Hematological changes in long-term perfusion

Brinsfield, Dorothy; Hopf, M. A.; Geering, Ruth B.; Galletti, Pierre M.

doi:10.1152/jappl.1962.17.3.531

Cited by 49 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hemolytic damage depends on magnitude of SS and duration, with the stress threshold for human RBC reported to be 300 Pa when exposed for less than one second [17,28]. SS below the hemolytic threshold may cause sub-hemolytic damage and is evidenced by shortened RBC lifespan [9,32], morphological changes [30], deterioration of RBC deformability [5,14,16,20] and changes in RBC aggregation [12,25].…”

Section: Introductionmentioning

confidence: 99%

Erythrocyte deformability responses to intermittent and continuous subhemolytic shear stress

et al. 2014

View full text Add to dashboard Cite

BACKGROUND: Previous studies have demonstrated that red blood cells (RBC) either lyse or at least experience mechanical damage following prolonged exposure to high shear stress ( 100 Pa). Conversely, prolonged shear stress exposure within the physiological range (5-20 Pa, 300 s) was recently reported to improve RBC deformability. This study investigated the relationships between shear stress and RBC deformability to determine the breakpoint between beneficial vs. detrimental exposure to shear stress (i.e., "subhemolytic threshold"). A second aim of the study was to determine whether the frequency of intermittent application of shear stress influenced the subhemolytic threshold. METHODS: RBC were exposed to various levels of shear stress (0-100 Pa) in a Couette type shearing system for 300 s. RBC deformability was then immediately measured via ektacytometry. Parallel experiments were conducted at the same shear stresses, except the application time differed while keeping constant the total exposure time: shear stress was applied either for 30 s and repeated 10 times (10 × 30 s) or applied for 15 s and repeated 20 times (20 × 15 s). RESULTS: For a range of donors, the subhemolytic threshold with constant shear stress application was between 30-40 Pa. When physiological shear stress was applied in an intermittent manner, more frequent applications tended to improve (i.e., increase) RBC deformability. However, when supra-physiological shear stress was applied, both continuous and intermittent protocols damaged RBC. Changes of RBC mechanical behavior occurred without increases of hemoglobin in the suspending media, thus attesting to the absence of hemolysis. CONCLUSION: Shear stress has a biphasic effect on the mechanical properties of RBC, with the duration and rate of exposure appearing to have minimal impact on the subhemolytic threshold when compared with the magnitude of applied shear stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Erythrocyte deformability responses to intermittent and continuous subhemolytic shear stress

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Some authors [5,7,11,28] have stated that these changes are -to a large extent -independent of the duration of perfusion, but Brinsfield [4], Cooley [6], and Kirby [19] noted a marked initial fall in thrombocytes with an insignificant reduction during the further course of the procedure. This corresponds to our findings in dogs.…”

Section: Discussionmentioning

confidence: 99%

“…(3) Thrombocyte deposits on parts of the apparatus [4,11]. (4) Thrombocyte aggregation and adhesion in the patients' circulato ry system.…”

Section: Discussionmentioning

confidence: 99%

The Pharmacological Influence on Thrombocyte Aggregation in the Extracorporeal Circulation of Dogs

Braun¹,

Braun²,

Hockerts³

1971

Eur Surg Res

View full text Add to dashboard Cite

The purpose of this study was to investigate the effect of a thrombocyte aggregation-inhibitor on the fall in thrombocyte count during extracorporeal circulation in dogs. When extracorporeal circulation had been established, the thrombocyte count observed during the 2-hour experiment with a flow of approximately 20 ml/kg/min showed an average fall of more than 50% of the initial value. This was reduced to 25% by administering a Dipyridamol-derivative (5 mg/kg RA 233, Thomae, Biberach). According to current views loss of thrombocytes during extracorporeal circulation is mainly due to aggregation, although there are other contributary factors such as loss of blood, destruction of thrombocytes, dilution in the system, and deposits of thrombocytes on parts of the heart-lung machine. We suppose that the fall in thrombocytes may be affected favourably by administering an aggregation inhibitor.

show abstract

“…Subhemolytic trauma might be manifest by shortened life span [3], increased trapping in spleen [14], morphological alterations [13], or deterioration in mechanical properties of the cells [2]. Subhemolytic trauma is also determined by both the magnitude and duration of the effective shear stresses [2,9].…”

Section: Mechanical Trauma To Bloodmentioning

confidence: 99%

Red Blood Cell Mechanical Stability

Başkurt¹

2012

ENG

View full text Add to dashboard Cite

It has been well documented that shear forces effective above a certain magnitude under flow conditions causes damage in blood cells. This damage ranges from slight morphological alteration to the destruction of red blood cells (RBC). The hemolytic trauma to RBC can easily be detected by measuring free hemoglobin level in plasma, however there are no standardized protocols to quantitate subhemolytic trauma. Ektacytometry has been used to study the alterations in RBC mechanical properties induced by the application of shear stress at various levels. Additionally, a protocol for measuring the hemolytic threshold as an indicator of subhemolytic damage to RBC has been developed based on ektacytometry. These standardized protocols may find applications in the pre-clinical and clinical evaluation of artificial organs and biomedical devices contacting with blood.

show abstract

Hematological changes in long-term perfusion

Cited by 49 publications

References 0 publications

Erythrocyte deformability responses to intermittent and continuous subhemolytic shear stress

Erythrocyte deformability responses to intermittent and continuous subhemolytic shear stress

The Pharmacological Influence on Thrombocyte Aggregation in the Extracorporeal Circulation of Dogs

Red Blood Cell Mechanical Stability

Contact Info

Product

Resources

About