Hemagglutination with Adenoviruses. Nature of Viral Antigen

Thirty two mouse ascitic fluids containing monoclonal antibodies (MAbs) directed against the hexon of human adenovirus type 1 were examined. The type and degree of cross-reactivity (CR) of the MAbs were determined by ELISA and hemagglutination methods with 10 heterologous hexon types. The similarity and the dissimilarity of the MAbs was also characterized by the correlation coefficient calculated from their reactivity values. On the basis of these, the 32 MAbs could be divided into five groups and fourteen individual MAbs, which altogether recognized 19 distinct epitopes. One of the recognized epitopes is the genus specific epitope of adenovirus hexons; the others are interspecies specific ones which can be found on the surface of the different hexon types in characteristic, mosaic-like combinations. The type and degree of CR of the MAbs lead to the conclusion that there exists a close antigenic relationship among the members of subgenus C of human adenoviruses and there is also a definite antigenic relationship between subgenera C and D. Hexons belonging to the oncogenic subgenera A and B display a much looser antigenic relationship with subgenus C.

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“…Passive Hemaggluti~ation (HA) HA was carried out according to the method by Ross and GINSBE~C (11) and modified by us (1).…”

Section: Enzymelinked Immunosorbent Assay (Elisa )mentioning

confidence: 99%

Grouping of monoclonal antibodies to adenovirus hexons by their cross-reactivity

Ádám

Lengyel

Takács

et al. 1986

Archives of Virology

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“…The maintenance and infection of the cells and the separation and purification of hexon pro teins were done according to methods de scribed earlier [11,12], Supernatant fluids of the 60 hybridoma lines were tested for their cross-reactivity with hexon preparations of nine different heterologous human adenovirus species (ser ological types) belonging to four different subgenera [4] by enzyme-linked immunosor bent assay (ELISA) [13] and by passive hem agglutination (HA). For the HA test, sheep red blood cells were sensitized with purified hexons [14]. Figure 1 shows the frequency distribution of monoclonal antibodies reacting with the different hexon types and the differences be tween the two assay systems.…”

mentioning

confidence: 99%

Delineation of Antigenic Determinants of Adenovirus Hexons by Means of Monoclonal Antibodies

Ádám¹,

Erdei

Lengyel

et al. 1985

Intervirology

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60 hexon-specific mouse hybridoma clones were selected by using crystallized hexon capsomers of human adenovirus type 1 as immunizing and selecting antigen. The reactivities of the monoclonal antibodies were tested with purified hexon preparations from 10 human adenovirus types by enzyme-linked immunosorbent assay and passive hemagglutination techniques. The reactivity patterns delineated a total of 41 adenovirus-type-1-related determinants present on a number of heterologous hexons in different interspecies combinations. The pattern of interspecies specificities did not coincide with the current grouping of adenoviruses into subgenera.

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A radiometric method for determination of the oil drop size distribution in hydrocarbon–water systems

Borzani

Podlech

1971

Biotech & Bioengineering

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Hemagglutination with Adenoviruses. Nature of Viral Antigen

Cited by 10 publications

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Grouping of monoclonal antibodies to adenovirus hexons by their cross-reactivity

Grouping of monoclonal antibodies to adenovirus hexons by their cross-reactivity

Delineation of Antigenic Determinants of Adenovirus Hexons by Means of Monoclonal Antibodies

A radiometric method for determination of the oil drop size distribution in hydrocarbon–water systems

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