Hemagglutinating activity associated with bovine herpesvirus type 1

Trépanier, Pierre; Séguin, Cécile; Bastien, Yolande; Boulay, Gaston; Lussier, G; Trudel, Michel

doi:10.1016/0378-1135(85)90060-4

Cited by 23 publications

(12 citation statements)

References 2 publications

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“…Recently, hemagglutination activity associated with BHV-1 has been reported [40]. In the present study, specific experiments were carried out in order to identify the hemagglutinating protein as well as the VAP of BHV-1 using monoclonal antibodies directed against the glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

Bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination

Okazaki

Honda

Minetoma

et al. 1987

Archives of Virology

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The 87,000-dalton glycoprotein (gp87) of bovine herpesvirus type 1 (BHV-1) was found to selectively attach to susceptible cells. The attachment of gp87 to the cells was markedly decreased by the prior adsorption of intact virions. Anti-gp87 (site Ia) monoclonal antibody, which inhibited BHV-1 adsorption to the cells and neutralized the virus without complement [Okazaki et al., Virology 250: 260-264], was effective in inhibiting the adsorption of gp87. Only the same antibody was able to inhibit the hemagglutination activity of BHV-1. Other monoclonal antibodies to the glycoproteins of BHV-1, including antibodies directed to sites Ib and Ic on gp87, were ineffective in inhibiting either virus adsorption or hemagglutination. The results of this study indicate that site Ia of gp87 molecule is the critical site of virus attachment for initiation of infection as well as the hemagglutination of BHV-1.

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Section: Introductionmentioning

confidence: 99%

Bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination

Okazaki

Honda

Minetoma

et al. 1987

Archives of Virology

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“…Red blood cells from various mammalian and avian sources were collected and washed in Alsever buffer [15] and resuspended in the appropriate buffers. Ten different buffers were tested: (i) TNE (10 mM Tris-HCl, pH phosphate-buffered saline with 1% kaolintreated FCS [18], and (7-10) BABS, pH 5.75, 6.0, 6.2, or 6.4, respectively [16], The hemag glutination assay was performed as de scribed previously [18], except that various buffers were used, red blood cells were made up to 0.3 or 0.5% (v/v) in the appropriate buffer, and microtiter plates were incubated at 4, 22, or 37°. Control hemagglutinating antigens were either rabbit enteric coronavirus (titer 1/64 with rabbit red blood cells ) or pneumonia virus of mice (titer 1/320 with CDI mouse erythrocytes).…”

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confidence: 99%

Hemagglutination by Murine Hepatitis Viruses

Talbot¹

1989

Intervirology

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Erythrocytes from twelve mammalian and avian sources in ten different buffers at three incubation temperatures could not be hemagglutinated with murine hepatitis virus (MHV) strains 3, A59, or S grown on DBT cells. Viral antigen preparation in the absence of fetal calf serum, partial virus purification, or various concentrations of red blood cells still failed to yield detectable hemagglutinating activity. Thus, the newly described MHV-DVIM remains the only hemagglutinating strain of murine coronavirus.

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“…Glycoproteins, being located on the viral envelope and on the surface of virus-infected cells [8,17,28], play important roles during viral infection such as recognizing receptor sites for attachment and penetration of virus in cells. Hemagglutinating activity of several BHV-I strains has been reported [23] and this function assigned to gIII glycoprotein [24].…”

Section: Introductionmentioning

confidence: 98%

Gene mapping of infectious bovine rhinotracheitis viral DNA genome

Simard

Nadon

Séguin³

et al. 1990

Archives of Virology

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A bovine herpesvirus I (BHV-I) HindIII genomic bank spanning 89% of the entire genome was constructed and individual fragments analyzed for their capacity to select specific mRNAs which were then expressed by in vitro translation assays. This procedure allowed the mapping of more than 20 viral polypeptides to discrete regions of the DNA genome. Some polypeptides map in neighboring HindIII fragments while most seem encoded in single fragments. In particular, the coding sequences for an abundant 94 kDa polypeptide, which is the potential unglycosylated precursor of gII glycoprotein, have been assigned to the small 3.6 kbp HindIII genomic fragment M. The localization of structural and non-structural gene-coding sequences will help to characterize viral polypeptides and eventually, a better understanding of BHV-I infection will be gained.

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Hemagglutinating activity associated with bovine herpesvirus type 1

Cited by 23 publications

References 2 publications

Bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination

Bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination

Hemagglutination by Murine Hepatitis Viruses

Gene mapping of infectious bovine rhinotracheitis viral DNA genome

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