Helicobacter pylori γ-Glutamyl Transpeptidase Is a Pathogenic Factor in the Development of Peptic Ulcer Disease

Gong, Ming; Ling, Samantha Shi Min; Lui, Sook Yin; Yeoh, Khay Guan; Ho, Bow

doi:10.1053/j.gastro.2010.03.050

Cited by 116 publications

(108 citation statements)

References 41 publications

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“…This enzyme has also been implicated in the virulence of Shigella flexneri and several S. enterica serovars such as Typhimurium, Gallinarum, and Dublin (55), suggesting that cytochrome bd may be particularly important for the growth and survival of pathogens that encounter environments in which O 2 is progressively limited. GGT (EC 2.3.2.2) is reported to contribute to the virulence of Helicobacter pylori in mice (56,57) and has been implicated in inhibition of T-cell proliferation and mediation of cell apoptosis (58,59). Another gene, yncD, encodes TonB-dependent transporters and was recently identified as an in vivo-induced antigen contributing to S. enterica serovar Typhi virulence in a murine model (60).…”

Section: Resultsmentioning

confidence: 99%

RNA Sequencing Reveals Differences between the Global Transcriptomes of Salmonella enterica Serovar Enteritidis Strains with High and Low Pathogenicities

Shah

2014

Appl Environ Microbiol

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Salmonella enterica serovar Enteritidis is one of the important causes of bacterial food-borne gastroenteritis worldwide. Field strains of S. Enteritidis are relatively genetically homogeneous; however, they show extensive phenotypic diversity and differences in virulence potential. RNA sequencing (RNA-Seq) was used to characterize differences in the global transcriptome between several genetically similar but phenotypically diverse poultry-associated field strains of S. Enteritidis grown in laboratory medium at avian body temperature (42°C). These S. Enteritidis strains were previously characterized as high-pathogenicity (HP; n ‫؍‬ 3) and low-pathogenicity (LP; n ‫؍‬ 3) strains based on both in vitro and in vivo virulence assays. Using the negative binomial distribution-based statistical tools edgeR and DESeq, 252 genes were identified as differentially expressed in LP strains compared with their expression in the HP strains (P < 0.05). A majority of genes (235, or 93.2%) showed significantly reduced expression, whereas a few genes (17, or 6.8%) showed increased expression in all LP strains compared with HP strains. LP strains showed a unique transcriptional profile that is characterized by significantly reduced expression of several transcriptional regulators and reduced expression of genes involved in virulence (e.g., Salmonella pathogenicity island 1 [SPI-1], SPI-5, and fimbrial and motility genes) and protection against osmotic, oxidative, and other stresses, such as iron-limiting conditions commonly encountered within the host. Several functionally uncharacterized genes also showed reduced expression. This study provides a first concise view of the global transcriptional differences between field strains of S. Enteritidis with various levels of pathogenicity, providing the basis for future functional characterization of several genes with potential roles in virulence or stress regulation of S. Enteritidis.

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Section: Resultsmentioning

confidence: 99%

RNA Sequencing Reveals Differences between the Global Transcriptomes of Salmonella enterica Serovar Enteritidis Strains with High and Low Pathogenicities

Shah

2014

Appl Environ Microbiol

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“…Three enzymes, a collagenase, secreted serine protease HtrA and g-glutamyl transpeptidase (Gong et al 2010;Rossi 2012, p. 523;Hoy et al 2010), also present in H. pylori and implicated in virulence, have also been detected in H. felis. Further, 52 outer membrane proteins belonging to the Hor, Hop, Hof, and Hom gene families have been detected.…”

Section: Other Helicobactersmentioning

confidence: 96%

The Family Helicobacteraceae

et al. 2014

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“…H. pylori 26695 WT and its cagA isogenic mutant strain (DcagA) expressing a chloramphenicol resistance gene cassette. H. pylori DcagA was constructed using a modified multistep polymerase chain reaction (PCR) approach (Gong et al, 2010). Bacteria were cultured on chocolate blood agar (Oxoid) supplemented with 5 % horse blood (Thermo Scientific).…”

Section: Methodsmentioning

confidence: 99%

Extracellular galectin-3 counteracts adhesion and exhibits chemoattraction in Helicobacter pylori-infected gastric cancer cells

Subhash

Ling

2016

Microbiology

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Helicobacter pylori γ-Glutamyl Transpeptidase Is a Pathogenic Factor in the Development of Peptic Ulcer Disease

Cited by 116 publications

References 41 publications

RNA Sequencing Reveals Differences between the Global Transcriptomes of Salmonella enterica Serovar Enteritidis Strains with High and Low Pathogenicities

RNA Sequencing Reveals Differences between the Global Transcriptomes of Salmonella enterica Serovar Enteritidis Strains with High and Low Pathogenicities

The Family Helicobacteraceae

Extracellular galectin-3 counteracts adhesion and exhibits chemoattraction in Helicobacter pylori-infected gastric cancer cells

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