Hematobiochemical alterations and direct blood polymerase chain reaction detection of Theileria annulata in naturally infected crossbred cows

Ganguly, Anita; Bhanot, Vandna; Bisla, R. S.; Ganguly, Indrajit; Singh, Harpreet; Chaudhri, S. S.

doi:10.14202/vetworld.2015.24-28

Cited by 17 publications

(11 citation statements)

References 12 publications

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“…In the present study, increased total leukocyte counts (9.31±0.37) was recorded as compared to uninfected control (8.37±0.24). The finding is in consonance with the findings of Modi et al, 2015;Ganguly et al, 2015 andGoyal et al, 2017. However, after increase in TLC, significant decrease within several days in Theileria affected cattle was recorded by Sandhu et al, (1998 Tehrani et al, 2013 andGoyal et al, 2017. In contrast to present study, Aulakh et al, (1998) observed lymphocytosis and neutropenia in infected cattle, while Omar et al, (2002) found lymphocytopenia and neutropenia.…”

Section: Resultssupporting

confidence: 91%

Estimation of Haematological Changes in Crossbred Cattle Infected with Theileria annulata

Kumar¹,

Vatsya²

2021

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 91%

Estimation of Haematological Changes in Crossbred Cattle Infected with Theileria annulata

Kumar¹,

Vatsya²

2021

Int.J.Curr.Microbiol.App.Sci

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“…Moreover, they were within normal range (Jain, 1993) indicating normocytic hypochromic anaemia (Table 1).However, the type of anaemia largely depends on the disease severity and accordingly various types of anaemia in babesiosis and theileriosis has been reported (Durrani et. al., 2006;Sharma et al, 2013, Mbassa et al, 1994Omer et al, 2002;Raminet al, 2011;Ganguly et al, 2015). In the present study, normocytic hypochromic anaemia observed in the B. bigamina infected crossbred cattle may be attributed to intravascular haemolysis of red blood cells (Pandy and Misra, 1987).…”

Section: Resultsmentioning

confidence: 57%

Direct blood PCR detection of Babesia bigemina and its effect on haematological and biochemical profile in crossbred cattle of eastern Haryana

Ganguly

Bisla

Ganguly

et al. 2017

IJAR

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The present study aimed to diagnose Babesia bigemina in naturally infected crossbred cows and to determine its effect on haemato-biochemical profile of host animals. Blood samples from lactating crossbred cows (n=30) between 3-6 years of age and showing clinical signs of babesiosis were collected, with or without anticoagulant, and analyzed for the protozoa by direct smear, direct blood PCR detection of the apical membrane antigen 1 (AMA-1) gene specific amplicon of B. bigemina and estimation of haematological and biochemical parameters. Healthy crossbred cows (n=10), examined free from haemoprotozoan infections were included as control. Blood Direct PCR revealed a 448-bp amplified fragment. Out of 150 random blood samples screened, (27/150) 18% were positive under light microscope, whereas direct blood PCR revealed (39/150) 26% samples positive for B. bigemina. The result shows higher specificity and sensitivity of PCR test over blood smear examination. The infected group showed significantly (p<0.001) decreased levels of TEC (3.04±0.19), Hb (4.78±0.27) and PCV (14.53 ±0.87) than healthy control animals. However, differences in the red blood cell indices (MCV, MCH and MCHC) were non-significant (p>0.05) between the groups indicating normocytic hypochromic anaemia in affected crossbred cattle. Serum samples of infected cows showed significantly (p<0.01) higher values of ALT (78.83±8.95), AST (146.13±7.62), BUN (27.09±1.02), creatinine (1.93±0.1) and TBIL (1.42±0.06) than that of healthy control. A significant decrease (p<0.01) of TSP (6.12±0.13) and albumin (2.39±0.09) was also recorded in the infected cows compare to healthy control. The standardized blood direct PCR method of the present investigation may be useful for rapid and reliable diagnosis of B. bigemina in conjunction with microscopic examination. Moreover, marked changes in haematological and serum biochemical profile observed in B. bigemina infected crossbred cows may be useful in understanding disease pathogenesis and undertaking necessary corrective measures.

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“…The increase in lymphocyte percentage might be due to persistent harmful effect of toxic metabolites of Theileria on hemopoietic organs specially bone marrow and there interference with the process of leucogenesis and proliferation of lymphocytes in the lymphoid organ as defensive response to invading parasite. The lymphocytosis might be due to intralymphocytic Theileria parasites transforming the host cell, leading to clonal growth of lymphocytes (Ganguly et al, 2015). The change in monocyte percentage might be attributed to persistent harmful effect of Theileria spp.…”

Section: Resultsmentioning

confidence: 99%

Efficacy of Arteether Against Theileriosis in Cattle

Khawale¹

2019

JAR

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The present study was done with the objective of comparative efficacy of arteether and buparvaquone against theileriosis in cattle. Total 67 cattle suspected for theileriosis were screened on the basis of clinical and blood smear examination. Group I (n=6) was treated with Inj. buparvaquone @ 2.5 mg/kg body weight once and Group II (n=6) was treated with Inj. arteether @5 mg/ kg body weight intramuscularly for three consecutive days. Rectal temperature, heart rate and respiration rate were significantly increased before treatment and in both the groups after treatment showed significant improvement. Haematological parameters showed significant decreased in Hb, PCV, TEC, TLC and neutrophil and significant increase in lymphocytes, monocytes and eosinophil. Non significant difference in basophil count was observed. After treatment, significant improvement was observed in mean hemoglobin, PCV, TEC, TLC, neutrophil, lymphocyte and eosinophil. Non significant improvement was observed in monocyte and basophil count. Present study revealed percent efficacy of arteether was 66.66% and buparvaquone 100%.

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Hematobiochemical alterations and direct blood polymerase chain reaction detection of Theileria annulata in naturally infected crossbred cows

Cited by 17 publications

References 12 publications

Estimation of Haematological Changes in Crossbred Cattle Infected with Theileria annulata

Estimation of Haematological Changes in Crossbred Cattle Infected with Theileria annulata

Direct blood PCR detection of Babesia bigemina and its effect on haematological and biochemical profile in crossbred cattle of eastern Haryana

Efficacy of Arteether Against Theileriosis in Cattle

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