Helplessness in the Tail Suspension Test Is Associated with an Increase in Ethanol Intake and Its Rewarding Effect in Female Mice

Pelloux, Yann; Hagues, Guillaume; Costentin, Jean; Duterte-Boucher, Dominique

doi:10.1097/01.alc.0000156123.10298.fa

Cited by 18 publications

(17 citation statements)

References 60 publications

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“…However, these studies are in direct contrast to research indicating that an acute or chronic stressor increases ethanol consumption in mice (i.e. Chester et al, 2006; Farook et al, 2009; Lopez et al, 2011; Matthews et al, 2008; Pelloux et al, 2005; Racz et al, 2003; reviewed in Becker et al, 2011). With variations in stressors administered, genotype and sex of the mouse used, and ethanol access paradigm employed, interpretation of the impact of stress on ethanol consumption in mice is difficult.…”

Section: Introductionmentioning

confidence: 80%

“…Traditionally, tail suspension stress (more commonly referred to as tail suspension test or TST) is used to assess depression-like symptoms (see Cryan et al, 2005 for details), and one study investigated the relationship between immobility time in the TST and ethanol consumption in male and female CD-1 mice (Pelloux et al, 2005). Placing mice into subgroups for high immobility (HI) versus low immobility (LI) time did not influence ethanol intake in male mice.…”

Section: Discussionmentioning

confidence: 99%

“…Behavior was measured as latency to first period of immobility (a 2 second period during which no movement occurred), time spent immobile, percent of time spent immobile, and total episodes of immobility. Behavior was scored by two technicians and then averaged (see Table 1; Barfield et al, 2010; Pelloux et al, 2005). …”

Section: Methodsmentioning

confidence: 99%

“…We used a drinking procedure in which animals consume ethanol at levels that result in physiologically relevant blood ethanol concentrations (BECs; e.g., Finn et al, 2004) to test the hypothesis that the subsequent ethanol consumption following stress exposure would vary in a sex- and stressor-dependent way. In support of this general hypothesis, limited available data in other genotypes indicate that repeated restraint stress significantly increased ethanol intake in male and significantly decreased ethanol intake in female mice that were selectively bred for high alcohol preference (HAP; Chester et al, 2006), and that tail suspension stress significantly increased ethanol intake only in female but not in male CD-1 mice with high immobility scores (Pelloux et al, 2005). Finally, because perceived predation risk has a profound biological effect across species (Zanette et al, 2011), and predator odor stress significantly increased ethanol self-administration in male Wistar rats (Edwards et al, 2013; Roltsch et al, 2014), we predicted that exposure to predator odor stress would significantly increase ethanol intake and that the effect might be more pronounced in female mice.…”

Section: Introductionmentioning

confidence: 91%

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Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner

Cozzoli

Tanchuck-Nipper

Kaufman

et al. 2014

Alcohol

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Environmental factors, including exposure to stress, are known to contribute to the propensity to consume ethanol. However, stress produces inconsistent effects on ethanol drinking in rodent models. Therefore, the present study examined the impact of different stressors on limited access ethanol consumption and determined whether there were sex-dependent differences in response to stress. To this end, male and female C57BL/6J mice had 2-hr access to a water and 10% ethanol solution, beginning 30 minutes before onset of the dark cycle. Once ethanol intake was stable, the effect of restraint, tail suspension, predator odor, foot shock, and tail pinch on subsequent intake was explored. Both plasma corticosterone (CORT) and allopregnanolone (ALLO) were assessed as indices of hypothalamic-pituitary-adrenal (HPA) axis activity and of endogenous neurosteroid levels respectively, following restraint, tail suspension and predator odor. Ethanol intake was decreased following restraint, tail suspension, foot shock, and tail pinch in both sexes, with stressor-related differences in the duration of the suppression. The effect of predator odor on ethanol intake was biphasic in females; ethanol consumption was significantly reduced on the day of stress but significantly increased on the following two days. In males, predator odor produced a delayed significant increase in ethanol intake on the second day after stress. All three stressors increased plasma CORT, with higher CORT levels in females when compared with males. Notably, there was a significant positive correlation between CORT levels immediately after predator odor stress and ethanol intake on the following day as well as a significant positive linear relationship between CORT levels immediately after restraint stress and ethanol intake on the following day in females. Furthermore, the three stressors produced a greater increase in ALLO levels in female versus male mice, but ALLO levels following predator odor were not correlated with subsequent ethanol intake. In summary, the type of stressor administered had a profound impact on subsequent ethanol consumption, with subtle sex differences in the magnitude and persistence of the effect. These findings are the first to demonstrate that a single, acute exposure to restraint, tail suspension, and predator odor stress increased plasma CORT and ALLO levels in animals with a history of ethanol consumption and that female mice were more responsive than males to the ability of stress to increase CORT levels as well as to the ability of predator odor stress to produce a delayed increase in ethanol intake. Because predator odor stress is a model of posttraumatic stress disorder, the present sex differences have important implications for future preclinical studies modeling the comorbidity of posttraumatic stress disorder and alcohol use disorders.

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Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

See 2 more Smart Citations

Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner

Cozzoli

Tanchuck-Nipper

Kaufman

et al. 2014

Alcohol

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show abstract

“…The behavioral tests were selected for their predictive sex differences where females typically show more passive responses than males (15,16,54,55). In humans, passive coping is more prevalent in females and is associated with higher depressive scores (17,18).…”

Section: Discussionmentioning

confidence: 99%

Organizational and Activational Effects of Testosterone on Masculinization of Female Physiological and Behavioral Stress Responses

Goel

Bale

2008

Endocrinology

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The prevalence of affective disorders is two times greater in women than in men. The onset of anxiety and depression occurs at different ages that may correspond to key developmental periods when the brain is more vulnerable to hormonal and exogenous influences. Because stressful life events can precipitate disease onset, the development of greater stress sensitivity in females may contribute to their increased vulnerability. Gonadal hormone exposure in males during early development and again from puberty onward plays a prominent role in sexually dimorphic brain formation, possibly contributing to sex differences in stress responsivity. Therefore, organizational effects of testosterone propionate (TP) administered postnatally and activational effects of TP administered beginning at puberty on adult female physiological and behavioral stress responses were examined in mice. Although the activational effects of TP in females ameliorated the sex difference in the hypothalamic-pituitary-adrenal axis stress response, there was no effect of postnatal TP. Similarly, higher immobile time in intact females in the tail suspension test was blunted by activational TP in the absence of postnatal TP. However, in the marble-burying test of anxiety-like behaviors, organizational and activational TP independently resulted in increased burying behaviors. These results show that TP administration has distinct effects on reducing physiological and behavioral stress responsivity in rodent models and suggest that sex differences in these responses may partially result from the absence of testosterone in females.

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The Tail-Suspension Test: A Model for Characterizing Antidepressant Activity in Mice

O’Leary

Cryan

2009

Mood and Anxiety Related Phenotypes in Mice

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Helplessness in the Tail Suspension Test Is Associated with an Increase in Ethanol Intake and Its Rewarding Effect in Female Mice

Cited by 18 publications

References 60 publications

Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner

Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner

Organizational and Activational Effects of Testosterone on Masculinization of Female Physiological and Behavioral Stress Responses

The Tail-Suspension Test: A Model for Characterizing Antidepressant Activity in Mice

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