Hematology and Clinical Chemistry Parameters in the Cat (Felis domesticus)

BackgroundThere are not reported regarding the protocols for obtaining platelet concentrates (PC) in cats for medical purposes. The objectives of this study were: 1) to describe a manual method for producing two kinds of PC in cats (PC-A and PC-B), 2) to describe the cellular population of the PC, 3) to measure and compare the effect of calcium gluconate (CG) and bovine thrombin (BT) on the temporal release of transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor type BB (PDGF-BB) at 3 and 12 hours post-activation and 4) to establish correlations between the cellular population of both PCs and the concentration of growth factors (GF). Blood samples were taken from 16 cats for complete blood count, plasma collection and PC preparation. The PC were arbitrarily divided into two fractions, specifically, PC-A (lower fraction) and PC-B (upper fraction).ResultsThe platelet counts were significantly different (P<0.05) between the PC and whole blood but not between the PC fractions. The TGF-β1 concentration efficiencies for PC-A and PC-B activated with CG were 42.86% and 46.54%, and activated with BT were 42.88% and 54.64%, respectively. The PDGF-BB concentration efficiencies for PC-A and PC-B activated with CG were 61.36% and 60.61%, and activated with BT were 65.64% and 72.12%, respectively. The temporal release of GFs showed no statistically significant difference (P>0.05) between the activating substances at the time or for any PC fraction.ConclusionsWhatever the activation means, these preparations of cat PC provide significant concentrations of platelets and GFs for possible clinical or experimental use.

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“…Cats with a basal platelet count less than 300 ×10 3 PLT/μL were not included (normal basal minimum count) [55]. …”

Section: Methodsmentioning

confidence: 99%

Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates

et al. 2012

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“…Differences in hematologic values exist between venipuncture sites in other species, including rats, tortoises, and dogs. [8][9][10] However, it is most typical that there are no significant differences in values for blood samples collected from various venipuncture sites, as indicated in studies [11][12][13] in rhinoceri, cats, and chameleons. In rats and dogs, the differences were attributed to local tissue damage from samples collected from peripheral vascular sites.…”

Section: Discussionmentioning

confidence: 99%

Differences in hematocrit of blood samples obtained from two venipuncture sites in sharks

Mylniczenko¹,

Curtis²,

Wilborn³

et al. 2006

Journal of the American Veterinary Medical Association

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T he field of elasmobranch medicine is in its infancy, and there is a paucity of information on hematologic values 1-4 despite the growing need for veterinary care of cap-tive sharks. Sites for collection of blood samples in sharks are typically chosen in accordance with the method of animal handling. When a shark is large or difficult to manually restrain, it is most convenient to obtain a blood sample from the dorsal sinus, an area located at the caudal aspect at the base of the dorsal fin. This method allows handlers to maintain the shark in ventral recumbency. When a shark is easily restrained or anesthetized, blood collection from the caudal tail vein or artery is usually chosen because the shark can be placed in dorsal recumbency, which facilitates access to the vessels. In the authors' clinical experience, Hcts differ in samples collected from these 2 sites for use in routine health screening of captive shark populations. To assess the importance of this observation, a prospective study was initiated that used both captive habituated sharks and captured free-ranging (wild) sharks.Sharks can be classified into metabolic categories on the basis of their method of respiration. Pelagic sharks (ie, open-water sharks) rely on ram ventilation. These sharks ensure ventilation by forcing water over their gills and, hence, are obligated to swim constantly. Conversely, nonpelagic sharks (ie, reef-oriented species of sharks) are capable of pumping water over their gills and can rest on the ocean bottom for extended periods. The nonpelagic species have a higher tolerance for anaerobic situations than do the pelagic sharks. 5,6 The Hct in tuna, a high-energy, ram-ventilating teleost, is considerably higher than the Hct in most other teleosts because tuna have a high requirement for oxygen. 7 Therefore, we elected to assess whether similar differences existed in pelagic and nonpelagic sharks. Materials and MethodsAnimals-Blood samples were collected from 47 adult sharks. The sharks comprised 2 groups (32 captive habituated sharks and 15 captured free-ranging wild sharks). Sharks were considered adults on the basis of secondary sex characteristics; standard and total length measurements; and, when possible, body weight.Collection of blood samples-Captive habituated adult sharks were acclimated to captive conditions for at least 1 year, and all were handled or anesthetized during that time period. Samples were obtained twice from some sharks. Animals included 4 brown sharks (Carcharhinus plumbeus), 13 blacktip reef sharks (Carcharhinus melanopterus), 3 wobegong sharks (Orectolobus japonicus), 4 zebra sharks (Stegastoma fasciatum), and 8 whitetip reef sharks (Triaenodon obesus). All sharks were considered healthy on the basis of results of physical examination, routine hematologic tests, and serum biochemical analysis, as determined by use of in-house reference ranges.Objective-To evaluate differences in Hct between 2 venipuncture sites in captive and free-ranging sharks. Animals-32 healthy adult captive sharks (Carcharhinu...

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“…[8][9][10] However, it is most typical that there are no significant differences in values for blood samples collected from various venipuncture sites, as indicated in studies [11][12][13] in rhinoceri, cats, and chameleons. In rats and dogs, the differences were attributed to local tissue damage from samples collected from peripheral vascular sites.…”

Section: Discussionmentioning

confidence: 99%

“…Differences in Hct between venipuncture sites in turtles and other reptiles have been attributed to lymph contamination as a result of the close proximity of lymphatics to blood vasculature. 12,14 In sharks, several sites have been used for antemortem blood collection, including the caudal tail vessels, heart, and cranial dorsal fin sinus. Cardiac collection is not recommended because of the anatomic barriers surrounding the heart and potential morbidity that may result when cardiac tissue is damaged.…”

Section: Discussionmentioning

confidence: 99%

Differences in hematocrit of blood samples obtained from two venipuncture sites in sharks

Mylniczenko

Curtis²,

Wilborn³

et al. 2006

ajvr

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Hct values were significantly lower in blood samples collected from the cranial dorsal fin sinus compared with values for samples collected from the caudal tail artery. It is important to recognize this difference when evaluating hematologic variables in sharks and when establishing reference ranges for Hcts for shark populations. Sharks that were more active and relied on aerobic metabolism had higher Hct values than did anaerobic-tolerant sharks, and the difference in Hct values between venipuncture sites was more pronounced.

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Hematology and Clinical Chemistry Parameters in the Cat (Felis domesticus)

Cited by 21 publications

References 5 publications

Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates

Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates

Differences in hematocrit of blood samples obtained from two venipuncture sites in sharks

Differences in hematocrit of blood samples obtained from two venipuncture sites in sharks

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