Hematology from embryo to adult in the bobwhite quail (Colinus virginianus): Differential effects in the adult of clutch, sex and hypoxic incubation

Flores-Santin, Josele; Antich, Maria Rojas; Tazawa, Hiroshi; Burggren, Warren W.

doi:10.1016/j.cbpa.2018.01.005

Cited by 7 publications

(12 citation statements)

References 45 publications

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“…None of the blood chemistry or hematological variables measured in the offspring population varied significantly between experimental groups (Table 1). With exception of PO 2 and PCO 2 values that were associated with Tb of the hatchling, none of the variables were associated with the covariates for any of the hematological variables (the random effect of week of collection was not statistically significant, P > 0.05), with the exception of Osm (P < 0.002), Noteworthy is the fact that the values obtained in this study for the king quail fall within the ranges previously reported for hatchlings of the bobwhite quail (Colinus virginianus), the common quail (Coturnix coturnix), and the chicken (Gallus gallus; Burggren and Elmonoufy, 2017;Flores-Santin et al, 2018;Amaral-Silva et al, 2020). Similarly, none of the organ masses significantly differed between hatchlings from the Control, Low or High oil-exposed parental groups (Table 2) and, for none of the organ masses when the random effect of week of collection was included, with the exception of gut mass (P < 0.002).…”

Section: Blood Chemistry and Hematological Variables And Organ Massessupporting

confidence: 61%

Dietary Exposure to Low Levels of Crude Oil Affects Physiological and Morphological Phenotype in Adults and Their Eggs and Hatchlings of the King Quail (Coturnix chinensis)

et al. 2021

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Despite the current knowledge of the devastating effects of external exposure to crude oil on animal mortality, the study of developmental, transgenerational effects of such exposure has received little attention. We used the king quail as an animal model to determine if chronic dietary exposure to crude oil in a parental population would affect morpho-physiological phenotypic variables in their immediate offspring generation. Adult quail were separated into three groups: (1) Control, and two experimental groups dietarily exposed for at least 3 weeks to (2) Low (800 PAH ng/g food), or (3) High (2,400 PAH ng/g food) levels of crude oil. To determine the parental influence on their offspring, we measured metabolic and respiratory physiology in exposed parents and in their non-exposed eggs and hatchlings. Body mass and numerous metabolic (e.g., O2 consumption, CO2 production) and respiratory (e.g., ventilation frequency and volume) variables did not vary between control and oil exposed parental groups. In contrast, blood PO2, PCO2, and SO2 varied among parental groups. Notably, water loss though the eggshell was increased in eggs from High oil level exposed parents. Respiratory variables of hatchlings did not vary between populations, but hatchlings obtained from High oil-exposed parents exhibited lower capacities to maintain body temperature while exposed to a cooling protocol in comparison to hatchlings from Low- and Control-derived parents. The present study demonstrates that parental exposure to crude oil via diet impacts some aspects of physiological performance of the subsequent first (F1) generation.

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Section: Blood Chemistry and Hematological Variables And Organ Massessupporting

confidence: 61%

Dietary Exposure to Low Levels of Crude Oil Affects Physiological and Morphological Phenotype in Adults and Their Eggs and Hatchlings of the King Quail (Coturnix chinensis)

et al. 2021

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“…After light anesthesia using 1.5% of halothane in ambient air via face mask for <2 min (Flores‐Santin et al 2018), 300 µL of arterial blood was collected from the ulnar artery using a 1‐mL heparinized syringe and transferred to a 1.5‐mL vial. Immediately after collection, 120 µL of blood was transferred to a blood gas analyzer (ABL TH 5 Radiometer; Medical A/S) for determination of partial pressure of O 2 (PO 2 ), partial pressure of CO 2 (PCO 2 ), pH, and bicarbonate concentration ([HCO 3 − ]).…”

Section: Methodsmentioning

confidence: 99%

Metabolic and Hematological Responses to Endotoxin‐Induced Inflammation in Chicks Experiencing Embryonic 2,3,7,8‐Tetrachlorodibenzodioxin Exposure

Amaral-Silva

Tazawa

Bícego

et al. 2020

Enviro Toxic and Chemistry

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Dioxin exposure during bird embryonic development disrupts immunity as well as mechanisms involved in energy metabolism, potentially affecting negatively acute-phase responses to pathogens. Thus, we hypothesized that embryonic exposure to 2,3,7,8-tetrachlorodibenzodioxin (TCDD) changes the metabolism and blood physiology of domestic chicks, affecting their physiological competence for responding to immune challenges. To test this hypothesis, we injected doses of 0, 1.5, and 3 ng TCDD/egg (based on survival experiments) on embryonic day 4 and then measured O 2 consumption and CO 2 production for metabolic rate, ventilation, and body temperature (T B) in 5-d-old chicks. Then, chicks were injected with lipopolysaccharide (LPS, endotoxin) or saline prior to repeating the physiological measurements. A second chick group exposed to identical TCDD and LPS treatments had blood partial pressure of oxygen, partial pressure of carbon dioxide, pH, bicarbonate concentration, lactate concentration, osmolality, hemoglobin concentration, red blood cell concentration, and hematocrit, as well as T B , analyzed at 1 and 5 h after LPS injection. Metabolism in chicks embryonically exposed to 1.5 and 3 ng TCDD/egg was up to 37% higher, whereas body mass of chicks exposed to 3 ng TCDD/egg was approximately 6% lower. Chicks embryonically exposed to 3 ng TCDD/egg challenged with LPS showed a relative persistent hypometabolism accompanied by elimination of the normal hematological and osmotic responses to LPS. We conclude that embryonic exposure to TCDD affects posthatching metabolism as well as impairs metabolic, hematological, and osmotic responses to LPS.

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“…The experimental setup of the study is ‘translated’ into the model as follows: individuals reach puberty sometime before the start of the experiment, presumably when they are roughly 100 days old (assumption based on Flores-Santin et al , 2018 ). After this, we assume they immediately start investing energy into the reproduction buffer ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$E_R$\end{document} ) at a rate \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$\dot {p}_R$\end{document} ( Kooijman, 2010 ), but this does not yet result in laid eggs (birds did not start laying eggs in Flores-Santin et al , 2018 ). Based on the data, we set the start of ingestion up-regulation as being 25 days before the start of egg-laying; during this period, we assume that the reproductive organs prepare (enlarge) ( Cain & Lien, 1985 , Vezina & Salvante, 2010 ), after which eggs are released.…”

Section: Methodsmentioning

confidence: 99%

Energetic basis for bird ontogeny and egg-laying applied to the bobwhite quail

Marn

Lika

Augustine

et al. 2022

Conservation Physiology

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Birds build up their reproductive system and undergo major tissue remodeling for each reproductive season. Energetic specifics of this process are still not completely clear, despite the increasing interest. We focused on the bobwhite quail — one of the most intensely studied species due to commercial and conservation interest — to elucidate the energy fluxes associated with reproduction, including the fate of the extra assimilates ingested prior to and during reproduction. We used the standard Dynamic Energy Budget model, which is a mechanistic process-based model capable of fully specifying and predicting the life cycle of the bobwhite quail: its growth, maturation and reproduction. We expanded the standard model with an explicit egg-laying module and formulated and tested two hypotheses for energy allocation of extra assimilates associated with reproduction: Hypothesis 1, that the energy and nutrients are used directly for egg production; and Hypothesis 2, that the energy is mostly spent fueling the increased metabolic costs incurred by building up and maintaining the reproductive system and, subsequently, by egg-laying itself. Our results suggest that Hypothesis 2 is the more likely energy pathway. Model predictions capture well the whole ontogeny of a generalized northern bobwhite quail and are able to reproduce most of the data variability via variability in (i) egg size, (ii) egg-laying rate and (iii) inter-individual physiological variability modeled via the zoom factor, i.e. assimilation potential. Reliable models with a capacity to predict physiological responses of individuals are relevant not only for experimental setups studying effects of various natural and anthropogenic pressures on the quail as a bird model organism, but also for wild quail management and conservation. The model is, with minor modifications, applicable to other species of interest, making it a most valuable tool in the emerging field of conservation physiology.

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Hematology from embryo to adult in the bobwhite quail (Colinus virginianus): Differential effects in the adult of clutch, sex and hypoxic incubation

Cited by 7 publications

References 45 publications

Dietary Exposure to Low Levels of Crude Oil Affects Physiological and Morphological Phenotype in Adults and Their Eggs and Hatchlings of the King Quail (Coturnix chinensis)

Dietary Exposure to Low Levels of Crude Oil Affects Physiological and Morphological Phenotype in Adults and Their Eggs and Hatchlings of the King Quail (Coturnix chinensis)

Metabolic and Hematological Responses to Endotoxin‐Induced Inflammation in Chicks Experiencing Embryonic 2,3,7,8‐Tetrachlorodibenzodioxin Exposure

Energetic basis for bird ontogeny and egg-laying applied to the bobwhite quail

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