Hemagglutination is a novel biological function of lipopolysaccharide (LPS), as seen with the Vibrio cholerae O139 LPS

Abstract

It has been generally thought that the polysaccharide moiety of lipopolysaccharide (LPS) maintains only serological specificity, while the lipid A portion determines various biological functions. However, we found that hemagglutination was a common function of the polysaccharide moiety of LPSs from important human enteropathogenic bacteria. Of the LPSs examined, Vibrio cholerae O139 LPS showed the highest hemagglutinating activity. Glycoproteins, such as mucin and fetuin, showed efficient inhibition of the hemagglutinating ability. Since cell-mediated hemagglutination is known to be correlated with bacterial adherence, hemagglutination induced by the polysaccharide moiety is interpreted to indicate that cell-surface LPS is a potential adhesin.

Effect of parenteral endotoxin administration on the immuno-haematological responses of catfish, Heteropneustes fossilis

“…Likewise, the agglutination titre in irrespective of endotoxin injected groups indicated a possible enhancement of natural agglutinin level in serum of the treated fish due to leucocyte proliferation and differentiation. Furthermore, hemagglutination activity is believed to be an important biological function of LPS [50] and this is also apparent from the findings of the present study i.e., irrespective of doses agglutinating activity of H. fossilis serum increased in responses to endotoxin.…”

The anti‐endotoxic effects of the KSL‐W decapeptide on Escherichia coli O55:B5 and various oral lipopolysaccharides

“…The ability of lipopolysaccharide to induce erythrocyte hemagglutination has been reported as a functional property of the direct interaction of erythrocytes with differing segments within the polysaccharide portion (30,33,42) as well as with the lipid A segment (43) of the lipopolysaccharide molecule. Therefore, to explore whether KSL‐W binding to lipopolysaccharide would alter the lipopolysaccharide‐induced hemagglutination of rabbit erythrocytes, we compared various E. coli lipopolysaccharide sample concentrations pre‐incubated with the KSL‐W peptide with matched lipopolysaccharide control samples alone (Fig.…”

“…It has been well reported that lipopolysaccharide from various bacteria can induce hemagglutination in erythrocytes (30–33). We therefore tested the ability of the KSL‐W peptide to block E. coli O55:B5 lipopolysaccharide‐induced hemagglutination.…”

Survival of Vibrio cholerae Inside Acanthamoeba and Detection of Both Microorganisms From Natural Water Samples May Point out the Amoeba as a Protozoal Host for V. cholerae

“…It was shown that growth of wild type V. cholerae O139 MO10, capsule mutant strain, and capsule + lipopolysaccharide (LPS) double mutant strain were enhanced in the presence of A. castellanii [10]. Previous studies have shown that both capsule and LPS O side chain of V. cholerae MO10 enhance adherence to the human intestinal mucosa [68,69] and the capsule may contribute to partially resist phagocytosis [70].…”