Hematologic Phoulems Is the Newborn

Oski, F. A.; Naiman, J. Lawrence

doi:10.1097/00000441-196702000-00020

Cited by 99 publications

(136 citation statements)

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“…(3,6) Reference hematological values for cord blood have not been standardized, and routinely out of date values proposed in the literature are used. (7) In published studies, information on hematological values in cord blood is limited and inconsistent. The studies that we found (3)(4)(5)(6)(8)(9)(10) evaluated different subgroups of neonates and different hematological parameters.…”

Section: Introductionmentioning

confidence: 99%

Erythrocyte indices and serum ferritin in newborns

Nunes

Assis²,

Pinheiro³

et al. 2010

Rev. Bras. Hematol. Hemoter.

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Section: Introductionmentioning

confidence: 99%

Erythrocyte indices and serum ferritin in newborns

Nunes

Assis²,

Pinheiro³

et al. 2010

Rev. Bras. Hematol. Hemoter.

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“…This method, therefore, has realistic potential clinical application for the determination of fetal sex from the rare cells isolated from maternal blood during pregnancy. If the fetal DNA obtained from the cells sorted in this manner can be enzymatically amplified (16), then this approach might be extended to provide prenatal diagnosis for a broader range of genetic disorders. DIRECT who lacks this sequence might appear as a female.…”

Section: Discussionmentioning

confidence: 99%

“…The respective cloned inserts were isolated by electrophoresis and labeled with 32P dCTP by a n oligonucleotide primer method (6). After incubation with labeled probe at 68°C for 16 hours, the filters were removed and washed for 20 minutes in 0.1 x SSC and 0.1% SDS a t room temperature. They were then washed in the same bufier at 65°C for 1 hour, dried, and applied to Kodak X-omat AR film.…”

Section: Dna Hybridization Studiesmentioning

confidence: 99%

Direct hybridization to DNA from small numbers of flow‐sorted nucleated newborn cells

et al. 1987

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A technique is described that allows direct hybridization to the DNA of cells flow sorted onto nitrocellulose filters, which obviates an intervening DNA isolation step. The feasibility of this technique for studying small numbers of cells is demonstrated with human cord blood, which has a high proportion of nucleated cells. The cells are stained with fluorescein‐conjugated anti‐HLe‐l, a monoclonal antibody that recognizes mature leucocytes. Anti‐HLe‐l‐positive cells are all nucleated, and a controlled, precise number of them may be sorted directly onto a nitrocellulose membrane. In cord blood, a small percentage of anti‐HLe‐l‐negative cells are nucleated erythrocytes, which may also serve as a source of DNA. Studies were performed on male or female newborn cells flow sorted onto introcellulose membranes and hybridized with either a nonspecific human repeat DNA probe or a Y chromosome‐specific probe. Importantly, the sex of the newborn could be determined at the DNA level from as few as 50 sorted cord blood leucocytes or 5,000 HLe‐l‐negative cells. Since nucleated erythrocytes are common in fetal blood but rarely found in the peripheral circulation of adults, the method has potential application for the determination of fetal sex from analysis of flow‐sorted nucleated erythrocytes present in the maternal circulation during pregnancy.

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“…24 The degrees of anemia are various in different cases. Serum vitamin B12 levels were found to be normal in the reported cases1 and in our own case, and megaloblastic changes have been uniformly absent in these cases.…”

Section: Discussionmentioning

confidence: 99%