Hematopoietic Recovery Induced by Bacterial Endotoxin in Irradiated Mice

Smith, Willie W.; Alderman, Ilo M.; Gillespie, Ruth E.

doi:10.1152/ajplegacy.1958.192.3.549

Cited by 79 publications

(12 citation statements)

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“…From these results the authors concluded that the effects of LPS were not dependent on interactions with primary radiation reaction products or that irradiation destroyed the ability of the rodent to respond to LPS. In a follow up paper (Smith et al 1958) the authors reported that recovery of granulocytes, platelets and hemoglobin were improved in mice injected with LPS 24 hours before lethal total body irradiation. LPS given to mice one hour after whole thorax irradiation revealed mitigating effects on lung injury (Zaidi et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Enhanced survival from radiation pneumonitis by combined irradiation to the skin

Gao¹,

Fish²,

Szabó³

et al. 2014

International Journal of Radiation Biology

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Purpose To develop mitigators for combined irradiation to the lung and skin. Methods Rats were treated with X-rays as follows: (1) 12.5 or 13 Gy whole thorax irradiation (WTI) (2) 30 Gy soft X-rays to 10% area of the skin only (3) 12.5 or 13 Gy WTI+30 Gy skin irradiation after 3 hours (4) 12.5 Gy WTI+skin irradiation and treated with captopril (160 mg/m2/day) started after 7 days. Our end points were survival (primary) based on IACUC euthanization criteria and secondary measurements of breathing intervals and skin injury. Lung collagen at 210 days was measured in rats surviving 13 Gy WTI. Results After 12.5 Gy WTI with or without skin irradiation, one rat (12.5 Gy WTI) was euthanized. Survival was less than 10% in rats receiving 13 Gy WTI, but was enhanced when combined with skin irradiation (p<0.0001). Collagen content increased at 210 days after 13 Gy WTI vs 13 Gy WTI+30 Gy skin irradiation (p<0.05). Captopril improved radiation-dermatitis after 12.5 Gy WTI+30 Gy skin irradiation (p=0.008). Conclusions Radiation to the skin given 3 hours after WTI mitigated morbidity during pneumonitis in rats. Captopril enhanced the rate of healing of radiation-dermatitis after combined irradiations to the thorax and skin.

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Section: Discussionmentioning

confidence: 99%

Enhanced survival from radiation pneumonitis by combined irradiation to the skin

Gao¹,

Fish²,

Szabó³

et al. 2014

International Journal of Radiation Biology

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“…The efficiency of haemopoietic recovery after sub-lethal irradiation of endotoxin-treated mice has been well documented (Smith et al, 1958a(Smith et al, , b, 1959Hanks & Ainsworth, 1967b). The present studies were done with the purpose of getting some information about the mode of recovery.…”

Section: Haemopoietic Recovery In Endotoxin-treated Mice After Irradimentioning

confidence: 89%

“…Endotoxin injection either before or after irradiation was found to hasten endogenous recovery of haemopoiesis in mice (Smith, Alderman & Gillespie, 1958a;Smith, Marston & Cornfield, 1959;Hanks & Ainsworth, 1967a). Also, endotoxin treatment of recipient mice after cell transplantation enhanced the spleen colony forming ability of injected CFU (Gidtili, Fehbr & Vhrterbsz, 1969).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Haemopoietic Recovery in Endotoxin‐treated Mice

Sk¹

1976

Cell Proliferation

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Kinetics of mouse spleen colony forming units were studied after intra-peritoneal injection of 1 pglg body weight bacterial endotoxin S. typhosa. When these mice were used as unirradiated and sublethally irradiated donors, it was possible to study the effect of the endotoxin injection upon the cells. Use of the treated mice as irradiated recipients of normal cells gave information about the host effect.In treated unirradiated mice, the total nucleated cell and the CFU counts were disturbed, and 2 days later a large fraction of the CFU were found in the DNA synthesis (S) phase. This meant that injection of endotoxin generated factors affecting the kinetics of the CFU and triggering the resting CFU into the proliferative cycle. If then the mice were given supralethal irradiation and used as recipients of normal bone marrow cells, more CFU seeded to the spleen as compared to normal recipients; but the dip and the growth rate of the CFU were not changed. Hence the endotoxin-generated factors had been eliminated in 2 days.A total body sublethal irradiation by 400 rad X-ray 2 days after endotoxin injection reduced the post-irradiation dip in the recovery curve of the CFU, indicating that though the factors affecting the cell kinetics had been eliminated, the cycling CFU behaved like a growing population. During the first week, the growth rate of the CFU remained the same as in control irradiated mice. The growth rate of the spleen CFU of the endotoxin-treated mice slowed down during the second week, and their self-replicating ability was low. Fluctuations in the DNA synthesizing fraction of the spleen CFU suggested a variability in the ratio of the length of the S phase and the cell generation time.

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“…Windle (27). Recent studies have presented evidence that an increase in mitotic activity of all granulocyte precursor elements occurred after stimulation with pyrogen (28).…”

Section: Methodsmentioning

confidence: 99%