Helminthes detected from wild sika deer (Cervus nippon centralis) in Kanto-Chubu region, Japan

Inoue, Ken; Shishida, Kohei; Kawarai, Shinpei; Takeda, Shiro; Minami, Masato; Taira, Kensuke

doi:10.1016/j.parint.2021.102485

Cited by 2 publications

(1 citation statement)

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“…from Vietnam [6], Brazil [18], and Sri Lanka [4] were used as reference sequences for tree construction. In addition, a sequence of Dipylidium caninum from Italy (MT806359) [25] and Dicrocoelium dendriticum from Japan (LC629058) [26] were used as an outgroup species for the cox1 gene and ITS2 region, respectively. Multiple sequences were aligned using ClustalW, and phylogenetic analysis was performed using a maximum likelihood (ML) method based on the Tamura-Nei model [27].…”

Section: Molecular Technique For Species Identificationmentioning

confidence: 99%

Occurrence and Risk Factors Associated with Platynosomum illiciens Infection in Cats with Elevated Liver Enzymes

Chantawong,

Potiwong,

Choochote

et al. 2024

Animals

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Platynosomum spp., a hepatic trematode, causes fatal hepatobiliary disease in cats. Feline platynosomiasis is often underestimated due to a lack of awareness and diagnostic challenges. This study aimed to investigate the occurrence, factors, and clinicopathological abnormalities associated with Platynosomum spp. infection in cats with elevated serum ALT levels. Platynosomum infection was determined using zinc sulfate flotation and formalin–ether sedimentation. DNA sequence analysis of PCR products from the Platynosomum internal transcribed spacer 2 (ITS2) region and cox1 gene was used to identify Platynosomum species. Of a total of 43 cat fecal samples, the proportion of Platynosomum spp. infection by microscopic examination was 11.63% (5/43). All PCR-positive samples were molecularly identified as Platynosomum illiciens. From the logistic regression analysis, the odds of Platynosomum infection in cats without a deworming program were 16 times higher than those of regularly dewormed cats. Demographic data, housing conditions, and predatory behavior were not significantly associated with the infection. Regarding blood profiles, infected cats had higher eosinophil counts (p = 0.014), with no significant differences in ALT (p = 0.791) or ALP (p = 0.970) levels compared to non-infected cats. Our findings demonstrate that eosinophilia in cats with increased serum ALT may suggest P. illiciens infection in endemic areas. We strongly recommend a regular deworming program to mitigate the risk of P. illiciens infection.

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