Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays

Silva, Lisete M.; Correia, Viviana G.; Moreira, Ana S. P.; Domingues, M. Rosário M.; Ferreira, Rui M.; Figueiredo, Céu; Azevedo, Nuno F.; Marcos-Pinto, Ricardo; Carneiro, Fátima; Magalhães, Ana C.; Reis, Celso A.; Feizi, Ten; Ferreira, José Alexandre; Coimbra, Manuel A.; Palma, Angelina S.

doi:10.1016/j.carbpol.2020.117350

Cited by 17 publications

(22 citation statements)

References 64 publications

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“…pylori induces inflammation, and Abs against H . pylori LPS were observed at high levels in HPI individuals ( 19 , 20 ). LPS from other bacterial species than H .…”

Section: Resultsmentioning

confidence: 99%

Metagenomic and single-cell RNA-Seq survey of the Helicobacter pylori–infected stomach in asymptomatic individuals

Sorini¹,

Tripathi²,

Wu³

et al. 2023

JCI Insight

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Helicobacter pylori colonization of the gastric niche can persist for years in asymptomatic individuals. To deeply characterize the host–microbiota environment in H . pylori –infected (HPI) stomachs, we collected human gastric tissues and performed metagenomic sequencing, single-cell RNA-Seq (scRNA-Seq), flow cytometry, and fluorescent microscopy. HPI asymptomatic individuals had dramatic changes in the composition of gastric microbiome and immune cells compared with noninfected individuals. Metagenomic analysis uncovered pathway alterations related to metabolism and immune response. scRNA-Seq and flow cytometry data revealed that, in contrast to murine stomachs, ILC2s are virtually absent in the human gastric mucosa, whereas ILC3s are the dominant population. Specifically, proportion of NKp44 + ILC3s out of total ILCs were highly increased in the gastric mucosa of asymptomatic HPI individuals, and correlated with the abundance of selected microbial taxa. In addition, CD11c + myeloid cells and activated CD4 + T cells and B cells were expanded in HPI individuals. B cells of HPI individuals acquired an activated phenotype and progressed into a highly proliferating germinal-center stage and plasmablast maturation, which correlated with the presence of tertiary lymphoid structures within the gastric lamina propria. Our study provides a comprehensive atlas of the gastric mucosa–associated microbiome and immune cell landscape when comparing asymptomatic HPI and uninfected individuals.

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“…pylori induces inflammation, and Abs against H . pylori LPS were observed at high levels in HPI individuals ( 19 , 20 ). LPS from other bacterial species than H .…”

Section: Resultsmentioning

confidence: 99%

Metagenomic and single-cell RNA-Seq survey of the Helicobacter pylori–infected stomach in asymptomatic individuals

Sorini¹,

Tripathi²,

Wu³

et al. 2023

JCI Insight

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“…Similar to mechanical microspotting, when using inkjet printing, several thousands of spots (with different concentrations of biomolecules or different biomolecules) can be printed on a single chip [ 76 , 77 , 78 , 79 ]. DNA [ 46 , 77 , 80 , 81 ], antibodies [ 82 , 83 ], glycans [ 84 ], proteins [ 40 , 78 , 79 , 85 ], and peptides [ 86 , 87 ] are fabricated using SciFLEXARRAYER, Nano-Plotter, and Arrayjet. Figure 1 B depicts a generic overview of the inkjet printing process.…”

Section: Microarray Fabrication Biomolecule Immobilization and Detect...mentioning

confidence: 99%

“…The use of fluorescent dyes (Cy3, Cy5, FITC, Alexa fluor 647, Alexa fluor 555, and Alexa fluor 488), radioisotopes ( 32 P, 33 P, and 14 C), near-infrared dyes (phthalocyanine dyes, polymethine carbocyanine dyes, IRDye 800CW, IRDye 750, and IRDye 800), and enzymes (horseradish peroxidase) falls under the category of label-dependent. Importantly, fluorescent dyes (Cy3 label, Cy5,FITC, Alexa flour 647, Alexa fluor 555, Alexa 488, CF555 or CF647, DyLight ® 650, DyLight ® 550 [ 69 ], and Green 540 [ 95 ]) were preferred in most of the microarray articles [ 46 , 49 , 54 , 56 , 57 , 58 , 61 , 65 , 71 , 72 , 73 , 77 , 82 , 83 , 84 , 93 , 97 , 134 , 135 , 136 ]. The reason for this could be their ease of availability, the established protocol to conjugate the dyes, their use for designing a multiplex assay, and the commercially available screening instrumentation (confocal microscope, LuxScan 10K, and GenePix pro 6).…”

Section: Microarray Fabrication Biomolecule Immobilization and Detect...mentioning

confidence: 99%

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Tetala

2023

Biomolecules

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Microarrays are one of the trailblazing technologies of the last two decades and have displayed their importance in all the associated fields of biology. They are widely explored to screen, identify, and gain insights on the characteristics traits of biomolecules (individually or in complex solutions). A wide variety of biomolecule-based microarrays (DNA microarrays, protein microarrays, glycan microarrays, antibody microarrays, peptide microarrays, and aptamer microarrays) are either commercially available or fabricated in-house by researchers to explore diverse substrates, surface coating, immobilization techniques, and detection strategies. The aim of this review is to explore the development of biomolecule-based microarray applications since 2018 onwards. Here, we have covered a different array of printing strategies, substrate surface modification, biomolecule immobilization strategies, detection techniques, and biomolecule-based microarray applications. The period of 2018–2022 focused on using biomolecule-based microarrays for the identification of biomarkers, detection of viruses, differentiation of multiple pathogens, etc. A few potential future applications of microarrays could be for personalized medicine, vaccine candidate screening, toxin screening, pathogen identification, and posttranslational modifications.

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“…The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/foods12020246/s1, Table S1: List of polysaccharides in the array; Table S2: List of proteins analyzed in the array. References [34][35][36][37][38][39] are cited in Supplementary Materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers

Reis

Martins

Bastos

et al. 2023

Foods

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Brewer’s spent yeast (BSY) microcapsules have a complex network of cell-wall polysaccharides that are induced by brewing when compared to the baker’s yeast (Saccharomyces cerevisiae) microcapsules. These are rich in (β1→3)-glucans and covalently linked to (α1→4)- and (β1→4)-glucans in addition to residual mannoproteins. S. cerevisiae is often used as a drug delivery system due to its immunostimulatory potential conferred by the presence of (β1→3)-glucans. Similarly, BSY microcapsules could also be used in the encapsulation of compounds or drug delivery systems with the advantage of resisting digestion conferred by (β1→4)-glucans and promoting a broader immunomodulatory response. This work aims to study the feasibility of BSY microcapsules that are the result of alkali and subcritical water extraction processes, as oral carriers for food and biomedical applications by (1) evaluating the resistance of BSY microcapsules to in vitro digestion (IVD), (2) their recognition by the human Dectin-1 immune receptor after IVD, and (3) the recognition of IVD-solubilized material by different mammalian immune receptors. IVD digested 44–63% of the material, depending on the extraction process. The non-digested material, despite some visible agglutination and deformation of the microcapsules, preserved their spherical shape and was enriched in (β1→3)-glucans. These microcapsules were all recognized by the human Dectin-1 immune receptor. The digested material was differentially recognized by a variety of lectins of the immune system related to (β1→3)-glucans, glycogen, and mannans. These results show the potential of BSY microcapsules to be used as oral carriers for food and biomedical applications.

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Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays

Cited by 17 publications

References 64 publications

Metagenomic and single-cell RNA-Seq survey of the Helicobacter pylori–infected stomach in asymptomatic individuals

Metagenomic and single-cell RNA-Seq survey of the Helicobacter pylori–infected stomach in asymptomatic individuals

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers

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