Hematological values for free-living great fruit-eating bats, Artibeus lituratus (Chiroptera: Phyllostomidae)

Amaral, Maria Alice Zacarias do; Tavares, Jonatas Amorim; Fernandes, Priscila do Amaral; Alves, Bruno; Neto, Sócrates Fraga de Costa; Lacorte, Caroline; Borges, Mylena; Bonna, Isabel Cristina Fábregas; Andreazzi, Cecilia S.; Moratelli, Ricardo

doi:10.11606/issn.1678-4456.bjvras.2020.168582

Cited by 2 publications

(3 citation statements)

References 29 publications

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“…Present study agrees with (Kuzel, 2020) who reported that male great fruiteating bat (Artibeus lituratus) higher than female in WBC, RBC, HB, Neutrophil, Lymphocyte and monocytes but male Artibeus lituratus less female in Platelet count. And agree with (Olayemi et al, 2006) who reported that male African Fruit Bat (Eidolon Helvum) higher than female in RBC, HCT, HB, MCV, WBC, Neutrophil, Esinophil and monocyte.…”

Section: Resultssupporting

confidence: 92%

Effect of sex and age on some Morphometric, Hematological and Biochemical Parameters in Egyptian Fruit Bat (Rousettus aegyptiacus)

Eldeify,

Ali,

Fawaz

et al. 2023

SVU-International Journal of Veterinary Sciences

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Present study on Egyptian Fruit Bat (Rousettus aegyptiacus) conduct on 60 apparently healthy bats (19 young, 36 adults and 5 neonate) and designed to investigate data base in this species, bat's morphometric measurements needed to determine all bat's external characters which help in identification of different species and different age or sex. morphometric parameters measured by ruler, show significant difference between different age and no effect of sex, overall mean of body weight 111.1±34.29 g, Forearm 8.74±0.85 cm, body length 14.26±1.58 cm and Wingspan 61.18±6.41 cm. Evaluation of hematological and biochemical parameters of animals are very important to diagnosis diseases and to determine a requirements of nutrition to them. hematological parameters measured by Hemocytometer show significant difference between different age in RBC, MCV, Eosinophil and no effect of sex, overall mean of RBC 7.19±2.56 10 12 /l, WBC 6.87±3.96 10 9 /l, HB 13.99±1.96 g/dl and Platelets 199.3±118.9 10 9 /l. biochemical parameters measured by Spectrophotometer show significant difference between different age in triglyceride and between different sex in total calcium and cholesterol, overall mean of AST 219.5±191.2 IU/l, ALT 115.6±77.68 IU/l, Total calcium 1.85±0.40 mmol/l, Urea 3.98±3.48 mmol/l, Cholesterol 0.26±0.15 mmol/l, Glucose 5.37±1.70 mmol/l.

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Section: Resultssupporting

confidence: 92%

Effect of sex and age on some Morphometric, Hematological and Biochemical Parameters in Egyptian Fruit Bat (Rousettus aegyptiacus)

Eldeify,

Ali,

Fawaz

et al. 2023

SVU-International Journal of Veterinary Sciences

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“…The most relevant results in the red blood cell count show some bats with decreases in hemoglobin, in addition all individuals presented some low erythrocyte indices from day 0 to day 21 after inoculation, there being a clear normochromic microcytic anemia in M2 on day 14 post-inoculation and M9 presented a marginal erythrocytosis on day 21 postinoculation. The most relevant results in the white blood cell count compared to the values given for Artibeus bats (4.7 ± 2.4 × 10 9 /L) (31-34) (Table 2). show leukocytosis with lymphocytosis and monocytosis in individuals M6 and M8, in addition to lymphocytosis in M9 and leukocytosis with lymphocytosis in M11.…”

Section: Resultsmentioning

confidence: 96%

“…Next, blood samples were divided for molecular and serological ZIKV detection and whole blood for complete blood count (CBC). The CBC was made in a VetScan HM5 device previously calibrated for Phyllostomid bat leucocytes using the reference previous reports (31-34). Differential counts WBC were made also in blood smears stained with Giemsa dye following standard protocols.…”

Section: Methodsmentioning

confidence: 99%

Experimental infection of Artibeus lituratus bats and no detection of Zika virus in neotropical bats from French Guyana, Peru, and Costa Rica, suggest a limited role of bats in Zika transmission

Aguilar-Setién

Salas-Rojas

Romero

et al. 2022

Preprint

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Bats are important natural reservoir hosts of a diverse range of viruses that can be transmitted to humans and have been suggested that could play an important role in the Zika virus (ZIKV) transmission cycle. However, the exact role of these animals as reservoirs for Flaviviruses is still controversial. To further expand our understanding of the role of bats in the ZIKV transmission cycle in Latin America, we carried an experimental infection in wild-caught Artibeus bats and sampled several free-living neotropical bats over three countries of the region. Experimental ZIKV infection was made in free-ranging adult bats (4 females and 5 males). The most relevant gross findings were hemorrhages in the bladder, stomach and patagium. Significant histological findings included inflammatory infiltrate consisting of a predominance of neutrophils and lymphocytes, in addition to degeneration in the reproductive tract of males and females. This suggests that bat reproduction might be at some level affected by ZIKV. Leukopenia was also observed in some inoculated animals. Hemorrhages, genital alterations, and leukopenia are suggestive to be caused by ZIKV, however, since these are wild-caught bats, we can not exclude other agents. Excretion of ZIKV by qPCR was detected (low titles) in only two urine samples in two inoculated animals. All other animals and tissues tested negative. Finally, no virus-neutralizing Abs were found in any animal. To determine ZIKV infection in nature, a total of 2056 bats were blood sampled for ZIKV detection by qPCR. Most of the sampled individuals belonged to the genus Pteronotus sp. (23%), followed by the species Carollia sp. (17%); Anoura sp. (14%), and Molossus sp. (13.7 %). No sample of any tested species resulted positive to ZIKV by qPCR.These results together suggest that bats are not efficient amplifiers or reservoirs of ZIKV and may not have an important role in ZIKV transmission dynamics.Author summaryIn previous works made in 2008-2009, we have found the presence of antibodies against Flaviviruses and viral RNA has been detected in Neotropical chiropterans of Mexico, which led us to support the hypothesis that these animals could be reservoirs of Flaviviruses. As controversial opinions have been exposed, and based on a previous (2019) experimental ZIKV infection made in Colorado State University using adult Artibeus males from a captive colony, in this work we also experimentally infected adult Artibeus males complementarily adding females and using free-living animals instead of laboratory bats. We also monitored a diverse range of natural bat populations in Latin America for the presence of viral RNA against ZIKV in blood. A plaque reduction seroneutralization test was used for the detection of antibodies against ZIKV. Similar to the previous work, we found histopathological alteration in male testicles but also in ovaries and oviducts of females, as well as gliosis and multifocal necrosis in pyramidal neurons and Purkinge cells of inoculated animals. Only two urine samples from inoculated animals showed viral RNA. Additionally, leukopenia and lymphoid follicular splenic hyperplasia were evidenced. Differing to what was reported, no neutralizing antibodies against ZIKV were detected in any sample. Viral RNA within the blood was not present in any of the 2056 bat samples collected in French Guyana, Peru and Costa Rica and proceeding from 33 bat genera. These results together suggest that bats are not efficient amplifiers or reservoirs of ZIKV and might not have an important role on ZIKV transmission dynamics.

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Hematological values for free-living great fruit-eating bats, Artibeus lituratus (Chiroptera: Phyllostomidae)

Cited by 2 publications

References 29 publications

Effect of sex and age on some Morphometric, Hematological and Biochemical Parameters in Egyptian Fruit Bat (Rousettus aegyptiacus)

Effect of sex and age on some Morphometric, Hematological and Biochemical Parameters in Egyptian Fruit Bat (Rousettus aegyptiacus)

Experimental infection of Artibeus lituratus bats and no detection of Zika virus in neotropical bats from French Guyana, Peru, and Costa Rica, suggest a limited role of bats in Zika transmission

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