Hematological and biochemical reference values for C57BL/6, Swiss Webster and BALB/c mice

Santos, Ed Wilson; Oliveira, Dalila Cunha de; Hastreiter, Araceli Aparecida; Silva, Graziela Batista da; Beltran, Jackeline Soares de Oliveira; Tsujita, Maristela; Crisma, Amanda R.; Neves, Silvânia Meiry Peris; Fock, Ricardo Ambrósio; Borelli, Primavera

doi:10.11606/issn.1678-4456.v53i2p138-145

Cited by 106 publications

(92 citation statements)

References 5 publications

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“…We observed no significant change in these hematological parameters. Further, our results fell within the permittable range based on references from nonpregnant 2–3 months old male C57BL/6j mice for hematological studies (Santos et al, ).…”

Section: Discussionsupporting

confidence: 82%

“…Legend applies to all graphs. Reference range for nonpregnant C57BL/6j shown in grey (Santos et al, )…”

Section: Resultsmentioning

confidence: 99%

“…Legend applies to all graphs. Reference range for nonpregnant C57BL/6j shown in grey (Santos et al, 2016) F I G U R E 7 Percent body weight of major maternal organs upon necropsy on gestational day 17. Percent body weight on GD 17 of uteri, liver, kidneys, spleen of treated and control mice assessing toxicity-related weight changes in major organs involved in clearance of iron oxide nanoparticles.…”

Section: Assessment Of Mar Ldh B Autoantibody Capture In Vitromentioning

confidence: 99%

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Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Bolandparvaz

Vapniarsky

Harriman

et al. 2020

J Biomedical Materials Res

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In pursuit of a preventive therapeutic for maternal autoantibody‐related (MAR) autism, we assessed the toxicity, biodistribution, and clearance of a MAR specific peptide‐functionalized dextran iron oxide nanoparticle system in pregnant murine dams. We previously synthesized ~15 nm citrate‐coated dextran iron oxide nanoparticles (DIONPs), surface‐modified with polyethylene glycol and MAR peptides to produce systems for nanoparticle‐based autoantibody reception and entrapments (SNAREs). First, we investigated their immunogenicity and MAR lactate dehydrogenase B antibody uptake in murine serum in vitro. To assess biodistribution and toxicity, as well as systemic effects, we performed in vivo clinical and post mortem pathological evaluations. We observed minimal production of inflammatory cytokines—interleukin 10 (IL‐10) and IL‐12 following in vitro exposure of macrophages to SNAREs. We established the maximum tolerated dose of SNAREs to be 150 mg/kg at which deposition of iron was evident in the liver and lungs by histology and magnetic resonance imaging but no concurrent evidence of liver toxicity or lung infarction was detected. Further, SNAREs exhibited slower clearance from the maternal blood in pregnant dams compared to DIONPs based on serum total iron concentration. These findings demonstrated that the SNAREs have a prolonged presence in the blood and are safe for use in pregnant mice as evidenced by no associated organ damage, failure, inflammation, and fetal mortality. Determination of the MTD dose sets the basis for future studies investigating the efficacy of our nanoparticle formulation in a MAR autism mouse model.

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Section: Discussionsupporting

confidence: 82%

“…Legend applies to all graphs. Reference range for nonpregnant C57BL/6j shown in grey (Santos et al, )…”

Section: Resultsmentioning

confidence: 99%

Section: Assessment Of Mar Ldh B Autoantibody Capture In Vitromentioning

confidence: 99%

See 1 more Smart Citation

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Bolandparvaz

Vapniarsky

Harriman

et al. 2020

J Biomedical Materials Res

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“…The obtained haematological and biochemical values of blood and serum of the animals of control group presented similarity, serving as standard for comparison to the other groups. Similar results were also obtained by Santos et al 2016. Anisocytosis and polychromasia were observed in the erythrocytes of all the animals of the infected groups, independent of PVL detection. In addition, the reduced intracellular haemoglobin was detected, as similarly observed in some degenerative anaemia of the bone marrow.…”

Section: Discussionsupporting

confidence: 87%

Pathological profiles of systemic infections by Panton‐Valentine leukocidin‐positive, methicillin‐resistant Staphylococcus aureus strains in a murine model

Silva-Santana

Aguiar-Alves²,

Lenzi-Almeida

et al. 2020

J Appl Microbiol

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Aims Staphylococcus aureus is one of the most common pathogens in hospital environment and community. Panton‐Valentine leukocidin (PVL) production is clinically associated with skin abscesses, soft tissues infections, bacteraemia and sepsis. This study aimed to investigate the effects of the presence of genes lukF/S‐PV coding for PVL, in histological and haematological features during systemic infection, using a Swiss mice experimental model. Methods and Results Experiments were performed using 25 mice distributed into five experimental groups, intravenously inoculated with 50 µl suspensions at density 1·0 × 107 CFU per ml of strains: methicillin‐susceptible (MSSA) and pvl‐negative strains isolated from nasal colonization; MSSA pvl‐positive strains isolated from nasal colonization; methicillin‐resistant (MRSA) and pvl‐positive strains isolated from peripheral blood of a patient with severe pulmonary infection; and a MRSA pvl‐positive strains isolated from a peripheral blood culture of a patient with bacteraemia. Haematological analysis was performed at 24, 48, 72 and 96 h post‐infection. Morphoanatomy and histopathological analyses were performed at 96 h post‐infection. For all S. aureus strains tested, the capability of intravenous dissemination and survival into mice tissues was demonstrated. Inflammatory processes at different levels were related to the presence of pvl genes, and included alterations in the format, size and colour of the organs. Staphylococcus aureus pvl‐positive strains were detected in greater numbers in the organs of the infected animals. Conclusions The pvl‐positive strains isolated from blood cultures were capable to induce the greatest modifications in both haematological and histopathological profiles, and seemed to aggravate the systemic infections. Significance and Impact of the Study These findings are valuable in characterizing infections caused by S. aureus in humans and murine.

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“…The use of animals in scientific research is extremely important, especially in experimental investigations. Animals are used experimentally to enhance understanding of physiological and metabolic mechanisms and also to establish new treatments and improve existing treatments and drugs used in human and veterinary medicine, thus assisting in scientific development …”

Section: Introductionmentioning

confidence: 99%

Hematological reference values and animal welfare parameters of BALB/C‐FMABC (Mus musculus) inoculated with Ehrlich tumor kept in the vivarium at ABC Medical School

Pessini¹,

Souza

Chagas³

et al. 2020

Anim Models and Exp Med

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractBackground: Biochemical and hematological parameters are important tools for assessing the physiological profile of vital organs, and can be recorded to create reference values used for clinical diagnosis of diseases. Many research laboratories lack the means to establish their own set of reference parameters for use in their research, and while there are articles in the literature that discuss laboratory parameters for healthy BALB/c mice, few studies address the evaluation of these parameters in pathological situations, such as in mice inoculated with Ehrlich tumor.Method: BALB/c-FMABC mice previously inoculated with Ehrlich tumor were maintained under appropriate conditions. Blood samples were taken for analysis of hematological parameters using automated and semi-automated equipment to create a set of the animal welfare parameters for evaluation. Result:Results were obtained for all the hematological parameters for all groups analyzed. These showed: statistically significant differences between the initial and final tumor weight; comparable initial tumour volume and weight; an increase in leukocytes in the 7-day group with a characteristic predominance of lymphocytes and neutrophils; statistically significant changes in RDW in the 21-day group and in the welfare parameters in the 28-day group. Conclusion:The study successfully defined and established reference values for hematological and welfare parameters for all groups analyzed. K E Y W O R D S ehrlich tumor carcinoma, experimental animal model, reference value, welfare | 33 PESSINI Et al.

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Hematological and biochemical reference values for C57BL/6, Swiss Webster and BALB/c mice

Cited by 106 publications

References 5 publications

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Pathological profiles of systemic infections by Panton‐Valentine leukocidin‐positive, methicillin‐resistant Staphylococcus aureus strains in a murine model

Hematological reference values and animal welfare parameters of BALB/C‐FMABC (Mus musculus) inoculated with Ehrlich tumor kept in the vivarium at ABC Medical School

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