Hematological and morphometric blood value of four cultured species of economically important tropical foodfish

Bó, Genoefa Amália Dal '; Sampaio, Fernanda Garcia; Losekann, M. E.; Queiroz, J. F. de; Luiz, A. J. B.; Wolf, V.; Gonçalves, Vitória Teodoro; Carra, Maria Lídia

doi:10.1590/1982-0224-20140115

Cited by 31 publications

(26 citation statements)

References 21 publications

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“…The blood data allow responses to the health status of fish subjected to dietary manipulations. The blood values obtained in the present study are within the documented range for the species R. quelen (Dal’Bó, 2015). However, the diet supplemented with Lys only resulted in an increase in the number of erythrocytes and decrease in MCH and MCV.…”

Section: Discussionsupporting

confidence: 83%

Effects of reduced protein level and dietary amino acid supplementation on growth, body composition and intestinal morphometry of silver catfish ( Rhamdia quelen )

Schneider

Durigon

Peixoto³

et al. 2020

Aquac Res

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The reduction in the protein level and the supplementation of lysine (Lys) and methionine (Met) in the diet were evaluated in the performance and intestinal morphometry of silver catfish (Rhamdia quelen). The study was used in a completely randomized design for 63 days, where 320 fish (26.33 ± 0.40 g) were distributed in 20 tanks (250 L, 16 fish each) and fed until the apparent satiety was reached. Five diets were formulated, a positive control [38% of crude protein (CP) (38CP)], a negative control [34% of CP (34CP)] and three test diets [34% of CP supplemented with Lys (34L), 34% of CP supplemented with Met (34M) and 34% of CP supplemented with Lys plus Met (34LM)]. Final weight and condition factor were greater in fish fed the 34LM diet than in those fed the 34CP diet (p < .05). Body lipid deposition was lower in fish fed the 34CP diet than other diets (p = .0002). In the fillet, there was a lower deposition of lipids in fish fed with 34L diet than in fish fed with 34M and 34LM diets (p <.0001). Fish fed the 34LM diet had a higher AA content in the plasma than those fed 34L and 34CP diets (p < .0001). The lower villus height was observed in fish fed the 34L diet compared to the 38CP diet (p = .021). It is possible to reduce 4% of the CP level in silver catfish diets with supplementation of Lys and Met.

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Section: Discussionsupporting

confidence: 83%

Effects of reduced protein level and dietary amino acid supplementation on growth, body composition and intestinal morphometry of silver catfish ( Rhamdia quelen )

Schneider

Durigon

Peixoto³

et al. 2020

Aquac Res

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“…A minor difference in the values of the mentioned morphometric parameters in this study compared to Adili et al [3,10] is likely to be related to the different ovine breed and breeding methods. Breed, age, and sex are known to affect the values of morphometric parameters [11,24]. In our study, the values of RBC length/diameter are almost equal to those of Thamer et al [25] (5.277 ± 0.67 µm), although in a different sheep breed.…”

Section: Discussionsupporting

confidence: 74%

“…Furthermore, changes in RBC morphometric indicators have been detected in certain human [4,7,8] and dog ailments [9]. Morphometric studies of RBCs showed several morphological changes; the most important one is due to age and breed in sheep and goats [10], and also in fish [11]. Buslovskaya et al [12] defined a set of changes of several morphometric and functional parameters of RBCs and white blood cells, characteristic of different adaptive reactions of the chicken organism.…”

Section: Introductionmentioning

confidence: 99%

A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology

Žaja

Vince

Milas

et al. 2019

Animals

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Simple Summary: Haematological tests are an important diagnostic tool for animal diseases. However, little is known beyond the standardly used haematological methods in sheep, especially those in which the detection of sheep red blood cells (RBCs) shape changes is crucial. Our goal is to obtain sheep RBC morphometric parameters as well as RBC subpopulations based on their morphometric parameters. Morphometric parameters of RBC size and shape were determined from stained blood smears using SFORM, a computer-assisted program for automated cell morphometric measurements. Based on their morphometric parameters, three RBC subpopulations were obtained using principal component and cluster analysis: the smallest and most elongated RBCs, the biggest and most rounded ones, and RBCs of average size and shape. When the values of RBC haematological parameters were higher or above the physiological range, a significantly higher proportion of both average size and shape RBCs, as well as the biggest and most rounded RBCs, was obtained. Since they were obtained from healthy animals, these results indicated the importance of determining morphometric parameters of RBCs and the proportion of each RBC subpopulation, which could serve as a basis for future possibilities in the diagnostic interpretation of haematological disorders in sheep, especially those for which the detection of shape changes in ovine RBCs is crucial.Abstract: Data concerning the morphometric parameters of sheep red blood cells (RBCs) obtained using computer-assisted image analysis have not yet been investigated, and there are no data on any analyses of ovine RBC subpopulations based on their morphometric parameters. The aims of this study are to determine the values of RBC haematological and morphometric size and shape parameters, to form groups according to the obtained values of haematological parameters; to determine the differences in RBC morphometric parameters between the formed groups, and to determine RBC subpopulations and their respective proportions in the formed groups. Thirty-six blood samples were collected from the jugular vein of clinically healthy Lika pramenka sheep, aged between 2 and 5 years. Haematological parameters including haemoglobin (HGB), haematocrit (HTC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular Animals 2019, 9, 1130 2 of 15 haemoglobin concentration (MCHC), and RBC distribution width were analysed using a haematology analyser. Haematological parameters were categorized into two groups: those with lower values or values below the physiological range (Groups 1) and groups with higher values or values above the physiological range (Groups 2). Morphometric parameters of RBCs were determined from stained blood smears using SFORM, a computer-assisted program. Significantly higher values of RBC area, outline, convex, minimal and maximal radius, as well as length and breadth were established in Groups 2 compared to Groups 1 of HGB, HCT, MCV, MCH, and MCHC, respectively. Based on the morphometric p...

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“…Both abiotic factors and life history traits can also affect the composition and morphology of fish RBC (Dal’Bó et al, 2015; Kumar, 2016; Yakhnenko et al, 2016). For example, RBC size is inversely related to swimming ability in teleosts (Lay and Baldwin 1999; Witeska 2013), while size of RBCs is highest at intermediate altitude in one lizard species (González-Morales et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…While immature RBC (erythroblasts) are usually rounded, older cells are fusiform (Fijan 2002, Passantino et al 2004). However, the degree of ellipticalness seems to vary among species (Dal’Bó et al, 2015). However, little is known about the physiological implications of the variation in the fusiform shape for species.…”

Section: Introductionmentioning

confidence: 99%

The evolution of red blood cell shape in a continental radiation of fishes

Martins

Franco‐Belussi

Siqueira

et al. 2020

Preprint

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The size and shape of Red Blood Cells (RBC) can provide key information on life history strategies in vertebrates. However, little is known about how RBC shape evolved in response to environmental factors and the role of phylogenetic relationship. Here, we analyzed RBC morphometrics in a continental radiation of fishes testing the hypothesis that phylogenetic relationship determines species occupation of morphospace. We collected blood samples of five specimens of 15 freshwater fish species from six orders and used basic stereological methods to measure cell and nucleus area, perimeter, and diameter, cell and nucleus volume, nucleus:cytoplasm ratio, and shape factor of 50 cells per specimen. Then, we conducted a phylogenetic Principal Components Analysis using a dated phylogeny and built a phylomorphospace. To test if the phylogenetic relationship predicted the phenotypic similarity of species, we calculated multivariate phylogenetic signal. We also estimated the evolution rate of RBC shape for each node and tip using ridge regression. Finally, we tested if the position in the water column influenced RBC shape using a phylogenetic GLS. RBC shape seems to have evolved in a non-stationary way because the distribution pattern of species in the phylomorphospace is independent of the phylogeny. Accordingly, the rate of evolution for shape was highly heterogeneous, with an increase in the genus Pygocentrus. Water column position does not influence RBC shape. In conclusion, RBC shape seem to have evolved in response to multiple selective pressures independent of life history characters.

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Hematological and morphometric blood value of four cultured species of economically important tropical foodfish

Cited by 31 publications

References 21 publications

Effects of reduced protein level and dietary amino acid supplementation on growth, body composition and intestinal morphometry of silver catfish ( Rhamdia quelen )

Effects of reduced protein level and dietary amino acid supplementation on growth, body composition and intestinal morphometry of silver catfish ( Rhamdia quelen )

A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology

The evolution of red blood cell shape in a continental radiation of fishes

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