Hematological Observations of Leukocytes Collected From Bovine Peripheral Blood by an Improved Ammonium Chloride Method

Odajima, Mikiyo; Sonoda, Mitsuo

doi:10.1292/jvms1939.33.295

Cited by 6 publications

(2 citation statements)

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“…!Bovine lymphoid blood cells from LB59 (LB59Ly cells) were obtained by the improved ammoniun chloride solution method of Odajima & Sonoda (1971). The collected cells were then seeded and maintained as monolayers in RPMI 1629 medium supplemented with 20% heat-inactivated (56 °C, 10 min) foetal calf serum (FCSI), 4 ng/ml 8-mercaptoethanol, 100 units/ml penicillin and 100 ~tg/ml streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Establishment and Propagation of a Bovine Leukaemia Virus-producing Cell Line Derived from the Leukocytes of a Leukaemic Cow

Mamoun

Astier

Guillemain

1981

Journal of General Virology

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SUMMARYA cell line (LB59Ly) derived from the leukocytes of a leukaemic cow was established as a monolayer, which spontaneously released large amounts of a retrovirus. This virus was found to be indistinguishable from the bovine leukaemia virus (BLV) produced by the commonly used high-producing heterologous cell line (FLK-BLV). Like the latter, its reverse transcriptase activity was greater in the presence of Mg 2+ cations than in the presence of Mn 2+ cations; its polyacrylamide gel electrophoresis pattern showed the presence of gp51, p24, p 15, p 12 and p 10, and the antigenicity of the two major proteins completely cross-reacted with those of BLV from FLK-BLV cells. The virus was infectious and induced early and late polykaryocytosis, the specificity of which was demonstrated by use of specific anti-BLV sera.

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Section: Methodsmentioning

confidence: 99%

Establishment and Propagation of a Bovine Leukaemia Virus-producing Cell Line Derived from the Leukocytes of a Leukaemic Cow

Mamoun

Astier

Guillemain

1981

Journal of General Virology

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“…Collection of large numbers of white blood cells from the cow-J 5 was performed by the improved ammonium chloride solution method as described by Odajima and Sonoda (1971). Blood containing 2 mg. ethylenedianiinetetraacetate (EDTA) per ml.…”

Section: Leukocyte Preparationmentioning

confidence: 99%

Studies on Bovine Leukemia

Ressang

Mastenbroek

Quak

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe B

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Summary The fluorescent antibody technique and the immunodiffusion test were used for the serological detection of type C virus found in one bovine and two sheep established cell lines. The electron microscopic demonstration of type C virus particles supported by uridine labelling suggested that a virus specific antigen underlay the FA reaction and the reaction of identity in the IDT. Preliminary FA tests employing various antisera against mammalian oncornaviruses may indicate that bovine leukemia virus represents antigenically a unique group. Zusammenfassung Untersuchungen über die Rinderleukose I. Gewinnung von C‐Typ virusproduzierenden Zell‐Linien Leukozyten einer Leukose‐kranken Kuh und Lymphknoten‐explantate von zwei Leukose‐infizierten Schafen dienten zur Gewinnung permanenter Zell‐Linien. In diesen Zellkulturen konnten C‐Typ Virus und Virusantigen elektronenoptisch, fluoreszenzserologisch, mittels Immunodiffusion und durch Markierung mit 3H‐Uridin nachgewiesen werden. Vergleichende fluoreszenzserologische Untersuchungen mit Seren gegen eine Reihe von Oncornaviren anderer Säugetiere deuten darauf hin, daß das Virus der Rinderleukose eine antigen selbständige Untergruppe der Oncornaviren darstellt. Résumé Recherches sur la leucose bovine I. Obtention des lignées cellulaires produisant du virus type C Des leucocytes d'une vache atteinte de leucose et des explantats de ganglions de deux moustons infectés de leucose ont servi à obtenir les lignées cellulaires permanentes. On a pu mettre en évidence dans ces cultures cellulaires un virus du type C et un antigène viral au microscope électronique, par l'immunofluorescence, au moyen de l'immunodiffusion et par marquage avec 3H‐uridine. Des recherches comparées en fluorescence avec des antisérums d'une série d'oncornavirus d'autres mammifères ont montré que le virus de la leucose bovine représente un sous‐groupe antigèniquement unique. Resumen Estudios sobre la leucosis bovina I. Obtención de líneas celulares que producen el virus tipo C Los leucocitos de una vaca leucótica y las explantaciones ganglionares de dos ovejas infectadas con leucosis se utilizaron para obtener líneas celulares permanentes. En estos cultivos celulares se identificaron partículas virales del tipo C con la ayuda del microscopio electrónico y del marcaje con uridina‐3H. Se detectaron también antígenos virales con las técnicas de immunofluorescencia e imunodifusión. Los estudios fluorescenteserológicos comparativos realizados con sueros contra una serie de virus oncorna de otros mamíferos parecen indicar que el virus de la leucosis pertenece a un subgrupo antigénicamente independiente de los virus oncorna.

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Early polykaryocytosis inhibition: A simple quantitative in vitro assay for the detection of bovine leukemia virus infection in cattle

Guillemain¹,

Mamoun²,

Lévy

et al. 1978

European Journal of Cancer (1965)

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Hematological Observations of Leukocytes Collected From Bovine Peripheral Blood by an Improved Ammonium Chloride Method

Cited by 6 publications

References 0 publications

Establishment and Propagation of a Bovine Leukaemia Virus-producing Cell Line Derived from the Leukocytes of a Leukaemic Cow

Establishment and Propagation of a Bovine Leukaemia Virus-producing Cell Line Derived from the Leukocytes of a Leukaemic Cow

Studies on Bovine Leukemia

Early polykaryocytosis inhibition: A simple quantitative in vitro assay for the detection of bovine leukemia virus infection in cattle

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