Helicobacter pylori transiently in the mouth may participate in the transmission of infection

Abstract

Helicobacter pylori infection is associated with peptic ulcer and gastric carcinoma. The oral cavity may be a reservoir for H. pylori; however, the results of studies on this subject are controversial. We employed single-step and nested polymerase chain reactions (PCR) to detect the presence of the vacA, ureA and 16S rDNA genes of H. pylori in the stomach, saliva and dental plaque of 30 subjects. The results were confirmed by sequencing. Nested 16S rDNA and ureA amplification was achieved in 80% of gastric, 30% of saliva and 20% of dental plaque specimens. Sequencing of 10, seven and four 16S rDNA products from stomach, saliva and dental plaque, respectively, showed > 99% identity with H. pylori. Sequencing of the other four oral cavity PCR products showed similarity with Campylobacter and Wolinella species. Additionally, the vacA genotype identified in the samples of different sites was the same within a given subject.H. pylori may be found in the oral cavity of patients with gastric infection, thus it could be a source of transmission. However, results obtained with detection methods based only on PCR should be interpreted with caution because other microorganisms that are phylogenetically very close to H. pylori are also present in the mouth.

The Family Helicobacteraceae

“…The bacterium has also been isolated from sewage (Yoshinari 1980;Tanner et al 1984) and from humans (Radcliffe et al 1979). Wolinella DNA has been detected in the oral cavity of humans (Silva et al 2010), dogs (Petersen et al 2007;Craven et al 2011), and cats (Petersen et al 2007 …”

Comparación de Helicobacter pylori en cavidad oral y mucosa gástrica de acuerdo a genotipo de virulencia (cagA y vacAm 1)

“…Varios estudios han demostrado la presencia de H. pylori simultáneamente en cavidad oral y mucosa gástrica, mostrando diferentes prevalencias, dependiendo de la población estudiada, la selección de la muestra y técnicas de detección de la bacteria [26][27][28][29][30][31][32][33]40 . Mapstone 41 , demostró que la presencia de H. pylori en la boca de pacientes dispépticos es importante para su diseminación y considera la cavidad oral una potencial fuente de reinfección luego de la erradicación del microorganismo del estómago.…”

“…Algunos autores han encontrado una asociación entre la presencia de H. pylori en mucosa gástrica y oral [26][27][28][29][30][31][32][33] . En una investigación previa realizada por nuestro grupo, se observó la co-existencia de la bacteria en ambas localizaciones mediante RPC en tiempo real (RPC-TR) 34 .…”

Oral Cavity and Systemic Diseases—Helicobacter Pyloriand Dentistry

“…H. pylori was detected in the supragingival plaque and there was an association between the supragingival colonization of H. pylori and oral hygiene parameters such as the presence of plaque and gingival bleeding (23).…”

“…Silva et al (23) studied 115 individuals, divided in 4 groups: (A) with gastric diseases and periodontal disease; (B) with gastric diseases and no periodontal disease; (c) without gastric diseases and without periodontal disease, (D) without gastric diseases and with periodontal disease. H. pylori was detected in the supragingival plaque and there was an association between the supragingival colonization of H. pylori and oral hygiene parameters such as the presence of plaque and gingival bleeding (23).…”