Hematological Humanization of Immune-Deficient Mice

Gergues, Marina; Ayer, Seda; Morelli, Sara S.; Greco, Steven J.; Rameshwar, Pranela

doi:10.1007/978-1-0716-1008-4_14

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…After isolation of CD34+ cells, the presence of T-and B-cells were significantly reduced with almost no NK cells (Figure 5B). These findings indicated that the MPBs stored at optimal conditions could recover CD34+ cells that could be used for humanization of immune deficient mice [8].…”

Section: Recovery Of Immune Cell Subsets Of Cryopreserved Cellsmentioning

confidence: 83%

Cryopreservation of Large Number of Human Hematopoietic Cells – A Model for Research Space with Limited Resources

Ayer,

Greco,

Guiro

et al. 2024

IJTS

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Unlike cell lines, cells from primary human tissues are valuable, requiring storage for long-term use. Large number of primary cells require the most efficient method of shipment, when relevant, and cryopreservation to preserve the viability and function. Shipping of cells could be determined by the specific experimental question. These issues are particularly important for primary cells such as hematopoietic stem cells, which cannot expand in vitro. There is minimum issues when cryopreserving relatively small number of cells from tissues such as umbilical cord blood and bone marrow aspirates. However, cryopreservation of >200 × 109 primary cells comes with challenges to identify the most efficient method for long-term storage. Here we report on processes to achieve efficient cell health and recovery of hematopoietic cells from mobilized peripheral blood cells (MPBs). We also determined overnight shipment of MPBs led to overall outcomes when shipped in the cold, as compared to room temperature. We found that cryopreservation of 50 × 106 cells in 2 mL led to maximum recovery of hematopoietic cell subsets. The implication for these conditions to achieve efficient long-term storage is discussed. These methods will benefit small research laboratories such as those at academic settings and start-up companies with limited resources.

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Section: Recovery Of Immune Cell Subsets Of Cryopreserved Cellsmentioning

confidence: 83%