Hematology and Clinical Chemistry Reference Intervals for Inbred Strain 13/n Guinea Pigs (<i>Cavia Porcellus</i> )

Genzer, Sarah C.; Huynh, Thanhthao; Coleman-McCray, JoAnn D.; Harmon, Jessica R.; Welch, Stephen R.; Spengler, Jessica R.

doi:10.30802/aalas-jaalas-18-000118

Cited by 24 publications

(24 citation statements)

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“…Waner et al compared hematologic and clinical chemistry parameters of 1 month old, male haired (n = 10) and hairless (n = 12) Dunkin Hartley guinea pigs [4], but did not include a high enough sample size to establish reference intervals as put forth by the ASVCP guidelines [3]. Prior studies have determined reference intervals in Weiser-Maples [5] and strain 13 [6] guinea pigs. However, there are likely differences in hematology and biochemistry parameters between strains of guinea pigs, similar to other laboratory rodents [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus)

et al. 2021

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The Dunkin Hartley is the most common guinea pig strain used in biomedical research, particularly for studies of asthma, allergy, infectious disease, reproduction, and osteoarthritis. Minimally invasive blood tests, such as complete blood counts and serum biochemistry profiles, are often collected for diagnostics and laboratory analyses. However, reference intervals for these assays have not yet been well-documented in this strain. The purpose of this study was to establish reference intervals for hematologic and biochemical parameters of Dunkin Hartley guinea pigs and determine age- and sex-related differences. Hematologic and biochemical parameters were retrospectively obtained from 145 male and 68 female guinea pigs between 2 and 15 months of age. All blood parameters were analyzed by a veterinary clinical pathology laboratory. Reference intervals were established according to the American Society for Veterinary Clinical Pathology guidelines. Age- and sex-related differences were determined using unpaired t-tests or nonparametric Mann-Whitney tests. Hematocrit, red blood cell distribution width, mean platelet volume, white blood cell count, heterophils, monocytes, eosinophils, glucose, blood urea nitrogen, creatinine, calcium, magnesium, total protein, albumin, globulin, cholesterol, aspartate aminotransferase, gamma glutamyl transferase, and bicarbonate increased with age. Mean corpuscular hemoglobin concentration, cellular hemoglobin concentration mean, platelets, lymphocytes, phosphorus, albumin/globulin ratio, alkaline phosphatase, anion gap, and calculated osmolality decreased with age. Males had higher hemoglobin, hematocrit, red blood cell count, mean corpuscular hemoglobin concentration, white blood cell count, heterophils, Foa-Kurloff cells, alanine aminotransferase, and bicarbonate and lower mean corpuscular volume, red blood cell distribution width, platelets, mean platelet volume, eosinophils, total protein, albumin, globulin, cholesterol, potassium, anion gap, calculated osmolality, and iron compared to females. Establishing age and sex differences in hematologic and biochemical parameters of Dunkin Hartley guinea pigs provides valuable insight into their physiology to better evaluate diagnostics and experimental results.

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Section: Introductionmentioning

confidence: 99%

Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus)

et al. 2021

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“…For the comparative analysis, we studied the hematological and serum biochemical parameters of guinea pigs. Based on the hematological and serum biochemical data of an inbred strain, 13/N guinea pigs were divided into the following age groups -juveniles (0-150 days), adults (151-900 days) and geriatric adults (older than 900 days) [28]. Our selection for the experimental study was 48-month-old guinea pigs.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

Aldayarov

Jumabekova

Kurmanbekova

et al. 2021

Preprint

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Background: Kuchala (Arum korolkowii Regel) is a medicinal plant often used in folk medicine in the Kyrgyz Republic. As a medicinal raw material, the tuber’s tincture is used in small doses to increase human sexual potency. However, there is no scientific evidence in support of the medicinal effects of kuchala. For these reasons, we decided to study the pharmacological effect of kuchala tuber tincture on the sexual potency of adult male guinea pigs. We analyzed the effect of kuchala at the age of ±48-months, in 12 male guinea pigs. A preparation of 10% tuber tincture of kuchala in 70% ethanol was administered perorally to the male guinea pigs in the form of a once-daily dose of 150 µl for 30 days. The study data were obtained by ethological, hematological and serum biochemistry, gross anatomical, histological and statistical methods.Results: The hematological and serum biochemistry parameters were significantly different between the control and the experimental group. The neutrophils’ percentage in the experimental group was significantly lower (dР < 0.001) than in the control group. On the other hand, the lymphocyte counts were significantly higher in the experimental group (dР < 0.001). The RBC counts, Hgb, Hct, MCH and MCHC were significantly higher in the experimental group (dР < 0.001; dР < 0.001; cР < 0.01; dР < 0.001; dР < 0.001 respectively) than in the control group. In contrast, the color indicator and the mean platelet volume were higher (bР < 0.05) and significantly higher (dР < 0.001) respectively in the control group than in the experimental group. The ALT and AST levels were lower in the experimental than in the control group (both dР < 0,001). The testosterone concentration in serum was much higher (dР < 0,001) in the experimental group. Microscopically, some structural damages were found in the liver of the experimental animals indicating a metabolic disorder. However, the testes showed an improvement in spermatogenesis in the experimental compared with the control group.Conclusions: The 10% kuchala tuber tincture in 70% ethanol has a positive effect in terms of improving the sexual potency of adult guinea pigs by increasing the production of testosterone and increasing spermatogenesis.

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“…For the comparative analysis, we studied the hematological and serum biochemical parameters of guinea pigs. According to hematological and serum biochemical data of inbred strain, 13/N guinea pigs were divided into the following age groups -the juveniles (0-150 days), adults (151-900 days) and geriatric adults (older than 900 days) [32]. Our selection for the experimental study guinea pigs at the age of 48 months, although from a different breed, are fully meets to our goal for choose of old animals.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

Aldayrov

Jumabekova

Kurmanbekova

et al. 2020

Preprint

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BackgroundKuchala (Arum korolkowii Regel) is one of the medicinal plants often used in folk medicine in the Kyrgyz Republic. As a medicinal raw material, tubers tincture used in small doses to increase human sexual potency. However, there is no scientific evidence proving the medicinal effects of kuchala. For these reasons, we decided to study the effect of kuchala tuber tincture on the sexual potency of adult male guinea pigs.MethodsWe analyzed the effect of kuchala at age ±48-month-old, in 12 male guinea pigs. Prepared 10% tuber tincture of kuchala in 70% ethanol was administered perorally a once-daily dose of 150 µl for 30 days for the male guinea pigs. These study data were obtained by the ethological, hematological and serum biochemistry, gross anatomical, histological and statistical methods.ResultsThe hematological and serum biochemistry parameters were significantly different between control and experimental group. Neutrophils’ percentage in experimental group was significantly lower (dР < 0.001) than in control group. Lymphocyte counts on the contrary were significantly higher in experimental group (dР < 0.001). RBC counts, Hgb, Hct, MCH and MCHC were significantly higher in the experimental group (dР < 0.001; dР < 0.001; cР < 0.01; dР < 0.001; dР < 0.001 respectively) than control group. The color indicator and mean platelet volume contrary was higher (bР < 0.05) and significantly higher (dР < 0.001) in control animals than in the experimental. ALT and AST levels were lower in experimental than control group (both dР < 0,001). The testosterone concentration in serum was much higher (dР < 0,001) in the experimental group. Microscopically was found minor structural damage in the liver tissue that indicates a metabolic disorder. However, the testes showed an improvement in spermatogenesis in the experimental if compared with the control group.ConclusionThe 10% kuchala tuber tincture in 70% ethanol has a positive effect to improve the sexual potency of old guinea pigs by increasing the production of testosterone and increasing spermatogenesis.

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Hematology and Clinical Chemistry Reference Intervals for Inbred Strain 13/n Guinea Pigs (Cavia Porcellus )

Cited by 24 publications

References 24 publications

Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus)

Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus)

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

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