Hematopoietic recovery after transplantation is primarily derived from the stochastic contribution of hematopoietic stem cells

Abstract

Reconstitution after hematopoietic stem cell (HSC) transplantation is assumed to occur in two distinct phases: initial recovery mediated by short-term progenitors and long-term repopulation by multipotent HSCs which do not contribute to hematopoietic reconstitution during the first 6-9 months. We have previously reported the transplantation and exclusive engraftment of the HSC-enriched CD34+CD45RA-CD90+ phenotype in a nonhuman primate model. Here, we closely followed the clonal diversity and kinetics in these animals. Enhanced sampling and high density clonal tracking within the first 3 month revealed that multipotent HSCs actively contributed to the early phases of neutrophil recovery and became the dominant source for blood cells as early as 50 days after transplant. Longitudinal changes in clonal diversity supported a stochastic engraftment of HSCs with the majority of HSCs clones vanishing early during neutrophil recovery and a smaller fraction of HSC clones expanding into bigger pools to support long-term hematopoiesis. In contrast to the bi-phasic model, we propose that hematopoietic recovery after myeloablation and transplantation is primarily derived from HSCs in a stochastic manner rather than in two phases by independent cell populations.

The CCR5 Gene Edited CD34+CD90+ Hematopoietic Stem Cell Population Serves as an Optimal Graft Source for HIV Gene Therapy

“…It will be interesting to see whether the small fraction of HPCs in our graft could potentially provide sufficient numbers of early phase neutrophils. On the other hand, a latest report challenges the bi-phasic hematopoietic reconstitution model and provides evidence that HSCs can also contribute to the early neutrophil recovery ( 57 ). Based on this model, CCR5 gene edited CD34 + CD90 + HSCs should result in early reconstitution of hematopoiesis with CCR5 - cells.…”