of primers targeting the 16S and 23S rRNA genes was used for detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii in cultures and environmental samples. Only one of the Arcobacter species, A. butzleri, was isolated from seawater and plankton samples. With some samples the A. butzleri PCR assay gave amplified products when cultures were negative. A. cryaerophilus and A. skirrowii were never detected by culture on selective agar plates; they were detected only by PCR performed directly with environmental samples. Collectively, our data suggest that culturable and nonculturable forms of Arcobacter are present in marine environments. The assay was useful for detecting Arcobacter spp. both as free forms and intimately associated with plankton. This is the first report showing both direct isolation of A. butzleri and the presence of nonculturable Arcobacter spp. in the coastal environment of the Mediterranean Sea.

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Helicobacter pylori in the Canadian arctic: seroprevalence and detection in community water samples

Cited by 36 publications

References 0 publications

Unexpected Decrease with Age of Helicobacter pylori Seroprevalence among Swedish Blood Donors

Unexpected Decrease with Age of Helicobacter pylori Seroprevalence among Swedish Blood Donors

Association Between HLA-DQ Genotypes and Haplotypes vs Helicobacter pylori Infection in an Indonesian Population

Detection of Arcobacter spp. in the Coastal Environment of the Mediterranean Sea

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