Hematocrit-Independent Recovery is A Key for Bioanalysis Using Volumetric Absorptive Microsampling Devices, Mitra ^Tm

Abstract

Optimization of extraction process to achieve high recovery regardless of Hct is critical in accurate bioanalysis via Mitra.

Assessing Volumetric Absorptive Microsampling Coupled with Stable Isotope Dilution Assay and Liquid Chromatography–Tandem Mass Spectrometry as Potential Diagnostic Tool for Whole Blood 5-Methyltetrahydrofolic Acid

“…This was attributed to the higher relative amount of erythrocytes inside the tip determining the degree of analyte desorption (34). To overcome these effects, sonication (35) was shown to improve analyte recovery at high hematocrit values. The major part of WB folate is trapped in erythrocytes.…”

Development and validation of volumetric absorptive microsampling coupled with UHPLC–MS/MS for the analysis of gamma‐hydroxybutyric acid in human blood

“…Results from available literature show that analytical data by VAMS are mostly unaffected by even extreme HCT values (as low as 20%, as high as 70%) (Protti et al, 2019). It has been noticed that high extraction recovery is critical to lessen or eliminate potential HCT effects (Mano et al, 2015;Protti et al, 2019).…”

“…In particular, dried blood spot (DBS) has been preliminarily reported as a reliable alternative for classic venous sampling for GHB determination (Forni, Pearl, Gibson, Yu, & Sweetman, 2013;Ingels et al, 2013;Sadones et al, 2015;Saracino et al, 2015). Volumetric absorptive microsampling (VAMS) is a relatively recent microsampling technique used to obtain dried specimens of blood and other biological matrices for quantitative bioanalyses, and is claimed to bring some significant advantages over DBS, in particular better sampling volume accuracy and hematocrit (HCT) independence (Mano, Kita, & Kusano, 2015;Protti, Mandrioli, & Mercolini, 2019;Spooner et al, 2015).…”

Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

“…We adopted a volumetric absorptive microsampling (VAMS) device, which allows the simple collection of an accurate volume of blood [ 17 ]. Compared to the classical non-volumetric-based dried blood spots (DBS) analysis, the performances of this device are not influenced by the hematocrit (HCT) values and by the erythrocyte volume fraction [ 17 , 18 , 19 , 20 , 21 ]. VAMS approach has been yet adopted for several quantitative methods for several molecules in different fields [ 19 , 22 , 23 ].…”