Hematological Effects of High Dose of Cortisol on the Carp (Cyprinus carpio L.): Cortisol Effect on the Carp Blood

Wojtaszek, J; Dziewulska‐Szwajkowska, Daria; Lozinska-Gabska, M.; Adamowicz, Anna; Dżugaj, Andrzej

doi:10.1006/gcen.2001.7725

Cited by 76 publications

(49 citation statements)

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“…The increase of catecholamines concentration may result in erythrocytes release to the blood stream by spleen contraction (McDonald & Milligan, 1997). Increase of parameters involving red blood cells, such as hemoglobin and hematocrit, has been observed in response to stress (Benfey & Biron, 2000;Wojtaszek et al, 2002;Urbinati et al, 2004). However, vitamin C did not affect hematocrit of matrinxã after air exposure in this study, differing with the findings by Barros et al (2002) in tilapia (Oreochromis niloticus), whose elevation of hematocrit was directly related to the dietary vitamin C levels.…”

contrasting

confidence: 97%

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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The role of vitamin C on physiological responses of matrinxã (Brycon amazonicus) submitted to air exposure was analyzed. Nine hundred fish (70.15 g) were distributed in fifteen 500 l boxes (60 fish.box -1 ) and fed five rations (treatments): Control (no vitamin C); T100 (100 mg); T200 (200 mg); T400 (400 mg) and T800 (800 mg of vitamin C kg.ration -1 ). Each ration was offered to fish of three boxes during 60 days before the stress challenge that consisted of exposing fish to air for two minutes. Samplings were carried out for 5, 15, 30 and 60 minutes after the air exposure. Blood was collected for glucose, cortisol, total protein, sodium, chloride, hematocrit, hemoglobin determination, and white and red cell count. Liver was removed for hepatosomatic index (HSI) calculation and glycogen determination. Vitamin C did not affect the levels of cortisol, chloride, total protein, hemoglobin, leukocytes, hepatic glycogen or HSI in air exposed fish. Blood glucose levels elevation observed 60 minutes after the challenge did not depend on the levels of vitamin C, nor did the drop in serum sodium levels verified 60 minutes after stressor. In general, hematocrit did not change by effect of vitamin C but it was lower at 15 and 30 minutes after the challenge. The number of erythrocytes decreased in fish after 5 minute sampling in all treatments, especially at 30 and 60 minutes. The air exposure evoked alterations in stress indicators of matrinxã, and the vitamin C did not alter the responses. KEY WORDSBrycon amazonicus, capture, stress, ascorbyl -polyphosphate. de glicose, cortisol, proteína total, sódio, cloreto, hematócrito, hemoglobina, contagem Respostas fisiológicas de matrinxã (Brycon amazonicus) arraçoados com diferentes níveis de vitamina C e submetidos à exposição aérea RESUMO Este trabalho avaliou a participação da vitamina C nos indicadoras de estresse no matrinxã (Brycon amazonicus), durante exposição aérea. Novecentos peixes (70,15g) foram distribuídos em caixas de cimento (500 l) e alimentados com os tratamentos: Controle (sem vitamina C); T100 (100 mg); T200 (200 mg); T400 (400 mg); T800 (800 mg de vitamina C kg.ração -1 ). Cada ração foi fornecida a peixes de três caixas por 60 dias antes da aplicação do estressor, que consistiu em suspender os peixes mantendo-os no ar por dois minutos. Cinco, 15, 30 e 60 minutos depois, os peixes anestesiados foram amostrados. Foi coletado sangue para determinação PALAVRAS -CHAVEBrycon amazonicus, captura, estresse, ascorbil polifosfato.

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contrasting

confidence: 97%

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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“…Stress is a biological response of adaptation to adverse conditions and fish respond by activating responses such as increased circulating cortisol and glucose (Wendelaar Bonga, 1997), changes in blood ionic balance (McDonald and Milligan, 1997) and hematological profile (Wojtaszek et al, 2002). Although laboratory studies can be criticized for lack of realism, they allow for a systematic determination of general behavioral and physiological principles of stress response that is not possible in the field.…”

Section: Discussionmentioning

confidence: 99%

“…The results of the hematological assessement of stressed pacu confirmed the low degree of activation of the brain-sympathetic-chromaffin cell axis. Peripherical blood analysis has been used as a diagnosis to assess healthy state in fish and the effect of several stressors on them (Wojtaszek et al, 2002). The most significant effect of catechoalmines release during stress is to enhance blood O 2 transport by increasing the carrying capacity (Wells and Weber, 1990) and by enhancing Hb-O 2 binding affinity (Cossins and Richardson, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…The fish response to such stressors involves a series of physiological changes in an attempt to compensate for the challenge imposed upon it and, thereby cope with the stress. Such changes have been broadly categorized into primary, secondary and tertiary responses including hormonal, metabolic, osmoionic and hematological disturbances and have been used to characterize the degree of stress fish experienced (McDonald and Milligan, 1997;Wendelaar Bonga, 1997;Wojtaszek et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Biological indicators of stress in pacu (Piaractus mesopotamicus) after capture

et al. 2009

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The effects of capture (chasing, netting and air exposure) on cortisol, glucose, chloride, sodium, potassium and calcium concentrations, osmolality, hematocrit, hemoglobin concentration, red blood cells count (RBC) and mean corpuscular volume (MCV) were investigated in pacu (Piaractus mesopotamicus). A total of 132 fish (49.7 ± 11.7 g) were subjected to capture and 3 minutes air exposure and capture and 5 minutes air exposure. Nine fish at each treatment were sampled at 5, 15, 30, 60 minutes and 24 hours after the procedure. Nine undisturbed fish were sacrificed before the handling and used as controls. Capture resulted in a rise in blood cortisol and glucose 30 and 5 minutes, respectively, after both air exposures. Both indicators returned to resting levels 24 hours after capture. In both fish groups, plasma chloride decreased 60 minutes after capture, not recovering the resting levels within 24 hours after, and serum sodium rose at 15 and 30 minutes and recovered the resting levels 24 hours later. There were no significant changes neither in potassium, calcium and osmolality nor in hematocrit, hemoglobin, RBC and MCV as a consequence of capture. The sequential stressors imposed to pacu during capture activated the brain-pituitary-interrenal axis (cortisol and glucose responses) but the activation of the brain-sympathetic-chromaffin cell axis was apparently moderate (ionic and hematological responses).Keywords: capture, air exposure, stress, Piaractus mesopotamicus. Indicadores biológicos de estresse em pacu (Piaractus mesopotamicus) após captura ResumoOs efeitos da captura (perseguição, contenção em puçá e exposição aérea) no perfil sanguíneo do cortisol, glicose, cloreto, sódio, potássio, cálcio e na osmolaridade, hematócrito, hemoglobina, número de células vermelhas (CV) e volume corpuscular médio (VCM) foram investigados no pacu (Piaractus mesopotamicus). Um total de 132 peixes (49,7 ± 11,7 g) foi submetido à captura com 3 ou 5 minutos de exposição aérea. Nove peixes de cada tratamento foram amostrados 5, 15, 30, 60 minutos e 24 horas depois e outros nove peixes foram amostrados antes da captura e considerados controle. A captura resultou em aumento do cortisol e glicose no sangue 30 e 5 minutos depois da captura, respectivamente, independente do tempo de exposição aérea. Ambos os indicadores recuperaram os valores controle em 24 horas. Nos dois grupos de peixes, o cloreto plasmático diminuiu 60 minutos após captura e não recuperou os valores controle, enquanto o sódio sérico aumentou entre 15 e 30 minutos recuperando a condição controle em 24 horas. Não houve alteração significativa nos valores de potássio, cálcio, osmolaridade ou no hematócrito, hemoglobina, CV e VCM como consequência da captura. Os estressores sequenciais aplicados no pacu durante a captura ativaram o eixo cérebro-pituitária-interrenal (respostas do cortisol e glicose), mas a ativação do eixo cérebro-sistema simpático-células cromafins foi aparentemente moderada (respostas iônicas e hematológicas).Palavras-chave: captura, exposiç...

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“…At rest, 'all-fish' GH transgenic carp had similar hematocrit and hemoglobin, but lower MCHC, compared to non-transgenic controls, with all values within normal ranges for carp (Schwaiger et al, 2000;Wojtaszek, et al, 2002;Harikrishnan et al, 2003;Walencik and Witeska, 2007). However, Wang et al (2005) found that 'all-fish' GH transgenic carp had higher hematocirts than age-matched siblings, but transgenics were also significantly heavier and body weight has been shown to affect hematocrit (Houston, 1990(Houston, , 1997.…”

Section: Physiological Response To Exhaustive Exercisementioning

confidence: 99%

The hematological response to exhaustive exercise in ‘all-fish’ growth hormone transgenic common carp (Cyprinus carpio L.)

Wang

et al. 2011

Aquaculture

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Hematological Effects of High Dose of Cortisol on the Carp (Cyprinus carpio L.): Cortisol Effect on the Carp Blood

Cited by 76 publications

References 50 publications

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Biological indicators of stress in pacu (Piaractus mesopotamicus) after capture

The hematological response to exhaustive exercise in ‘all-fish’ growth hormone transgenic common carp (Cyprinus carpio L.)

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