Helicobacter pylori resistance to antibiotics in Primorsko-goranska county

Bubonja‐Šonje, Marina; Gregorović-Kesovija, Palmira; Ambrožić, Damir; Pružinec-Popović, Blanka; Abram, Maja

doi:10.21860/medflum2018_203550

Cited by 1 publication

(1 citation statement)

References 6 publications

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“…Being a virulent opportunistic pathogen, it can cause serious infections in elderly and immunocompromised patients [10][11][12][13]. The major clinical concern nowadays is the overall presence of the carbapenem-resistant strain of K. pneumoniae, known as OXA-48-producing K. pneumoniae, which is slowly becoming more frequent-nosocomial strain in some European countries [14][15][16][17][18]. The main sources of contamination with K. pneumoniae in hospitals are in the gastrointestinal tract of both patients and staff, staff hands and the hospital environment indirectly and possibly in biofilm [10].…”

Section: Introductionmentioning

confidence: 99%

Disinfecting Action of Gaseous Ozone on OXA-48-Producing Klebsiella pneumoniae Biofilm In Vitro

Piletić

Kovač

Perčić

et al. 2022

IJERPH

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Klebsiella pneumoniae is an emerging multidrug-resistant pathogen that can contaminate hospital surfaces in the form of a biofilm which is hard to remove with standard disinfectants. Because of biofilm resistance to conservative disinfectants, the application of new disinfection technologies is becoming more frequent. Ozone gas has antimicrobial activity but there is lack of data on its action against K. pneumoniae biofilm. The aim of this study was to investigate the effects and mechanisms of action of gaseous ozone on the OXA-48-procuding K. pneumoniae biofilm. A 24 h biofilm of K. pneumoniae formed on ceramic tiles was subsequently exposed to different concentrations of ozone during one and two hours to determine the optimal ozone concentration. Afterwards, the total bacteria count, total biomass and oxidative stress levels were monitored. A total of 25 ppm of gaseous ozone was determined to be optimal ozone concentration and caused reduction in total bacteria number in all strains of K. pneumoniae for 2.0 log10 CFU/cm2, followed by reduction in total biomass up to 88.15%. Reactive oxygen species levels significantly increased after the ozone treatment at 182% for the representative K. pneumoniae NCTC 13442 strain. Ozone gas in the concentration of 25 ppm caused significant biofilm reduction but did not completely eradicate the K. pneumoniae biofilm formed on ceramics. In conclusion, ozone gas has great potential to be used as an additional hygiene measure in joint combat against biofilm in hospital environments.

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